酰胺类除草剂及其安全剂研究进展

本文综述了酰胺类除草剂的概况、作用机理及其安全剂的研究进展,并展望了酰胺类除草剂及其安全剂的发展前景。     

Decreased fibroblast proliferation and collagen content in the wound of diabetic rats caused by streptozotocin

AIM: To study fibroblast proliferation and collagen synthesis during wound healing in diabetic rats induced by streptozotocin. METHODS: 30 Wistar male rats were randomly divided into control group and model group. 55 mg/kg STZ were given intraperitoneally to model rats. After 3 weeks, a round skin of 2.04 cm2 was excised on all dorsal back of rats. The healing time and healing rate were observed according to re-epithelization. The numbers of fibroblasts and the expression of proliferating cell nuclear antigen (PCNA) were observed by Hematoxylin-Eosin (HE) staining and immuno-histochemistry assay. Collagen Ⅰ and Ⅲ stained by Picric acid-Sirius red were calculated by image analysis. RESULTS: The healing time in model group was (27.13±1.81) days，significantly longer than that in control group ［(15.25±1.67） days， P<0.01］. The healing rates in model group were significantly less than that in control group at day 3, day 7 and day 15 (P<0.01). The amount of fibroblasts and the expression of PCNA in model group were significantly less than those in control group on day 3, day 5, day 7 and day 9, respectively (P<0.05, P<0.01). Even the content of collagen I in the wound of both groups increased with time, the values were much higher than that in model group at different times (P<0.05), respectively. For model group, the ratio of collagen Ⅰ/Ⅲ was less than that in control group 3, 7 and 11 days after wound (P<0.01). CONCLUSION: STZ impaires wound healing in rats, which is possible caused by the disturbance of fibroblast proliferation and collagen synthesis in the wound.     

南昌地区瓢虫柄腹姬小蜂生物学特性的研究

瓢虫柄腹姬小蜂在南昌室内,5月下旬至12月上旬可繁殖11代,每代需12～46天。该蜂的发育起点温度为5.28℃。有效积温296.85日度。在28℃下,饲以10％蜂蜜,成蜂平均寿命为5.52天,不喂蜂蜜平均约缩短3天。该蜂能寄生28星瓢虫幼虫的各个龄期,但更喜寄生高龄幼虫,以4龄幼虫作寄主,每雌一生能寄生幼虫6.21头,子蜂数平均为74.57头。可行孤雌生殖产雄。在马铃薯上的自然寄生率以6月份最高,平均为28.47％。在龙葵上以7月份最高平均达64.5％。7月中旬由于高温的影响,寄主和寄生蜂的发育受到抑制,寄生率急剧下降。     

DETERMINATION OF THE VARIETAL TOLERANCE OF SUGARCANE TO PRE-EMERGENCE DIURON AND ATRAZINE

Summary. Six sugarcane varieties, N: Co 310, F146, F148, F152, F153 and F156, were Found in a field trial to differ in their tolerance to pre-emergence diuron with units of susceptibility of 100, 1·57, 1·20, 1·60, 11·30 and 8·07, and to pre-emergence atrazine of 0·75, 0·97, 0·59, 1·44, 2·42 and 0·78 respectively. Relative tolerance was assessed by subjecting each variety to seven rates of the herbicides in geometric series and calculating the F values for monthly measurements of the cane plants through the growing seasons of both the autumn-planting and ratoon crops. Units of susceptibility of each variety were obtained by transforming each mean F value into a proportion of the value for the standard variety N: Co 310. Any residual effect of the pre-emergence herbicides even at 20 kg/ha a.i. on cane plants was found to be small and insignificant after ploughing field ridges 5 months from cane-planting. With diuron, increasing the rate up to 10 kg/ha began to cause a significant reduction of sugar yield per hectare of cane, while atrazine gave such a reduction only with 20 kg/ha. Détermination de la résistance variétale de la canne à sucre à l'éigard du diuron et de l'atrazine appliqués en pré-levée Résumé. Six variétés de canne à sucre, N:Co 310, F146, F148, F152, F153 et F156, ont manifesté dans un essai au champ des differences de résistance au diuron en pré-levée, avec des mesures de sensibilité de 1,00, 1,57, 1,20, 1,60, 11,30 et 8,07; pour l'atrazine en pré-levée, 0,75, 0,97, 0,59, 1,44, 2,42 et 0,78 respectivement. Une résistance relative fut établie en soumettant chaque variétéà sept doses des herbicides en progression géométrique et en calculant les valeurs de F pour des mensurations mensuelles des plantes de canne, durant la saison de croissance, à la fois pour les plantations d'automne et les repousses de cannes après recépage. Des unités dc sensibilité pour chaque variété furent obtenues en transformant chaque valeur moyenne de F en une proportion de la valeur pour la variety standard N:Co 310. L'effet résiduel des herbicides appliqués en pré-levée, même à 20 kg/ha (m.a.) sur les piantes de cannes fut faible et non significatif apres labour des billons à 5 mois de la plantation des cannes. Avec le diuron l'aecroissement de la dose jusqu’à 10 kg/ha provoqua un commencement de réduction significative de rendement en sucre par hectare, alors que l'atrazine provoqua une telle reduction seulement avec 20 kg/ha. Bestimmung der unterschiedlichen Toleranz von Zuckerrohrsorten gegenüber Diuron und Atrazin bei Vorauflaufbehandlung Zusammenfassung. Im Feldversuch wurde gefunden, dass die sechs Zuckerrohrsorten N: Co 310, F 146, F 148. F 152, F 153 und F 156 sich bei Vorauflaufbehandlung in ihrer Toleranz gegenüber Diuron und Atrazin unterscheiden. Die Suszeptibilitätseinheiten waren für Diuron 1,00, 1,57, 1,20, 1,60, 11,30 und 8,07, für Atrazin 0,75, 0,97, 0,59, 1,44, 2,42 und 0,78. Die Ermittlung der relativen Toleranz erfolgte, indem jede Sorte mit sieben Aufwandmengen der Herbizide in geometrischer Reihe behandelt wurde und die F-Werte für die monatlichen Messungen der Zuckerrohrpfianzen während der Vegetationsperiode berechnet wurden. Die Messungen erfolgten sowohl an im Herbst gepflanztem Zuckerrohr als auch an Wurzelschossen. Die Einheiten der Suszeptibilität für jede Sorte wurden durch Umformung eines jeden mittleren F-Wertes in einen auf die Standardsorte N: Co 310 bezogenen Relativwert erhalten. Die Wirkung von Rücks-tänden der Vorauflaufherbizide auf die Zuckerrohrpflanzen war selbst bei 20 kg/ha Wirkstoff nach Furchenpflügen 5 Monate nach der Auspflanzung gering und nicht signifikant. Diuron begann eine signifikante Minderung des Zuckerertrages pro Hektar hervorzurufen, wenn die Aufwandmenge bis auf 10 kg/ha gesteigert wurde, wahrend Atrazin erst bei 20 kg/ha eine solche Ertragsminderung verursachte.     

实验室人工感染诱发猪瘟野毒垂直传播

利用2头人工感染中等毒力猪瘟野毒耐过猪进行配种,诱发了猪瘟野毒垂直传播.带毒母猪于带毒后171 d产下9头仔猪,其中3头为死胎,6头为木乃伊.直接免疫荧光抗体试验和RT-PCR检测,9头均为阳性.测序结果表明,3头测序仔猪中,2头仔猪所分离病毒E2基因主要抗原编码区序列与公猪所接种病毒的一致;另1头仔猪所分离病毒E2基因主要抗原编码区序列与公猪所接种病毒的同源性高于与母猪所接种病毒的同源性.母猪在与公猪配种前后,其所分离病毒E2基因主要抗原编码区序列发生了变化,配种后与公猪所分离病毒的一致,说明猪瘟病毒在猪体内的繁殖存在一定的优势选择现象.     

新扬州鸡早期增重的OPAY02-SCAR分子标记研究

OPAY02型2条多态性条带经克隆、测序和引物设计后,转换成SCAR标记,并对86个新扬州鸡随机交配后代基因组DNA进行了PCR扩增.2条带DNA序列与红色原鸡基因组序列比对结果表明,大分子量条带与位于红色原鸡第3号染色体上序列有98%的同源性,共检测到8个SNPS,其中195位的碱基T→G,316位的A→T,538位的G→A,731位的T→A,1 147位的G→A,1 329位的T→C,1 927位的C→T,2 081位的C→T,小分子量条带与红色原鸡没有同源序列,推测新扬州鸡野祖除红色原鸡外,还有其它来源.SCAR标记分析表明,经条件优化随机扩增的OPAY02型标记稳定、可靠,可用于遗传分析.2条带所在座位群体基因型平衡性测验结果表明,所测新扬州鸡群体处于平衡状态,选择可以打破平衡,有利于动物育种.     

重庆市农民收入问题结构性分析

从农民收入结构角度，对影响重庆市农民收入增加的因素进行分析，以期更为准确地把握重庆市农民收入问题的实质，最后从农业内部和外部两个方面人手提出增加农民收入的几点主要措施。     

正交试验法优化普抗合剂制备工艺

以干浸膏得率和黄芩苷提取率为考核指标,采用正交试验法对普抗合剂水提取醇沉淀制备工艺进行考察.普抗合剂最佳制备工艺方案为加水量10倍,提取2次,每次1 h,55%的乙醇沉淀杂质 .该工艺科学合理,适合于大规模工业生产.     

杜泊羊血液蛋白质多态性分析

采用垂直板高pH值不连续聚丙烯酰胺凝胶电泳对21只杜泊羊的血红蛋白(Hb)、转铁蛋白(Tf)、白蛋白(Alb)、前白蛋白(Pa)和慢α2球蛋白(Sα2)5个基因座的多态性进行了分析。结果表明杜泊羊的Hb、Tf位点存在多态性,Hb位点共检测到HbA和HbB2个等位基因,构成HbAA、HbBB、HbAB3种基因型,其中HbBB和HbB为优势基因型和优势基因;Tf位点共检测到TfDD、TfCC、TfBD、TfCD4种基因型,受TfB、TfC、TfD3个复等位基因控制,其中TfCC、TfDD基因型和TfC、TfD基因为优势基因型和优势基因。试验未在Alb、Pa和Sα2位点检测出多态性,它们均呈现单态。     

Influence of APP17 peptide on the learning,memory in SAMP10 mi ce and oxidative stress in mouse mitochondria in hippocampal neurons

AIM:To observe the influence of APP17-mer peptide on the learning and memory,and oxidative stress in mitochondria in hipp ocampal neurons of senescence accelerated mouse/prone 10 (SAMP10).METHODS:The 4-month-old SAMP 10s were randomly divided into mod el group and APP17-mer peptide group (the model group+APP17-mer peptide).The se nescence accelerated mouse/resistance1 (SAMR1) were used for normal control.The APP17-mer peptide group was injected with APP17-mer peptide subcutaneously in a dose of 0.34 μg/per mouse,three times a week;while the model group and the control group were injected with saline.After 28 weeks,water maze test was use d to evaluate the learning and memory of these mice.Then hydroxy lamine reactio n and thiobarbituric acid reaction were used to assay the activity of superoxid e dismutase (SOD) and contents of malondialdehyde (MDA) in mitochondria in hippo campal neurons,respectively.RESULTS:(1) The water maze test showed that the full-course swi mming time and numbers of error committed in model group were significantly incr eased as compared with the normal control (P<0.05).The results in APP17-me r peptide treated group were the same as that in normal control group.(2) Compa red with the normal control,the activity of SOD decreased in model group (P <0.01),and the contents of MDA increased (P<0.01).APP17-mer partially re versed the increase in MDA and the decrease in SOD.The content of MDA was lower and the activity of SOD was higher in APP17-mer peptide group than those in mod el group (P<0.05).CONCLUSION:The learning and memory deficit in SAMP10 are observ ed,and the balance of free radical generation/free radical scavenger is impai red.APP17-mer peptide has an antioxidant character and corrects the abnormal ch ange of SOD and MDA,which may be related to its protective effect of learning and memory.