普通野生稻稻米加工品质和外观品质性状QTL定位

本研究利用一套以籼稻品种“特青”为遗传背景的云南元江普通野生稻（Oryza rufipogon Griff.）渗入系为材料，采用单标记回归分析和渗入片段叠代法，对出糙率、整精米率、垩白粒率、垩白度、长宽比等5个品质性状的QTL进行了分析，初步定位了16个QTL，有10个QTL来自野生稻的等位基因能改良群体的品质性状。在第5染色体RM289附近检测到了同时影响长宽比、垩白粒率QTL，来自野生稻的等位基因能增加长宽比、降低垩白粒率，贡献率也较高。在第8染色体RM152附近检测到降低垩白粒率和垩白度的QTL，其贡献率分别为14％和9%。本研究结果不仅为品质性状分子标记辅助选择提供参考，而且充分显示了利用野生稻的优异基因改良栽培稻品质性状的巨大潜力。     

混合像元分解研究综述

混合像元是遥感领域研究的热点,混合像元的问题若得不到很好的解决,将会给遥感的后续应用研究带来较大的误差.本文简要介绍目前国内混合像元的主要分解方法:有线性光谱混合模型、非线性光谱混合模型、模糊监督分类法和神经网络模型分类法,并对其优缺点进行了简要说明.     

Prokaryotic Expression of Gly-Gln Dipeptide and Its Bioactive Analysis: A Novel Method for Short Peptide Production

Small bioactive peptides, with diverse biological functions, have received increasing attention as physiologically beneficial substances in animal production. The main obstacle to wide application of small bioactive peptides is a lack of cost-effective methods for mass production. In this study, we used glycyl-glutamine (Gly-Gln) as a test case to develop a novel mass-production method for small bioactive peptides. The oligonucleotide encoding Gly-Gln pro-peptide (Gpp) was designed and synthesized. Gpp includes 3 Gly-Gln dipeptides and 2 enzymatic sites for pepsin and trypase, allowing direct digestion and absorption of Gly-Gln in the gastrointestinal tract. The Gpp oligonucleotides were linked to generate an oligomeric oligonucleotide segment containing 12 tandem copies of Gpp. This 12Gpp segment was cloned and expressed in Escherichia coli vector pET32a. By optimizing culture conditions [0.1 mmol L-1 isopropyl-β-D-thiogalactopyranoside (IPTG), 50 lag mL-1 ampicillin (Amp), 30℃ for 12 h], the thioredoxin fusion peptides reached 40% of total bacterial protein. After purification, the fusion protein was fed to Kunming mice to determine its effect on mouse immune function. The results showed that similar to Gly-Gln dipeptide, Gpp polymer protein could significantly suppress the proliferation of T and B lymphocytes in blood and spleen, and additionally could significantly improve interleukin-2 (IL-2) and interleukin-6 (IL-6) secretion of blood and spleen lymphocytes. These effects were not observed in mice fed a 2 amino acids mix (glycine and glutamine). These evidences indicated that an efficient digestion of Gpp polymer protein could be achieved when ingested into the animal gut. The expression system in this study provides a potential production method for not only Gly-Gln dipeptide but also other short bioactive peptides.     

生长抑素基因疫苗对肉用鸡生长性能及相关激素的影响

将28日龄的150只肉鸡随机分为5个组,每组30只,A、B、C组分别口服以减毒沙门菌为载体的生长抑素基因疫苗pcS/2SS,剂量分别为8×109、4×109、1×109 CFU/只,D组口服生理盐水,作为对照组,E组口服减毒沙门菌CS022,2周后加强免疫1次。研究生长抑素基因疫苗对肉用鸡生长相关激素和抗生长抑素抗体的影响。结果显示,以减毒沙门菌为载体的生长抑素基因疫苗pcS/2SS口服疫苗免疫肉鸡后,各试验组日增重有升高趋势,C组日增重分别比D、E组提高了10.7%和9.5%(P<0.05),而且C组肉鸡饲料利用率较高,分别比D、E组提高了10.9%和15.4%。与对照组(D、E组)相比,C组血浆GH浓度明显升高(P<0.05),试验组血浆T3含量有上升趋势;而A、B组GH、T4、T3与对照组差异不显著(P>0.05)。各试验组抗SS抗体在加强免疫后2周时达到最高,各试验组P/N值均达到阳性,加强免疫后4周时,抗体水平有所下降,其中C组肉鸡抗体P/N值为阳性,A、B组肉鸡此时均值未达到阳性。结果表明,口服生长抑素基因疫苗可以提高肉鸡的日增重和饲料转化率,增加肉鸡血浆GH、T3浓度,提高生长抑素抗体P/N值,...     

淮安市经济开发区工业用地管理实践与思考

开发区工业用地管理对工业用地集约和高效利用以及促进地方经济持续、快速发展有重要作用。以淮安市经济开发区为例,总结了开发区成立以来工业用地管理的效益、经验与不足,并结合发达地区开发区的经验教训,从土地节约、集约利用,土地管理组织机构和工业用地管理机制以及园区规划等方面,提出了加强开发区工业用地管理的对策建议。     

8种杀菌剂对9种薄壳山核桃炭疽病病原菌的毒力测定

目的 测定8种杀菌剂对9种炭疽菌（26个菌株）的室内毒力,筛选出防治薄壳山核桃炭疽病的高效药剂。 方法 采用菌丝生长速率法测定8种杀菌剂对26株炭疽菌菌丝生长的抑制作用,构建毒力回归方程,并计算EC50值。 结果 供试8种杀菌剂中,咪鲜胺和咯菌腈对所分离的9种炭疽菌（26个菌株）菌丝生长的抑制作用最强,平均EC50值分别为0.14、0.15 mg·L−1。戊唑醇对C. fioriniae、C. liaoningense、C. tamarilloi和C. americae-borealis菌丝生长的抑制作用较强（平均EC50 = 0.49 mg·L−1）,但对另5种炭疽菌效果不明显（平均EC50 = 23.60 mg·L−1）。恶醚唑对C. nymphaeae、C. siamense、C. alienum、C. fioriniae和 C. coelogynes菌丝生长的抑制作用较强（平均EC50 = 0.81 mg·L−1）,但对另4种炭疽菌抑制效果一般（平均EC50 = 2.00 mg·L−1）。代森锰锌对9种病原菌菌丝生长的抑制性最差,平均EC50值为42.70 mg·L−1。 结论 咪鲜胺、咯菌腈对薄壳山核桃炭疽菌具有较高的毒力,在薄壳山核桃炭疽病林间防治中具有较大的应用潜力。为避免产生抗药性,建议轮换使用咪鲜胺、咯菌腈、恶醚唑和甲基硫菌灵等杀菌剂。     

玉米主产区利益流失与补偿机制研究

在简述我国玉米主产区利益流失根源的基础上,系统分析以吉林省为代表的玉米主产区在商品粮生产经营过程中存在的利益流失问题,探讨显性利益流失和隐性利益流失的形态,并以吉林省为例测算显性利益流失的数量,在此基础上构建玉米主产区利益补偿机制,明确玉米主产区利益补偿的主体和受体、补偿标准以及现金补偿与政策性补偿的运行机制及实施问题。     

苎麻地膜覆盖高产栽培技术试验示范及推广

本文就达州市苎麻地膜覆盖栽培技术的试验示范和2003年、2004年推广应用该技术的情况做了介绍；目前该技术已成为该市发展苎麻生产的一项重要技术措施。     

Mutagenic Improvement of Shattering Characteristic of the Restorer Line of a Hybrid Rice “Xieyou 9308“

The newly developed hybrid rice combination “Xieyou 9308“ (Xieqingzao A/T9308) has been regarded as a super-high-yielding rice variety, of which the yield potential reached as high as 12 t/ha. However, its high shattering characteristic (as high as 60%) has limited its wider application. In the current experiment, a non-shattering mutant line, M9308, was developed from T9308 by gamma irradiation. Comparisons were made but no marked differences were found between T9308 and M9308 as well as between their F1 hybrids crossed to Xieqingzao A for major agronomic and grain quality characters as well as resistance to diseases. Genetic analysis indicated that the non-shattering character of M9308 was governed by a single recessive gene.     

5-氮胞苷介导的转基因crylAb的阶段复活及其对水稻的生物学效应

用不同浓度的5-氮胞苷溶液配合不同时间处理了发生在转录水平的转基因cry1Ab沉默水稻。结果表明，5-氮胞苷处理能使8%～30%转基因水稻植株的转基因cry1Ab恢复表达活性，复活转基因表达的Cry1Ab蛋白含量最高可达0.147%，45mg/L5-氮胞苷处理1d可使转基因cry1Ab的复活率及Cry1Ab蛋白含量达最高。同时，5-氮胞苷处理使转基因水稻植株出现矮化，分蘖数、单株粒重下降，穗长减小及开花期延迟等发育异常现象。5-氮胞苷作为一种弱诱变剂在克服转基因沉默及创造具优良农艺性状的转基因植物中具有一定的应用价值。