鸡ADSL基因和GARS-AIRS-GART基因对鸡肉肌苷酸(IMP)含量的影响

以鸡ADSL基因、GARS-AIRS-GART基因为侯选基因,以隐性白羽鸡、丝羽乌骨鸡、萧山鸡、白耳鸡、藏鸡为试验材料,采用PCR-SSCP方法对ADSL外显子2、GARS-AIRS-GART基因5′侧翼序列进行SNPs检测。结果各发现了一个单核苷酸多态性位点。各种基因型与胸肌IMP含量的最小二乘分析结果表明:ADSL基因TT型个体的胸肌肌苷酸含量极显著地高于CC型、显著地高于CT型个体,CT型个体也高于CC型,但差异不显著;GARS-AIRS-GART基因TT型个体的胸肌肌苷酸含量极显著地高于CT型和CC型个体,CT型个体也高于CC型,但差异不显著。ADSL和GARS-AIRS-GART基因的合并基因型对IMP含量也有显著的影响,合并基因型为TTTT的个体IMP含量显著高于CCCC合并基因型的个体,高出1.584 mg/g,因此可以利用该合并基因型对鸡的肉质风味性状进行标记辅助选择。     

鸡H-FABP基因多态性及其与肌内脂肪含量的相关研究

根据GenBank发表的鸡H-FABP基因序列设计引物,以广东省农科院畜牧研究所提供的4个地方优质肉鸡品种,2个培育品种和1个引进的肉鸡品种为试验材料,通过测序和PCR-SSCP的方法进行SNP检测和基因型分析,探讨H-FABP基因多态性与肌内脂肪含量(IMF)之间的关系。结果发现在H-FABP基因260 bp和675 bp处存在T/C和G/A的突变位点,对2个突变位点在研究群体中进行基因型分析,结果产生3种基因型。基因型与IMF的相关分析结果表明:在T260C位点上,所有品种中AA基因型的IMF含量显著(P<0.05)高于BB基因型个体,但对于不同的品种来说,AA和BB基因型只对封开杏花鸡和岭南黄鸡II号的IMF含量有显著影响(P<0.05),而对其他各鸡种均无显著影响(P>0.05);在G675A位点上,所有品种中AA型和AB型肌内脂肪含量差异显著(P<0.05)。     

农药药械市场2006年概况及2007年展望

1农业形势及农作物重大病虫害发生情况2006年是实施“十一五”规划的开局之年,也是建设社会主义新农村的起步之年。中央出台了一系列惠农政策,农业部提出了实现“三增目标”、实施“三大战略”和“九大行动”的要求,各级植物保护技术推广部门,进一步加大了农作物有害生物防控力度,粮食生产在前两年实现了恢复性增长的基础上,今年继续增产,呈现出良好的局面。2006年全国病虫害偏重发生。小麦条锈病发病早、发病面积大、病情重。小麦蚜虫在黄淮海及北方大部麦区偏重至大发生。水稻螟虫呈重发态势。长江中、下游与江南稻区稻飞虱继续大发生,为…     

Influence of murine cytomegalovirus infection on the differentiation and the differentiation genes expression in neural stem cells

AIM: The influence of MCMV infection on differentiation and differentiation gene expression in neural stem cells(NSCs) in vitro were investigated for studying the mechanisms of brain abnormalities caused by congenital cytomegalovirus infection.METHODS: NSCs were separated from fetal BALB/C mouse, and cultured and identified in vitro. The differentiation potency of NSCs was observed by immunofluorescence. The NSCs infected by MCMV at dosage of MOI(multiplicity of infection) equaled to 5, 1 and 0.1,respectively, were cultured in differentiation medium. The morphological changes of infected cells were observed under inverted microscope. The ratios of NSCs and its differentiated cells were detected by flow cytometry. The expressions of nestin, GFAP and NSE, markers of NSCs and its differentiated cells, were studied by immunofluorescence(MOI=1). The expression of early antigen(EA) of MCMV was detected to observe the infection process. Real-time RT-PCR method was employed to measure the expression levels of the key genes Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs at early stage of differentiation culture.RESULTS: NSCs isolated from embryonic mouse brains proliferated to form neurospheres, strongly expressed nestin and differentiated into NF-200 positive neurons or GFAP positive astrocytes. The infected NSCs did not adhere to the wall and appeared differentiation growths, but showed swollen gradually after differentiation culture. The nestin expression in the infected cells downregulated slowly and was higher than that in control groups(P<0.05). The GFAP and NSE expressions of the infected cells were lower than those in control groups(P<0.05). The early antigen(EA) of MCMV was always detected in the cells in infected groups. The ratios of nestin positive cells in infected groups were higher than those in control groups, but the ratios of GFAP and NSE positive cells of former were lower than that of the latter from 3rd to 9th d after differentiation culture(P<0.05). The levels of Neurog2 mRNA and Myc mRNA in infected groups were markedly lower than those in normal control groups on 1st d and from 1st to 4th d after differentiation culture, respectively(P<0.05). The levels of Ccnd1 mRNA of infected groups were obviously lower than those in normal control groups from 12th h to 1st d(P<0.05). These changes in infected groups became more obvious as MCMV MOI increased.CONCLUSION: MCMV significantly inhibits differentiation of NSCs to neurons and astrocytes, and leads to the decrease in differentiated cells. MCMV inhibits or interferes with the gene expression of Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs. The effect that MCMV inhibits the expressions of differentiation and the differentiation genes in NSCs shows dose-dependent with MCMV MOI. The inhibitory effect of MCMV on the differentiation of NSCs might be induced by interfering with the expression of differentiation gene in NSCs, which is possibly the one of primary causes of brain development disorders induced by congenital CMV infection.     

木香花挥发性组分的定量结构-色谱保留关系研究

采用分子电性距离矢量(MEDV)表征了木香花挥发性成分的分子结构,建立了色谱定量结构-保留关系(QSRR)模型,同时运用多元线性回归/逐步回归对模型进行变量筛选,建立了53种木香花挥发性成分气相色谱保留时间(tR)与MEDV的定量相关10 变量、 6变量模型及线性保留指数(ILR)的10、7、6变量模型.QSRR模型的建模计算值复相关系数(R)分别为0.949、0.906、0.906、0.949和0.943.留一法交互校验复相关系数(RCV)分别为0.867、0.904、0.905、0.901和0.904.结果表明所建模型具有良好估计能力与稳定性.     

池塘青虾高产养殖技术

青虾，学名日本沼虾，肉质细嫩，味道鲜美，是近年来出现的新的养殖品种。因其养殖设施简单、投资少、效益高、市场容量大等特点，近年来池塘青虾养殖面积在杭嘉湖地区得到大规模的发展。目前全省养殖面积已达８万多亩，养殖技术日趋完善，养殖产量和养成规格不断提高，所取得的经济效益也十分明显。一般亩产青虾７５—１００ｋｇ，亩利润１５００—２５００元，高的亩产量可达１５０ｋｇ以上，亩利润高达４０００多元。现将在池塘青虾养殖过程中所采取的主要技术措施总结如下。１虾塘条件１．１面积 １—４亩较适宜，太小易缺氧，太大管理…     

郑州牧专女教职工妇科病普查结果分析

近几年来,我校十分重视教职工的身体状况,每年要对女教职工妇科疾病普查1次,每两年对全体教职工身体状况全面检查1次。我校于2006年12月对已婚女教职工进行了妇科病普查,现将基本情况如下。     

2022年夏季汉江中下游水华生消驱动因子及其贡献率量化研究

2022年夏季，长江流域遭遇了罕见的“汛期反枯”极端水文事件，在此期间，汉江中下游首次发现了蓝藻水华。本研究选取叶绿素a浓度作为衡量水华的关键指标，基于偏最小二乘回归（Partial least squares regression，PLSR）量化了不同环境因子对2022年夏季汉江中下游（仙桃、宗关断面）水华生消的贡献率。结果表明：（1）仙桃和宗关断面叶绿素a浓度与溶解氧、pH值和水温均呈现出显著的正相关关系。溶解氧、pH值和水温对2022年夏季汉江中下游水华生消的贡献程度最高，三者对仙桃和宗关断面水华生消的贡献率分别为15.18%、13.68%、14.50%和18.06%、15.93%、15.65%。（2）基于偏最小二乘路径模型（Partial Least Squares Path Modeling，PLS-PM），本研究进一步解析了各环境因子对叶绿素a浓度变化的影响路径。结果表明气象因子是2022年夏季汉江中下游蓝藻水华暴发的诱导因子，高温无雨的极端天气导致水温和pH上升，加速了藻类的代谢反应速率。同时，河道流量减小延长了有机物和营养盐的传输和滞留时间，为藻类生长提供了稳定的营养条件。并且水华“萌发”时段可能与汉江中下游“涝旱急转”时段重叠。因此，本文建议下一步研究应结合准确的中长期气象预报信息，在“涝旱急转”时段实时优化汉江中下游水华防控调度的下泄流量与下泄时机，这将有可能大大提高水华防控的效率和效果。     

晋单51号玉米夏播高产栽培技术

晋单51号是运城市盐湖区种子公司选育的紧凑型、高产、活秆成熟的中早熟玉米单交种。该品种2006年通过山西省审定,审定编号:晋审玉2006027。1特征特性1.1农艺性状晋单51号春播生育期118天,夏播95天,需要≥10℃活动积温2300～2400℃。叶鞘紫色,叶色深绿,成株株型紧凑,株高245㎝左右,穗位高105㎝左右,果穗长约17㎝,果穗粗约5.2㎝,穗行数16或18行,行粒数40粒左右。子粒黄色,半马齿型,百粒重38g,出子率88%。最大特点是植株保绿,活秆成熟,耐密植,果穗结实性好,秃尖小,品质好,群体果穗里外均匀一致。