Biocontainment, biosecurity, and security practices in beef feedyards

OBJECTIVE: To determine the biocontainment, biosecurity, and security practices at beef feedyards in the Central Plains of the United States. DESIGN: Survey. SAMPLE POPULATION: Managers of feedyards in Colorado, Kansas, Nebraska, Oklahoma, and Texas that feed beef cattle for finish before slaughter; feedyards had to have an active concentrated animal feeding operation permit with a 1-time capacity of >or= 1,000 cattle. PROCEDURES: A voluntary survey of feedyard personnel was conducted. Identified feedyard personnel were interviewed and responses regarding facility design, security, employees, disease preparedness, feedstuffs, hospital or treatment systems, sanitation, cattle sources, handling of sick cattle, and disposal of carcasses were collected in a database questionnaire. RESULTS: The survey was conducted for 106 feedyards with a 1-time capacity that ranged from 1,300 to 125,000 cattle. Feedyards in general did not have high implementation of biocontainment, biosecurity, or security practices. Smaller feedyards were, in general, less likely to use good practices than were larger feedyards. CONCLUSIONS AND CLINICAL RELEVANCE: Results of the survey provided standard practices for biocontainment, biosecurity, and security in feedyards located in Central Plains states. Information gained from the survey results can be used by consulting veterinarians and feedyard managers as a basis for discussion and to target training efforts.     

Risk factors for initial respiratory disease in United States' feedlots based on producer-collected daily morbidity counts

The incidence of initial respiratory disease was followed for 12 weeks in 122 pens of feedlot cattle, based on producer-collected daily morbidity counts. Weekly incidence density was calculated based on the number of new cases and the population at risk. Incidence density was greatest in the 1st week after arrival and decreased in following weeks. Weekly incidence rate varied between pens and over time from 0 to 27.7 cases per 100 animal weeks at risk. A negative binomial model controlling for multiple events within pens and over time was used to model effects on the number of new cases. Mixed gender groups, cattle from multiple sources and increasing distance shipped were associated with increased risk for initial respiratory morbidity. Heavier entry weight was associated with decreased morbidity risk. These factors may be useful in categorizing groups of calves into risk groups for targeted purchase and management decision making.     

Health risk behaviors and work injury among hispanic adolescents and farmworkers

Adolescents may engage in health risk behaviors that increase their likelihood of injury. Employment places adolescents at risk of work-related injuries. This study responds to the paucity of data on the relationship between adolescent health risk behaviors and work-related injury. This cross-sectional study included the administration of anonymous surveys to ninth graders (n=4914) who attended high schools in south Texas. An aggregate risk score (ARS) was developed based on health risk behaviors. The ARS was analyzed as an outcome using linear regression. Associations between health risk behaviors and work-related injury were assessed with logistic regression. Of the respondents, 19% reported they had a job, and 14% reported that they had been employed in farmwork. Farmwork-related injury was reported by 9% of adolescents, and 12% reported other work-related injury. Mean ARS scores were significantly higher (p < 0.05) for both male and female adolescents who reported a work-related injury compared to nonworking adolescents, and for males who had done migrant farmwork compared to all other adolescent males. The ARS increased as hours worked per week increased. After controlling for confounding factors, a statistically significant association was found between ARS and non-farmwork, work-related injury, but not between ARS and farmwork-related injury. Farmworkers with high ARS were more likely to report non-farmwork, work-related injuries. The predictors of work-related injury in the adolescent groups, particularly for farmworkers and migrants who are under additional stress, remain an important occupational health area to be addressed.     

Larval Bradysia impatiens (Diptera: Sciaridae) potential for vectoring Pythium root rot pathogens

A series of laboratory experiments were conducted to investigate the capacity of Bradysia impatiens (Johannsen) larvae to ingest propagules from two strains each of Pythium aphanidermatum (Edson) Fitzp. and P. ultimum Trow and transmit the pathogens to healthy geranium seedlings on a filter-paper substrate in petri dishes. The capacity of fungus gnat larvae to transmit P. aphanidermatum to seedlings rooted in a commercial peat-based potting mix and germination of Pythium oospores and hyphal swellings before and after passage through the guts of larval fungus gnats were also examined. Assays revealed that Pythium spp. transmission by larval fungus gnats varied greatly with the assay substrate and also with the number and nature of ingested propagules. Transmission was highest (65%) in the petri dish assays testing larvae fed P. aphanidermatum K-13, a strain that produced abundant oospores. Transmission of strain K-13 was much lower (<6%) in plug cells with potting mix. Larvae were less efficient at vectoring P. ultimum strain PSN-1, which produced few oospores, and no transmission was observed with two non-oospore-producing strains: P. aphanidermatum Pa58 and P. ultimum P4. Passage of P. aphanidermatum K-13 through larval guts significantly increased oospore germination. However, decreased germination of hyphal swellings was observed following larval gut passage for strains of P. ultimum. These results expand previous studies suggesting that larval fungus gnats may vector Pythium spp.     

Developing disease-suppressive soils through crop rotation and tillage management practices

A field laboratory was established in Prince Edward Island, Canada, to determine the effects of 2- and 3-year crop rotations, with conventional and minimum tillage treatments, on the severity of soilborne diseases of potato. The 2-year rotation consisted of spring barley and potato (cv. ‘Russet Burbank’), and the 3-year rotation was barley (undersown with red clover), red clover and potato. Examination of potato stem, stolon, and tuber tissues revealed significantly (P=0.05) lower levels of canker and black scurf caused by Rhizoctonia solani, in plants grown in 3-year vs. 2-year rotations. The severity of dry rot (Fusarium spp.) and silver scurf (Helminthosporium solani) was significantly (P=0.05) lower in tubers from plots managed with 3-year rotations and minimum tillage practices. Potato tubers harvested from 3-year rotational soils were significantly (P=0.05) less diseased than those from 2-year rotational soils following inoculation with Phytophthora erythroseptica, causal agent of pink rot. In greenhouse experiments using field soils from 2- and 3-year rotations, we found that potato plants growing in 3-year rotational soils were significantly (P=0.05) less diseased than those growing in 2-year rotational soils following inoculation with P. erythroseptica. Analysis of root zone bacteria recovered from the rhizosphere (exoroot) and potato root tissues (endoroot) showed that the greatest antibiosis activity inhibiting the growth of soilborne pathogens in vitro occurred in bacterial isolates recovered from the endoroot tissues of 3-year rotation crops under minimum tillage management. Our evidence supports the view that soil agroecosystems can be modified through rotation and conservation tillage practices to improve disease suppression by enhancing the antibiosis abilities of endophytic and root zone bacteria (endo- and exoroot).     

Collection and regeneration of populations of wild plants from seed

Four methods of obtaining seeds of wild species of plants for long-term conservation in Gene Banks were compared. (a) From collections growing within botanic gardens; (b) by making sample collections from wild populations and bulking up stocks by regeneration under cultivation; (c) by making arrangements with external collectors; (d) by organising plant collecting expeditions.Collections from plants in botanic gardens were unsatisfactory material on which to base collections. Regeneration under cultivation depends on expertise and knowledge of the plant's cultural requirements which were frequently not available, leading to unacceptably heavy losses of individuals and selection of populations, during cultivation. Collecting seeds directly from populations of wild plants was, scientifically, the most successful method and costings showed it also to be economically the least demanding of resources.The results suggest that, except in special cases—perhaps with rare plants where natural populations are small—every effort should be made to obtain initial collections of seed which are large enough to fulfil all the purposes intended by long-term conservation. Collections of small numbers of seeds which require bulking up before they can be used should be avoided, as a waste of effort, time and plant resources.     

Productivity parameters and soil health dynamics under long-term 2-year potato rotations in Atlantic Canada

Crop rotation effects, due to emerging soil ecological interactions and processes that occur with time, can have a major impact on crop productivity and soil health. 2-year potato (Solanum tuberosum L.) rotation studies were conducted during an 11-year period on a fine sandy loam (Orthic Podzol) under a cool, humid climate in Prince Edward Island in eastern Canada. Rotation partners included Italian ryegrass (Lolium multiflorum Lam.), red clover (Trifolium pratense L.), and barley (Hordeum vulgare L.). The study objective was to assess trends in yield parameters, populations and diversity in nematodes and soil-borne pathogens, and soil structural stability and organic matter retention with time. Italian ryegrass had the largest biomass of both herbage (6.5 Mg ha⁻¹) and roots (4.8 Mg ha⁻¹), compared to red clover or barley. Averaged across 9 years, potato tuber yield was highest under Italian ryegrass (35.0 Mg ha⁻¹) and lowest under red clover (31.1 Mg ha⁻¹) rotations. Differences in tuber yield among rotations was associated with soil N limitation and nematode activity, but was not evident until Year 6. Population densities of specific plant parasitic nematodes recovered from roots and soil were generally low and varied according to host plant. Root lesion (Pratylenchus spp.) and root-knot (Meloidogyne spp.) nematodes were more prevalent under red clover compared to other rotational crops. Stunt (Merlinius spp. and Tylenchorhynchus spp.) nematodes were more common under Italian ryegrass. The main organisms associated with tuber-borne disease were Rhizoctonia solani, Streptomyces spp., Fusarium spp., and Helminthosporium solani. The greatest reduction in tuber-borne diseases occurred with the potato–red clover rotation but significant differences were infrequent. Soil structural form throughout the soil profile (to 30 cm depth) was not adversely affected by the rotational treatments during the duration of the study, while soil structural stability at the soil surface (0–10 cm) was significantly improved. Losses of soil organic C, during the 11-year period ranged from marginal (4%) for rotations with Italian ryegrass, to significant (16%) under the barley rotation. Computer simulations, using the Century model, illustrated the importance of C inputs to maintain soil organic matter levels. Overall, potato crop productivity and soil organic C were generally maintained in rotations that contained Italian ryegrass, but declined under rotations with red clover and barley.