Effects of antibiotics on soil microorganisms: time and nutrients influence pollution-induced community tolerance

A method for the detection of the effects of antibiotics on soil microbial communities was optimised in the present study. We investigated the influence of measurement time and nutrient status on the pollution-induced community tolerance (PICT), using the sulfonamide sulfachloropyridazine (SCP) as model compound. The tolerance development in soil microcosms that were exposed to SCP under different conditions was compared with the background tolerance in SCP-unexposed microcosms. The tolerance of bacterial extracts from the soil microcosms was determined in Biolog^® multiwell plates as the SCP sensitivity of a range of physiological processes. The background tolerance was not affected by soil nutrient amendments, but an influence of the inoculum density in the microtiter plates was observed. Still, the variance of the background tolerance was low, which is in favour of the usage of community tolerance measurements for a selective detection of a toxicant impact. In line with the assumptions of the PICT concept, soil amendment with SCP led to an increase in community tolerance. This tolerance development was enhanced upon additional soil amendment with fresh pig slurry, and less by alfalfa meal addition. Tolerance increases were observed after a soil exposure to SCP of only 7 days, possibly because nutrient input facilitates the fast adaptation of the soil microbial community. However, a further increase in exposure time led to variable changes in the observed tolerance. Prolonged tolerance detection in the microtiter plates (11 days) enabled a clearer differentiation between different soil treatments, as it better resolved the EC₅₀ values of processes with a high tolerance to bacteriostatic antimicrobial compounds. For the detection of antibiotic effects on soil microbial communities, it is therefore recommended to use nutrient amendments (possibly fresh pig slurry), to standardize the soil exposure time, and to extend the period of Biolog plate measurement beyond 7 days.     

ROSAT Detection of an X-ray Shadow in the 1/4-keV Diffuse Background in the Draco Nebula

The detection by the Roentgen satellite (ROSAT) x-ray telescope of a shadow in the 1/4-kiloelectron volt (C band, 0.1 to 0.284 kiloelectron volt) cosmic diffuse background is reported. The location and morphology of the local minimum in x-rays are in clear agreement with a discrete H I cloud. The shadow is very deep with a minimum level at 50 percent of the surrounding emission; therefore, a minimum of 50 percent of the observed off-cloud flux must originate on the far side of the cloud. The analysis of H I velocity components links the cloud with the Draco nebula (distance approximately 600 parsecs); it then follows that there is significant 1/4-kiloelectron volt x-ray emission at a large distance (>400 parsecs) from the galactic plane along this line of sight. The extent of the distant emission region is uncertain, and, if it indicates the existence of a hot galactic corona, it must be patchy in nature.     

A Lunar Occultation of the Dust-Scattering Halo Around GX 5-1 Observed with ROSAT

The x-ray source GX 5-1 in the galactic bulge has been observed with the position-sensitive proportional counter onboard the R?ntgen satellite (ROSAT) during and after a lunar occultation. Extended emission around the source was unambiguously discovered while the central source was behind the lunar rim. This emission is interpreted as a dust-scattering halo around GX 5-1 that has a fractional intensity of 28 percent, implying a grain column density between GX 5-1 and Earth of approximately 3 x 10(10) per square centimeter. The halo derived from imaging during the ROSAT all-sky survey is identical to that obtained from the lunar occultation, thus demonstrating that the ROSAT x-ray mirror scattering has not changed as compared with the mirror properties as measured in preflight calibrations.     

Discovery of microwave emission from four nearby solar-type g stars

Radio waves from the sun were detected 50 years ago, but the microwave detection of other single solar-type stars has remained a challenge. Here, the discovery of four solar-type radio stars is reported. These "solar twin" G stars are radio sources up to 3000 times stronger than the quiet sun. The microwaves most likely originate from a large number of relativistic electrons, possibly produced along with coronal heating, a process that is not understood. Two of the stars are younger than the sun and rotate more rapidly; the dynamo process in the stellar interior is therefore presumably more vigorous, resulting in enhanced coronal activity. One of the detections, however, is an old, metal-deficient G dwarf.