吉林省玉米杂交种商品品质概述

通过对2004年吉林省市场上推广的134个品种的商品品质进行分析。结果表明,目前吉林省商业杂交种大多数容重都达到了国标二级标准,但晚熟品种整体水平较低。供试品种的子粒平均含水量37.1%。随着熟期的延长,含水量不断增高。     

不同类型土壤中分枝杆菌噬菌体分离率的比较

为了解分枝杆菌噬菌体在自然界的生存环境,深入研究噬菌体在微生态环境中的作用奠定基础。以含柠檬酸和磷酸氢二钠的溶液为提取剂,从50份不同性质土壤中分离、纯化分枝杆菌噬菌体,电镜观察初步确定其分类;统计分析土壤类型、酸碱度、含水量、阳离子交换量、有机碳含量对噬菌体分离率的影响。共分离纯化到13株尾病毒目肌尾病毒科的分枝杆菌噬菌体。3种类型土壤的分枝杆菌噬菌体分离率分别为暗棕壤(41.2%)>黄棕壤(25.0%)>褐土(16.7%);土壤pH值、含水量、阳离子交换量对分离率影响呈规律性:pH值和含水量分别在7.45-7.95和13.7%-21.7%时分离率最高;当阳离子交换量为20.8-28.6 cmol/kg时,分离率随之升高而升高;未见有机碳含量对分离率的影响有明显规律。     

海水鱼消化道菌群结构研究进展

从海水鱼消化道结构、消化道菌群结构、消化道菌群功能及消化道菌群结构研究手段等几个方面综述了海水鱼消化道菌群结构的研究进展。指出海水鱼消化道菌群结构受到生长发育阶段、养殖环境及饵料变化等因素的影响。论述了正常消化道菌群的重要功能,比较分析了目前消化道菌群结构不同研究手段,阐明了消化道微生物分子生态学研究的学术价值。为筛选高效、专一性、可定植性的海水鱼益生菌奠定了基础。     

Application of Principal Component Analysis on Workable Competition Evaluation

The effective competition is an effective organizational form of the industry in modem market economy. It is a form accompanied with large - scale production and vigor competition. The analysis of effective competition has been the focus that people have paid close attention to it very much all the time. It is a basis of industrial policy, on the basis of the former research, the aufhors bring out 9 indexes according to the actual situation of our country, then take automobile trade as an example, we analyze the automobile trade with principal component.     

商品有机肥对水稻生长、产量及稻米品质影响

以上海目前正在推广的商品有机肥为研究对象,以有机无机结合施肥的方式,围绕高产、优质、安全的生产目标,通过田间小区试验,表明在一定量的氮化肥条件下,增施商品有机肥对水稻有显著的增产作用。土壤肥力中等地区,水稻田以基施商品有机肥4500~6000 kg/hm2,再施氮化肥225~255 kg/hm2为宜,从而大幅度地降低氮化肥的用量;通过对稻米品质和土壤分析,明确了增施商品有机肥可提高稻米品质,改善土壤理化性状。     

统筹城乡户籍制度改革与农村人口空心化：挑战与应对

从统筹城乡户籍制度改革对农村资源配置、农村有效投入、农村规模发展及农村技术人才4个方面分析统筹城乡户籍制度改革可能给农村发展造成人口空心化带来的影响和挑战,最后,基于土地流转、农村新型养老保险和发展农业机械化等角度提出了相应的建议。     

三峡库区低碳农业发展模式与减排对策

在对三峡库区自然环境及生态农业发展存在问题分析的基础上,运用类比方法估算了三峡库区农业系统温室气体的排放量,结果显示2008年三峡库区耕地CO2,CH4,N2O的排放量分别为10,7,0.5万t/a。在此基础上进一步提出了山地综合立体开发模式、立体生态渔业养殖模式、水土流失综合防治模式、特色循环生态农业复合模式4种低碳农业发展模式及相应的减排对策。     

Optimization of Stachydrine Hydrochloride in Leonurus Extraction Method by HPLC-ELSD

The study was aimed to extract stachydrine hydrochloride in leonurus by HPLC-ELSD,and establish hydrochloride stachydrine determination methods for providing theoretical basis of leonurus extraction process optimization.The chromatographic conditions:venusil HILIC hydrophilic column;Mobile phase was acetonitrile -0.2% acetic acid solution (volume ratio 80:20);The flow rate was 1.0 mL/min;And the column temperature was 30 ℃;The detector was ELSD and drift tube temperature was 80 ℃ with the atomization chamber temperature 50 ℃ and carrier gas volume flow 1.0000 mL/min.The results showed that there was a good linear relationship when the stachydrine hydrochloride content was 0.49 to 4.91 μg (R²=0.9993);There was the highest rate of active ingredients extraction of when the extraction time was 1 h,material to water ratio was 20:1 and extracted for 2 times.Combined with production practice,the final optimization scheme was that the extraction time was 1.5 h,material to water ratio was 20:1 and extracted for 2 times.In summary,the determination of stachydrine hydrochloride in leonurus should use HPLC-ELSD method which had high specificity,good stability,response and reproducibility and easy to handle.This method could provide the theoretical basis for optimizing the leonurus extraction process.     

超高效液相色谱测定猪配合饲料中苯乙醇胺A的研究

研究建立了超高效液相色谱(ultra performance liquid chromatography,UPLC)—二极管阵列检测器(PDA)测定配合饲料中苯乙醇胺A的方法。通过应用0.1%甲酸甲醇溶液提取、混合阳离子固相萃取净化及样品基质校正外标定量方法进行测量。在优化条件下,苯乙醇胺A在0.1~20.0 mg/kg浓度范围内线性关系良好,线性相关系数(R²)达到0.9945;在1.0、5.0、10.0 mg/kg等不同添加浓度下,方法回收率高于90.36%,变异系数低于5.98%;方法检测限为0.06 mg/kg,定量限为0.10 mg/kg。结果表明,该方法灵敏度高、精密度好、准确可靠且操作简单,能够满足配合饲料中苯乙醇胺A的测定需要。     

饲料脂肪水平对锦鲤幼鱼生长和血液生化指标的影响

本实验以初始体重为(5.85±0.19)g红白锦鲤(Cyprinus carpio L)幼鱼为实验对象,研究在螺旋藻添加量为12%时,饲料脂肪水平对锦鲤生长、饲料表观消化率、肠道消化酶和血液生化指标的影响。实验设5.36%、7.74%、10.52%、12.85%、15.45%5个脂肪梯度,每组设3个重复,每个重复10尾鱼,表观饱食投喂60 d。结果显示:脂肪水平为5.36%～10.52%,锦鲤增重率(BWG)、特定生长率(SGR)随脂肪水平的升高而升高,均在脂肪水平为10.52%时,达到最大值。实验鱼血清谷丙转氨酶(ALT)随脂肪添加比例呈现先下降再升高的趋势,脂肪水平为12.85%后,血清ALT显著升高(P<0.05),脂肪水平为15.45%时,血清GLU浓度显著升高(P<0.05),而甘油三酯(TG)、总胆固醇(CHOL)随脂肪水平的升高各实验组间无显著性差异(P>0.05)。结果表明,螺旋藻添加量一定时,锦鲤适宜脂肪添加水平为10.52%。