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61.
不同施肥模式对玉米各器官碳氮累积和分配的影响 总被引:3,自引:0,他引:3
以长期定位试验为依托,选取:1)不施肥(CK);2)农民习惯施肥(FP);3)推荐施肥(OP);4)有机肥氮替代100%化肥氮(OM);5)有机肥氮替代50%化肥氮(MF)5个处理,研究不同施肥模式对玉米植株及各器官碳氮含量、碳氮分配比例及C/N的影响,为西南紫色土地区合理施肥、作物增产提供科学依据。结果表明,与FP处理相比,MF处理显著增加了玉米植株生物量,达26.2%。相比OM和OP处理,MF处理显著增加玉米苞叶和根茬中碳浓度,分别增加5.4、4.2 g·kg-1和7.4、21.3 g·kg-1,同时增加玉米苞叶、根茬、穗轴和籽粒中的碳储量,玉米茎秆和籽粒中的氮储量也有增加。此外,相比FP处理,MF处理能显著增加玉米整株的碳储量和氮储量,达29.1%和16.9%。等氮水平下,MF、OP处理均能增加玉米苞叶和籽粒中碳同化物的分配比例,MF处理玉米籽粒中氮素的分配比例较OP和OM处理分别增加1.7%和3.6%,同时MF处理能使玉米维持较高的C/N。综上,有机肥氮替代50%化肥氮能增加玉米植株的生物量,同时提高玉米对氮素的吸收和碳素的累积,增加玉米籽粒中碳同化物和氮素的分配比例,同时,有机肥氮替代50%化肥氮能使玉米植株维持较高的C/N,有利于产量的形成,该施肥方式不仅能够促进氮素的高效利用,减少化肥的投入,还能够减少化肥损失,降低氮素损失引发的环境风险。 相似文献
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为搞清湿地土壤驱动N2O排放的关键氮源类型,有效减少湿地N2O的排放,本文通过室内控制温湿度,用气相色谱法分析不同外源氮素对湿地N2O排放的影响。结果表明:外加氮源组总是高于对照组N2O排放量(4.4 mg·m-3)。在设定的剂量范围内,单独添加尿素或尿素与硝酸铵1∶1配合时N2O排放量呈现先增后减的单峰分布趋势,峰值分别为10.6 mg·m-3和229.0 mg·m-3;单独添加硝酸铵时N2O排放量(32.6~111.0 mg·m-3)随着氮素添加量增加呈现持续上升趋势。单独添加尿素或硝酸铵、尿素与硝酸铵1∶1配合均促进N2O的排放,但硝酸铵尿素混合添加对N2O排放量的贡献单独添加硝酸铵单独添加尿素。这为预测内蒙古高原区农牧交错带湿地氮素输入可能带来的温室效应和有效减排提供科学依据。 相似文献
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LEI Xiao-can CUI Kui-qing SU Jie LI Zhi-peng ZHAO Xian-hong LIU Qing-you SHI De-shun 《中国畜牧兽医》2015,42(9):2215-2223
Buffalo BMP1 gene was cloned in the present study, the BMP1 sequence was systemically analysed by bioinformatics techniques, and the expression level of BMP1 gene in different tissues were also assayed with Real-time fluorescence quantitative PCR (QRT-PCR).The results showed that with RT-PCR a 3 195 bp buffalo BMP1 gene was cloned and sequenced, including the whole ORF of 2 967 bp (coding 988 amino acid).The sequence multialigned results showed that buffalo BMP1 gene shared 99%, 96%, 96%, 96% and 95% of similar amino acid sequence with Bos taurus, Sus scrofa, Equus, Homa sapiens and Mus musclus, respectively.It was predicted that buffalo BMP1 protein contained a signal peptide domain, a preregion, a metalloproteinases domain, five complement-Uegf-BMP-1 domain (CUB domain) and two epitheloid growth factor-like domain (EGF-like domain).In addition, we also analyzed the expression level in different tissues through QRT-PCR, the results showed that BMP1 gene mRNA existed in all nine tissues with the most abundant expression in heart, followed by testis, ovary, genital ridge, while with lower amount of bone and other tissues, the minimal expression in liver was observed. 相似文献
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MAO Ran LI Qing-quan SHI Yan LAN Xiang-li WANG Min ZONG Ze-jun XIA Guo-zhong HAN Tian-long 《中国畜牧兽医》2015,42(12):3308-3314
In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state. 相似文献
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以微拟球藻为原料,研究微藻水热液化过程中多种参数对水热液化后有机物回收率的影响,包括温度、时间、溶剂比和pH等,从而获得微藻水热液化后有机物的最佳回收工艺条件。结果显示,在室温下进行有机相回收,保证回收溶剂比为20∶1(溶剂/水相不溶物;v/wt)、停留时间为10min、回收pH7,即可达到最佳回收效果。最佳条件下水热液化有机相的回收率为34%左右,且不同的回收温度和pH值对回收的有机相组分均会产生影响。 相似文献
70.
Chun-Zhi REN Wen-Yue HU Jun-Cheng LI Ying-Hong XIE Ni-Na JIA Jun SHI Ying-Yi WEI Ting-Jun HU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1781
Pseudorabies virus (PRV) infection leads to severe inflammatory responses and tissue damage, and many natural herbs exhibit protective effects against viral infection by modulating the inflammatory response. An ethyl acetate fraction of flavonoids from Polygonum hydropiper L. (FEA) was prepared through ethanol extraction and ethyl acetate fractional extraction. An inflammatory model was established in RAW264.7 cells with PRV infection to evaluate the anti-inflammatory activity of FEA by measuring cell viability, nitric oxide (NO) production, reactive oxygen species (ROS) release, and mRNA expression of inflammatory factors, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Its functional mechanism was investigated by analyzing the phosphorylation and nuclear translocation of key proteins in the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Our findings indicate that PRV induced inflammatory responses in RAW264.7 cells, and the responses were similar to that in lipopolysaccharide (LPS)-stimulated cells. FEA significantly suppressed NO synthesis and down-regulated both expression and secretion of COX-2, iNOS, and inflammatory cytokines (P<0.05 or P<0.01). FEA also reduced NF-κB p65 translocation into the nucleus and decreased MAPK phosphorylation, indicating that the NF-κB/MAPK signaling pathway may be closely related to the inflammatory response during viral infection. The findings suggested the potential pharmaceutical application of FEA as a natural product that can treat viral infections due to its ability to mitigate inflammatory responses. 相似文献