复合微生物制剂对重口裂腹鱼生长、消化酶活性、肠道菌群及水质指标的影响

本试验旨在研究复合微生物制剂对重口裂腹鱼生长、肠道消化酶和菌群以及水质指标的影响.选取健康平均体重(250.00±0.50)g的重口裂腹鱼180尾,随机分成2组(对照组和试验组),每组3个重复,每个重复30尾,对照组饲喂基础日粮,试验组饲喂在基础日粮中添加2‰复合微生物制剂的试验日粮,试验期120 d.结果显示:与对照组相比,饲喂复合微生物制剂可提高重口裂腹鱼的特定生长率和饲料效率,且随养殖时间的增加作用效果越明显;可显著提高前肠、中肠和后肠的蛋白酶、脂肪酶、淀粉酶活性(P<0.01);可显著或极显著增加前、中、后肠芽孢杆菌、双歧杆菌和乳杆菌的菌群数量(P<0.01或P<0.05);可显著或极显著降低试验中、后期水体中活性磷酸盐和氨氮浓度(P<0.01或P<0.05).由结果可知,复合微生物制剂能改善水质,提高重口裂腹鱼肠道消化酶活性、增加肠道有益菌群数(芽孢杆菌、双歧杆菌和乳杆菌),促进鱼体生长.     

Alteration of apoptotic susceptibility and bcl-2 gene in malignant transformation of human bronchial epithelial cells

AIM: To demonstrate the susceptibility of cell apoptosis varies during the progress of cell malignant transformation from human being in vitro. METHODS: A SV₄₀T-transfected human bronchial epithelial immortalized cell line (called M) was selected in this work, which has acquired some characteristics of malignant transformation at the later passage. The alterations of apoptosis and bcl-2, P53 genes between early and later passage of M cells were investigated by means of TDT labeling in situ, chromosome FISH, RNA and protein testing, etc. RESULTS: Incidence of apoptosis induced by cis-platin was significantly lower in later than in early passages of M. Levels of bcl-2 mRNA and protein in later passages were higher than early passages of M, and overexpression of bcl-2 was accumulated following the development of cellular malignancy. P53 protein level was as high in early as in later passages. CONCLUSION: Overexpression of bcl-2 decreases the cellular sensitivity to apoptotic inductors plays an important role during progress of carcinogenesis in human bronchial epithelial cancers. The inactivation of P53 protein in the SV₄₀-T transfected M cell line may be one of reasons of bcl-2 overexpression, but not associated with the accumulation of bcl-2 expressed level during cell transformation.     

Effects of psychological stress on in vitro expression of activated surface molecules on T cells of peripheral blood from healthy persons

AIM: To study cellular and molecular mechanism involved in increasing susceptibility of infection in psychological stress persons. METHODS: Comparative studies were performed with double staining and flow cytometry analysis on immunophenotyping and in vitro expression of early activating surface molecule CD69 in response to mitogens on T cells from peripheral blood of 20 healthy college student volunteers before and after psychological stress. A series of term final examinations was defined as psychological stress. RESULTS: Immunophenotyping analysis showed no statistically significant difference in the percentage of CD2, CD3, CD4, CD8, CD19, CD20, CD16 and CD56 positive lymphocyte populations before and after psychological stress. There was a statistically significant decrease in the in vitro expression of CD69 in response to polyclonal stimulators on the T cells from persons after psychological stress than those before psychological stress. The percentage of CD69 expression (CD69⁺CD3⁺/CD3⁺%) in response to PHA and PDB in the whole blood culture for 72 hours decreased respectively from 28.1±4.1 and 80.7±6.8 on the T cells obtained before psychological stress to 17.6±3.8 and 65.8±7.9 on those obtained after psychological stress, while there was no statistically significant difference between the CD69 expression rates without stimulators on the T cells obtained before and after psychological stress. CONCLUSIONS: The effects of psychological stress to immune system is not on the level of changing proportions of the sub-populations within peripheral blood lymphocytes. Psychological stress can decrease the activating response of T cells in healthy persons, which may be responsible for the increase of susceptibility to infection in the psychological stress persons.     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group （treated with saline; n=10）, EET group （treated with 11,12-EET; n=10） and EET inhibitor 14,15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α （TNF-α）, interleukin （IL）-1β, IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance （HOMA-IR） values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

防治长柔毛野豌豆生草果园杂草茎叶处理剂筛选

【目的】筛选出有效防治果园长柔毛野豌豆生草田杂草的茎叶处理剂,为果园长柔毛野豌豆生草田杂草的化学防治提供参考。【方法】室内盆栽比较了8种除草剂对长柔毛野豌豆的安全性和常见杂草的生物活性,筛选药剂进行复配,进一步研究复配制剂的联合除草类型,测定其对长柔毛野豌豆的安全性及其在长柔毛野豌豆与杂草之间的选择性指数,并对复配制剂进行田间药效试验。【结果】灭草松、咪唑乙烟酸与精喹禾灵茎叶喷雾处理在2×田间推荐剂量下对长柔毛野豌豆安全。咪唑乙烟酸在长柔毛野豌豆与碎米莎草、马唐、牛筋草、狗尾草、播娘蒿、牛繁缕之间的选择性指数大于2.0;灭草松在长柔毛野豌豆与荠菜、播娘蒿、牛繁缕之间的选择性指数小于0.7,安全性差;精喹禾灵在长柔毛野豌豆与牛筋草、狗尾草、马唐之间的选择性指数大于8.8。联合作用测定结果表明咪唑乙烟酸与精喹禾灵按不同质量比复配后对马唐、牛筋草、看麦娘、牛繁缕、播娘蒿、荠菜的联合作用类型均为加成作用,其中以质量比2∶1复配后,选择性指数在2.4～8.9之间。田间试验结果显示：咪唑乙烟酸和精喹禾灵复配后在有效成分125～150 g·hm^-2剂量下,于杂草2～3叶期、长柔...     

关于读者隐性伤害的法律思考

就读者隐性伤害的表现,分析了图书馆相关的法律责任,并提出了防范和解决读者隐性伤害的途径。     

应用纳米磁珠实时荧光定量PCR方法检测番茄黄化曲叶病毒

In order to develop a rapid, sensitive and specific qPCR assay for detection and quantification of Tomato yellow leaf curl virus (TYLCV), a pair of primers and TaqMan probe were designed according to the conserved sequence of known TYLCV isolates. Combining with MNP technique, a novel MNP-qPCR detection method was established and verified based on specificity, sensitivity and reproducibility tests. The results indicated that the Ct value of plotted standard curve showed good linear relationship(R² =0.9994)with the log of copy number of template. The established method showed a high specificity for TYLCV detection without crossing reaction with Tomato severe leaf curl virus and Tomato yellow leaf curl Sadinia virus, and was 10-fold more sensitive than routine PCR. Both coefficients of variation were less than 2%, indicating a good reproducibility. We have provided a novel method for detection of TYLCV in plant samples rapidly and quantitatively.     

设施栽培土壤中盐分积累的测定

通过对通辽市农业科学研究院温室土壤进行室内分析,结果表明：（1）通辽市农业科学研究院温室设施栽培土壤普遍存在酸化现象,其中1号温室土壤酸化已接近蔬菜出现生理障碍的临界水平;（2）所调查温室中土壤均呈现出不同程度的盐化现象,积盐分中阳离子以Ca^2＋为主,而阴离子以SO4^2-和NO3^-为主;1号、4号温室盐化现象严重,其中4号棚中Cl^-含量出现异常,可能与长期施用的某种肥料有关。     

绿洲农田防护林系统土壤蒸发特征研究

以景电灌区绿洲农田防护林系统为研究对象,通过对农田防护林系统内土壤水分蒸发的测定分析,结果表明:在1H范围内,越靠近林带,土壤蒸发量越大。随着与林带距离的增加,土壤蒸发量有先减小后增大的趋势;农田防护林系统内土壤水分蒸发量在灌溉前后有先增加后减小的趋势,并且对照点土壤水分蒸发量始终高于林网内观测点,林网内土壤蒸发量与对照点相比平均减少了35.17%。说明绿洲农田防护林系统能有效减弱土壤蒸发量;相关分析表明,系统内土壤水分蒸发量受到土壤本身和气象因子的制约,其影响程度大小依次为土壤含水量>太阳辐射>空气相对湿度>风速>气温,其中土壤含水量、太阳辐射、气温、风速与土壤蒸发量成正相关,空气相对湿度与其成负相关关系。     

青岛地区保护地小果型西瓜品种比较

以9个小果型西瓜品种为试材,在青岛地区的日光温室内对其生物学特性、丰产性、抗病性、商品性和品质等主要经济性状进行了比较试验。结果表明:"朝霞"、"蜜兰"和"金帅"的产量分别比对照"黄小玉"高35.0%、31.3%和27.3%,风味好,耐贮藏,果实商品率分别为93.5%、94.1%和94.5%。其中"朝霞"果肉颜色特殊,为橘红色,具备特殊的推广价值;"金帅"黄皮红肉,商品性好;"蜜兰"果肉金黄色,综合性状较好。因此,"朝霞"、"金帅"和"蜜兰"可以在青岛地区重点推广种植。