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171.
采用垂直板高pH值不连续聚丙烯酰胺凝胶电泳对21只杜泊羊的血红蛋白(Hb)、转铁蛋白(Tf)、白蛋白(Alb)、前白蛋白(Pa)和慢α2球蛋白(Sα2)5个基因座的多态性进行了分析。结果表明杜泊羊的Hb、Tf位点存在多态性,Hb位点共检测到HbA和HbB2个等位基因,构成HbAA、HbBB、HbAB3种基因型,其中HbBB和HbB为优势基因型和优势基因;Tf位点共检测到TfDD、TfCC、TfBD、TfCD4种基因型,受TfB、TfC、TfD3个复等位基因控制,其中TfCC、TfDD基因型和TfC、TfD基因为优势基因型和优势基因。试验未在Alb、Pa和Sα2位点检测出多态性,它们均呈现单态。 相似文献
172.
AIM:To observe the influence of APP17-mer peptide on the learning and memory,and oxidative stress in mitochondria in hipp ocampal neurons of senescence accelerated mouse/prone 10 (SAMP10).METHODS:The 4-month-old SAMP 10s were randomly divided into mod el group and APP17-mer peptide group (the model group+APP17-mer peptide).The se nescence accelerated mouse/resistance1 (SAMR1) were used for normal control.The APP17-mer peptide group was injected with APP17-mer peptide subcutaneously in a dose of 0.34 μg/per mouse,three times a week;while the model group and the control group were injected with saline.After 28 weeks,water maze test was use d to evaluate the learning and memory of these mice.Then hydroxy lamine reactio n and thiobarbituric acid reaction were used to assay the activity of superoxid e dismutase (SOD) and contents of malondialdehyde (MDA) in mitochondria in hippo campal neurons,respectively.RESULTS:(1) The water maze test showed that the full-course swi mming time and numbers of error committed in model group were significantly incr eased as compared with the normal control (P<0.05).The results in APP17-me r peptide treated group were the same as that in normal control group.(2) Compa red with the normal control,the activity of SOD decreased in model group (P <0.01),and the contents of MDA increased (P<0.01).APP17-mer partially re versed the increase in MDA and the decrease in SOD.The content of MDA was lower and the activity of SOD was higher in APP17-mer peptide group than those in mod el group (P<0.05).CONCLUSION:The learning and memory deficit in SAMP10 are observ ed,and the balance of free radical generation/free radical scavenger is impai red.APP17-mer peptide has an antioxidant character and corrects the abnormal ch ange of SOD and MDA,which may be related to its protective effect of learning and memory. 相似文献
173.
不同光质膜对草莓果实品质的影响 总被引:12,自引:1,他引:12
研究了相同光强下不同光质对‘丰香’草莓果实品质的影响。结果表明, 绿膜、中性膜、黄膜、蓝膜、红膜下生长的果实依次越来越红、越来越暗。果实色度值以红膜下的最大, 其次为中性膜处理,黄膜比中性膜处理略小但差异不显著, 绿膜处理最小, 这与不同膜透射的红橙光比例一致。不同膜处理的果实抗坏血酸含量高低与不同膜透射的紫外光和蓝紫光成分比例一致, 与红光/蓝光比值相反。红膜下的草莓产量最高、果实最大、着色最好, 蓝膜下的草莓果实含糖量、可溶性固形物含量、抗坏血酸含量和固酸比均最高, 绿膜下产量最低、果实最小、着色差且含糖量最低。 相似文献
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多效唑对八仙花组培苗营养生长及成花的影响 总被引:6,自引:1,他引:6
以八仙花1、2 a生组织培养苗为试验对象,研究了多效唑不同浓度、不同施药时间、不同施药次数对八仙花组培苗促成栽培中其植株营养生长及成花的影响.结果表明:施用浓度和时间是多效唑抑制八仙花组培苗植株营养生长促进其生殖生长的关键,7月中旬以浓度100~150 mg/L的多效唑溶液对八仙花1、2 a生组织培养苗进行叶面喷施,最有利于其花芽分化,成花率高,且从株型、花形花色等各项指标综合考虑,多效唑对促进八仙花2a生组培苗成花效果更显著.施药次数也影响八仙花组培苗营养生长和成花,连续施药3次对抑制其营养生长促进成花效果最佳. 相似文献
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180.
AIM: Cinnamyl aldehyde (CA) is one alcohol ingredient derived from Cinnamomum cassia,which is widely used in treating chronic skin wound in Chinese medicine with the curative effect of ‘rescuing YANG’.The purpose of the present study was to investigate the expression of c-Fos,c-Myc proteins at different time points in NIH3T3 treated with CA and explore the possible mechanism of promoting cell proliferation by CA.METHODS: MTT assay was used for observing cell proliferation.Expression of c-Fos and c-Myc proteins in NIH3T3 cells were assessed by immunocytochemistry assay.RESULTS: The cell proliferation was promoted obviously when CA concentration was between 8.8×10-2 μg/L and 8.8×10 μg/L.CA at concentration of 5.5 μg/L significantly induced expression of c-Fos,c-Myc proteins at 2-3 h after the stimulation compared with control group (P<0.01).CONCLUSION: CA increases expression of c-Fos and c-Myc proteins,which may be one of mechanisms for CA to promote NIH3T3 cell proliferation. 相似文献