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871.
ZHAO Ya-jun XU Chang-qing SHI Sa SUN Hong-li WANG Ning LI Bao-xin WANG Ling YANG Bao-feng Rui Wang 《园艺学报》2005,21(10):1938-1941
AIM: To study the effect of extrogenous low concentration polyamine on cardiomyocyte calcium overload caused by anoxia and reoxygenation. METHODS: Enzymatically isolated rat ventricular myocytes were perfused with normal Tyrode solution for 8 min, then change to anoxia solution for 32 min, at last back to normal Tyrode solution perfusion for 8 min to establish the cardiomyocyte model of anoxia and reoxygenation. Spermine was added extracellularly to the bath before anoxia and spermine, spermidine or putrescine was added extracellularly after reoxygenation. Intracellular calcium fluorescence intensity (FI) was measured continuously by laser scan confocal microscope (LSCM). RESULTS: In the unstimulated state, exogenous spermine (1 mol/L) did not change resting [Ca2+]i in the rat cardiomyocytes. Adding spermine before anoxia antagonized the [Ca2+]i elevating caused by anoxia/reoxygenation. Adding spermine after reoxygenation also lowed the enhanced [Ca2+]i caused by reoxygenation. Considering the potency of two conditions, the former was more efficacious than the later. Spermidine and putrescine also lowed the enhanced [Ca2+]i caused by reoxygenation, but they were less efficacious than spermine. CONCLUSIONS: The results indicate that spermine given before anoxia or after reoxygenation, antagonized or lowed the cardiomyocytes calcium overload caused by anoxia/reoxygenation, but the later was weaker than the former. The order of potency of the polyamine lighten cardiomyocytes calcium overload caused by anoxia/reoxygenation was spermine>spermindine>putrescine. 相似文献
872.
WANG Zhi-jian DING Wen-hui SHI Li-bin MENG Lei REN Zi-wen PU Ding-fang ZHANG Yong-gang TANG Chao-shu 《园艺学报》2005,21(11):2081-2085
AIM: To investigate the expression of the urotensin Ⅱ (UⅡ) receptor GPR14 in the aorta of apoE knockout mouse. METHODS: The expression of GPR14 in the aorta of apoE knockout C57BL/6J mice at various ages (18 weeks, 28 weeks, and 38 weeks old, respectively) was determined with competitive RT-PCR. A binding assay of [125I]-UⅡ on the aortic tissue was also performed in 28 weeks group. RESULTS: We found significant upregulation of GPR14 mRNA at all three ages. Compared with wild type group at the same age, the GPR14 mRNA level in apoE knockout mice increased 54.2% in 18 week group (P<0.05), 50.0% in 28 weeks group (P<0.05) and 97.0% in 38 weeks group (P<0.01). In the knockout group or in the wild type group, expressions of GPR14 in the 28 weeks time point were significantly higher than that in other two age groups, and there was no difference between the 18 weeks and 38 weeks group. In the binding assay, the Bmax of [125I]-UⅡ to the aorta of apoE knockout mouse at 28 weeks increased 64% compared with the wild type (P<0.01), and no difference about the Kd between the two groups was observed. CONCLUSION: UⅡ and its receptor probably play an important role in the development of atherosclerosis. 相似文献
873.
SHI Dun-yun ZHANG Qiong-li LI Yu-zhu LI Ming XU Yun ZHUO Jia-cai DU Xin WANG Ming-chun 《园艺学报》2005,21(11):2264-2267
AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38- and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers. 相似文献
874.
875.
AIM: To study the effects of intrathymic inoculation of liver specific antigen (LSA) on hepatocyte apoptosis after liver allotransplantation. METHODS: Orthotopic liver transplantation was used in this study. Group Ⅰ: syngenic control (Wistar-to-Wistar); Group Ⅱ: acute rejection (SD-to-Wistar); Group Ⅲ: thymus inoculation of SD rat LSA day 7 before transplantation. The observation of general situation and survival time, hepatocyte apoptosis and LAT expression in liver transplants were used to analyze immune state of animals in different groups. RESULTS: The general situation of group Ⅰ was very well after transplantation. Recipients of groupⅡ lost body weight progressively and all died within day 9 to day 13 post transplantation. As for group Ⅲ, the general situation of recipients was remarkably better than that in group Ⅱ. The positive cells of apoptosis in group Ⅲ detected by TUNEL were not significantly different from that in group Ⅰ, but was significantly lower than that in group Ⅱ. LAT was detected at any time in group Ⅱ with peak expression at day 5 and day 7 post transplantation. In contrast, LAT was not detected in any other groups. CONCLUSION: Intrathymic inoculation of LSA protects hepatocytes from apoptosis after liver allotransplantation. 相似文献
876.
西北地区生态农业建设的思考 总被引:6,自引:1,他引:6
针对西北地区生态农业背景、特点,分析了生态农业建设在西部开发中的地位,认为西北地区生态农业建设的基本思路应以水为度,量水而行,节水为本;立足生态环境保护,发展效益型的生态种养模式;推广特色种植,绿色加工,以本土化实现国际化;加强居民建设,实现跨越式发展目标。其中的关键举措有建立节水工程,发展节水高效农业;以经济林(草)建设为中心,提倡人工植物种草。控制土地“三化”;兴办区域化精品产业,搞好精细加工;按照趋利避害原则,取长补短,发展设施农业。 相似文献
877.
旱作春小麦品种高产抗旱特性的综合评价 总被引:11,自引:0,他引:11
对引进的旱作春小麦品种(系)做小区试验,初步筛选出C160和8275两个抗旱性强产量高的品种(系)。其叶面积变化表现为峰值出现早、大小适中,后期能保持较高叶面积。通过生理指标测定,在干旱条件下,这两个品种(系)均表现为叶绿素高、叶片相对含水量多、脯胺酸积累增加、叶水势加大、细胞膜的相对透性减少等特征,是较抗旱的表现,运用模糊综合评判法将各种的产量、与产量相关的性状和生理指标结合在一起进行综合评价,对品种优劣进行排序,其顺序为:C160,8275,8139,8624,8338,定西35,C4,昌春3号,会宁17。 相似文献
878.
879.
880.
农户家庭生命周期及其经济运行研究 总被引:7,自引:1,他引:6
家庭组织是农户特有的一处经济组织形式,本研究运用全国农村固定观察点山西太谷县武家庄村的调查资料,着重研究了农户经济状况和家庭决策模式与农户家庭生命周期的关系。结果表明:两者都与农户家庭生命周期密切相关。为此,应当对农户家庭生命周期规律引起高度的重视。 相似文献