含钼袋控复混缓释肥对土壤酶活性及桃树植株生长的影响

以2年生瑞蟠21/毛桃为试材,通过调控袋控复混缓释肥钼元素含量(0 g/袋:普通袋控复混缓释肥;0.1 g/袋:含钼袋控复混缓释肥),探究含钼袋控复混缓释肥对盆栽桃树土壤酶活性、养分吸收及植株生长的影响。结果表明,与普通袋控复混缓释肥相比,含钼袋控复混缓释肥处理显著提高土壤中蔗糖酶、脲酶、磷酸酶和过氧化氢酶活性,施肥后30,60天分别提高6.99%,7.61%,12.27%,22.86%和6.31%,11.58%,9.05%,32.14%,且差异显著;含钼袋控复混缓释肥处理提高土壤中全钼含量,增加侧根数量,与普通袋控复混缓释肥相比,根系活力、总根长、总表面积和总体积分别提高7.88%,6.12%,3.95%和9.19%;根冠比提高6.92%,干物质总量提高9.99%。与普通袋控复混缓释肥处理相比,含钼袋控复混缓释肥处理植株叶、侧枝和根部全钼、全氮和全磷含量显著提高,但全钾含量无显著差异,并提高叶、侧枝和根部钙、镁、锌和硼等元素含量;显著提高桃树叶片叶绿素含量和净光合速率。研究表明,含钼袋控复混缓释肥可提高土壤酶活性和桃幼树根系活力,增加侧根数量,提高根冠比,并提高植株叶片净光合效率,促进桃树对氮、磷、硼和锌等元素的吸收,从而促进桃树植株的形态建成。     

局部涂抹纳米碳与尿素溶液对桃树梢叶生长及氮素吸收、分配的影响

以7年生的鲁星油桃为试材,采用15 N同位素示踪技术进行田间涂抹试验,研究不同浓度纳米碳与相同浓度尿素溶液(0μg/mL+0.2%,50μg/mL+0.2%,100μg/mL+0.2%,200μg/mL+0.2%,分别以CK、NC50、NC100、NC200表示)涂抹部分叶片对桃树局部梢叶生长、氮素吸收及分配的影响,以期为桃树栽培过程中施用碳纳米提供新的思路和有益的参考。结果表明:与对照相比,施用纳米碳后桃树涂抹叶片单叶面积明显增加,叶绿素含量显著提高,NC200处理下叶片的净光合速率、蒸腾速率和气孔导度比对照提高了15.8%,30.0%,12.4%;纳米碳的施用提高了桃树新梢的干物质积累量,NC100、NC200处理比对照增加了10.5%和12.9%,提高了新梢各部位的全氮含量;高浓度的纳米碳(NC200处理)提高了新生器官对氮素的吸收征调能力(Ndff值);随纳米碳浓度的增大氮素的利用效率显著提高,NC50、NC100、NC200处理的氮素利用率比对照提高了13.6%,29.5%,40.0%;此外,新生嫩叶部分以NC200处理的氮素分配率最高,达19.55%,NC50、NC100、NC200处理氮素分配率比对照提高了4.5%,16.2%,17.1%,差异显著。以上结果表明,纳米碳能够促进新梢叶片对氮素的吸收利用,有效提高叶绿素含量、光合作用效率及新梢局部氮素利用率,影响氮素在梢叶各部位间的分配,促进氮素向生长中心(新生嫩叶)的转移。     

以Lorke法为基础的半致死剂量(LD50)分析方法在油酸、化香树果序、黄连素抗镉减毒评价中的应用

镉污染严重着威胁农业的健康发展,如何有效抗镉减毒是保障农业生产安全的重要措施,然而目前缺乏高效的抗镉药物及简便易行的评价方法。本实验借助Lorke法为基础的半致死剂量(LD50)分析方法,检测了油酸、化香树果序、黄连素等不同潜在的抗镉化合物的减毒作用。通过改良后的Lorke法测定不同抗镉药物对小鼠的径口镉LD50的影响。结果显示,与200mg/kg维生素C相比,在灌胃200mg/kg油酸、化香树果序、黄连素能够减轻镉的毒性,提高镉的LD50。对照的LD50由140-160mg/kg分别上升至300-320mg/kg(油酸组),280-300mg/kg(化香树果序组)和320-340mg/kg(黄连素组)。以上结果表明,以Lorke法为基础的LD50分析方法,能够评价出油酸、化香树果序、黄连素的减镉毒作用,及其剂量-效应关系。以上结果同时证明了相同剂量下黄连素效果要优于油酸、优于化香树果序。     

狼毒根原料特点及其中柱制备糠醛研究

瑞香狼毒是我国北方草原上典型的多年生草本毒植物,资源化利用是新的治理思想和方式。以青海湖南岸的狼毒为研究对象,对其根部原料特征、化学成分和中柱部分制备糠醛进行了测定分析。结果表明:狼毒根皮层厚度与其最大直径呈直线关系;含水量为4.5%时,狼毒根中柱密度(0.72 g/cm~3)略高于皮层密度(0.69 g/cm~3);狼毒根中柱与皮层的化学成分有较大差异。由于狼毒根中柱部分聚戊糖含量较高,适合作为制备糠醛的原料,本研究中糠醛的得率为8.2%,与目前工业常用的原料蔗渣相仿。研究结果可为狼毒资源化提供参考依据。     

云南省昆明市执业兽医考试现状及管理措施

昆明市通过加强考务工作,积极宣传动员,建立长效机制等举措,近年来执业兽医考试工作成效显著,2010—2018年昆明市执业兽医累计报考4 819人,其中(助理)执业兽医师共考取通过1 222人。通过对2010—2018年的考试情况分析发现:昆明市报考人数逐年上升,学历水平不断提升;报考人员职业以动物诊疗机构、学生、非兽医从业人员为主;报考人员通过率以科研教育人员、诊疗机构、学生等居前列。上述情况提示,今后应进一步优化执业兽医注册备案管理,强化行业对执业兽医的重视与认可,完善执业兽医考试和继续教育制度。     

Effect of DNA methylation of miR-30a-5p promoter region on hepatic injury induced by homocysteine

AIM: To explore the role of DNA methylation of microRNA-30a-5p(miR-30a-5p) promoter region in hepatic injury. METHODS: Four-week-old normal mice and cystathionine β-synthase (CBS) single gene knockout mice were used and divided into normal (CBS^+/+, n=12) group and single gene knockout (CBS^+/-, n=12) group, and the mice were fed with high methionine diet for 8 weeks. HL-7702 hepatic cells were routinely cultured in vitro and divided into control group, homocysteine (Hcy) group and Hcy+5-azacytidne (AZC) group. Serum Hcy, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by automatic biochemical analyzer. The levels of ALT and AST in the cells culture medium were determined by the microplate method. Hepatic injury in the mice were observed with HE staining. Cell viability staining was used to measure the viability of hepatocytes. RT-qPCR was used to detect the expression of miR-30a-5p in the liver tissues and hepatocytes. The correlation between the expression of miR-30a-5p and serum ALT and AST levels was analyzed by Pearson correlation analysis. DNA methylation level of miR-30a-5p promoter region in the liver tissues and hepatocytes was detected by nested landing methylation-specific PCR (nMS-PCR). RESULTS: Compared with the CBS^+/+ mice, the serum levels of Hcy, ALT and AST in the CBS^+/- mice were significantly increased (P < 0.05). HE staining showed the hepatocyte swelling and nuclear fragmentation and dissolution. The expression level of miR-30a-5p in the liver tissues was decreased (P < 0.01). Besides, the expression level of miR-30a-5p in the mice was negatively correlated with serum ALT and AST levels (r²=0.4557, P=0.0003, r²=0.4626, P=0.0003), and the DNA methylation of miR-30a-5p promoter region was increased (P < 0.01). In the HL-7702 cells, compared with control group,the ALT and AST levels were increased in Hcy group (P < 0.05, P < 0.01), and the cell viability was remarkablely decreased. DNA methylation of miR-30a-5p promoter region was increased (P < 0.01), which decreased after treated the cells with AZC (P < 0.05), while the expression level of miR-30a-5p in the cells was increased (P < 0.05). CONCLUSION: Hypermethylation of miR-30a-5p promoter region may play an important role in hepatic injury.     

勐腊县珍贵用材树种发展现状与对策研究

总结勐腊县珍贵用材树种发展现状,从地理位置、自然条件、人文条件、政策条件阐述其发展优势。针对勐腊县珍贵用材林发展中存在的土地、技术、投资与收益等问题,提出加大对珍贵树种产业的宣传与示范,加强技术培训与政策扶持,探索以短养长、长短结合的复合经营模式,结合当前林业生态建设项目,推进珍贵树种产业的可持续发展等建议。     

有机地表覆盖材料对树穴土壤理化性质的影响

通过大田试验,研究有机地表覆盖材料对土壤理化性质的影响。以圆明园公园内的桑葚林(Morus alba L.)树穴土壤为研究对象设置6种处理,即水性聚氨酯胶黏剂有机覆盖垫(A)、水性聚氨酯与淀粉胶黏剂混合比9∶1有机覆盖垫(B)、水性聚氨酯与淀粉胶黏剂混合比8∶2有机覆盖垫(C)、水性聚氨酯与淀粉胶黏剂混合比7∶3有机覆盖垫(D)、木片覆盖(M)和无覆盖处理(CK),对不同地表覆盖材料的覆盖效果进行评价。结果表明:(1)总体来看,1年后不同有机地表覆盖材料覆盖下树穴土壤的容重、孔隙度、持水量等物理指标以及不同土层土壤pH变化不大。(2)与CK相比,不同有机地表覆盖材料可增加土壤中有机质、碱解氮、有效磷、速效钾的含量,覆盖后0-10 cm土层比10-20 cm土层的效果更为明显。综合考虑,不同有机地表覆盖材料能够改善土壤养分含量,水性聚氨酯与淀粉胶黏剂混合比8∶2有机覆盖垫(C)为5种有机地表覆盖材料中最适合裸露树穴的覆盖材料。