Effect of 3,5-hydroxy-6,7,3’,4’-tetramethoxyflavone isolated from Laggera pterodonta on apoptosis of Hep-2 cells in vitro

AIM: To investigate the effect of 3,5-hydroxy-6,7,3’,4’- tetramethoxyflavone (HTMF) isolated from Laggera pterodonta on Hep-2 cell apoptosis and the underlying mechanism. METHODS: The MTT assay was used to observe the cytotoxicity of HTMF to the normal cells and the inhibitory effect of HTMF on the proliferation of tumor cells. The apoptosis was determined by flow cytometry. Western blotting was used to detect the expression of caspase-3 and caspase-9. RESULTS: HTMF significantly inhibited the growth of Hep-2 cells in dose and time dependent manners. HTMF exhibited weak cytotoxicity to the two normal cell lines Vero and EVC304, while showed low effect of anti-proliferation on HepG2 cells and A549 cells. The increase in apoptosis of Hep-2 cells by HTMF was observed with dose and time dependent manners. Western blotting showed that HTMF time dependently increased the expression of caspase-3 and caspase-9 in Hep-2 cells. CONCLUSION: HTMF has high inhibitory effect on the proliferation of Hep-2 cells by induction of apoptosis in the tumor cells through caspase-9 and caspase-3 activation. However, the cytotoxicity of HTMF to the normal cells is low.     

茭白配方专用肥肥效比较试验

通过与常规施肥比较,研究茭白配方专用肥对茭白生物学性状、产量和品质的影响。试验结果表明:茭白商品性有明显改善,粗脂肪、维生素C、总糖、氨基酸等品质指标增幅达到极显著水平,并可实现增产增效21.96%,节约肥料成本6.53%,利润提高25.98%。在配方专用肥的基础上增施氮肥,不能提高茭白产量,且肥料成本增加,种植效益明显下降。     

一种改进的基因表达数据分类方法

从分类算法和特征基因选择两个方面研究基因表达数据的分类,将传统的Support Vector Machines(SVM)算法和K-nearest neighbor(KNN)算法两者结合成为一种应用于基因表达数据分类的算法,并针对基因表达数据分类数据集“样本少,维数高”的特点,提出了一种改进的基于相关性的递归特征消除算法(简称为C-RFE),消除了数据冗余．实验结果表明,新方法可有效提高分类准确率和特征选取的效率．     

Effects of radix pseudostellariae on cardiac function and matrix metalloproteinases in rat chronic heart failure induced by coronary artery ligation

AIM:To investigate the effects of radix pseudostellariae on cardiac function and matrix metalloproteinases (MMPs) in rat chronic heart failure induced by coronary artery ligation. METHODS:Rat model of chronic heart failure was produced by ligating the coronary artery for 6 weeks. The cardiac functions were determined by observing the hemodynamics parameters. mRNA expressions of MMP-2 and MMP-9 in left ventricular tissue were detected by RT-PCR and activities of MMPs were assayed by zemography.RESULTS:Cardiac function was remarkable turbulence after coronary artery ligation 6 weeks, according to the results of the hemodynamics. The mRNA contents and activities of MMP-2 and MMP-9 in left ventricular tissue were significantly elevated. Radix pseudostellariae remarkable ameliorated the cardiac functions, inhibited the mRNA contents and activities of MMP-2 and MMP-9 in left ventricular tissue of rat with chronic heart failure after continued 5 weeks treated with decoction of radix pseudostellariae. CONCLUSION:The decoction of radix pseudostellariae ameliorates cardiac functions of rat with chronic heart failure. The mechanism maybe involves in the inhibition of the MMP-2 and MMP-9 mRNA expression and activities in cardiac ventricles.     

外出打工对中部农村留守家庭社会适应的影响分析——农村社区关系资源网络代际继替裂痕对家庭的影响

在缺乏外部社会保障有效介入的农村,依靠地缘和血缘关系建立的社区关系资源网络为农村家庭提供着有效的社区保障。随着农村大规模、长期性的外出进城打工,引发家庭主导群体的家庭撤离和社区撤离,使得具有独特个人性和代际继替特征的社区关系资源网出现代际继替裂痕,这对农村家庭的社区支持将产生重要影响。从社区关系资源网代际继替的角度浅析外出打工对农村家庭生活的影响。     

家兔原始卵泡卵母细胞体外发育的培养体系研究

本研究根据家兔卵巢的发育特征初步探讨了家兔卵巢卵母细胞体外发育的培养体系。采集新生或30日龄家兔卵巢在不同培养液中培养,并分析家兔卵母细胞的体外发生与成熟情况与体内正常发育的兔卵巢卵母细胞之间的差异,来筛选和优化家兔卵巢卵母细胞的体外培养体系。本试验从高糖DMEM、FSH浓度、BSA/血清等3个方面来筛选较好的培养条件,结果表明:①不添加高糖DMEM的M199培养液与添加的相比,卵巢上卵母细胞数量要多,但随着培养时间的延长,组织块上卵母细胞的形态会变得不清楚;②家兔卵巢组织在FSH浓度为50和100m IU/m l、添加血清的DMEM/F12培养液中生长较好,体外培养24d后,卵母细胞数量多,直径增加到65μm;③与添加BSA的培养液相比,添加血清的培养液中的卵巢卵母细胞生长状态较好,体外培养28d后,卵母细胞数量多,并且直径可达65μm。     

浪河水库心墙堆石坝应力变形分析及安全评价

采用非线性有限元法,应用邓肯E-B模型对浪河水库黏土心墙堆石坝进行了应力变形分析.计算模拟了大坝未蓄水时、蓄水后及水位突降过程,得出各个时期的坝体最大断面上各部分的位移和应力分布.计算结果表明,在上游坝坡处堆石部分有滑坡的可能性,采用刚体极限平衡法给予校核,计算结果与实测规律一致.对于坝体渗流稳定也做了相应的计算分析,并对坝体安全稳定作出了相应的评价.通过分析得知坝体裂缝主要是坝体产生不均匀变形所致,分析结果为坝体加固措施的设计及实施提供了必要的参考.     

Closantel缓释剂对实验感染肝片吸虫山羊机体自由基影响的研究

选用5只实验感染肝片吸虫山羊研究Closantel缓释剂对机体抗氧化功能的影响，实验用山羊每只一次性口服肝片吸虫囊蚴150个，感染后第15周随机将山羊分成对照组（n=2）和驱虫组（n=3），驱虫组每羊口服两颗Closantel缓释剂，每周定时采集颈静脉血液，检测血清抗氧化酶的变化，观察服用Closantel缓释剂对其的影响。结果表明投药后山羊血清中GSH－Px、CAT均有不同程度的升高，SOD变化不显著，而脂质过氧化产物MDA含量则下降，提示Closantel的缓释剂型对实验感染肝片吸虫山羊机体的抗氧化产生了影响，对机体有一定保护作用。     

论退耕还林的政策缺失及对策措施

退耕还林工程实施7年来,国家采取了一系列有力措施,工程建设取得了阶段性成果。但现行政策仍然存在着诸多缺失,由此引发了退耕还林的诸多问题。文章从公共政策的视角出发,对现行政策及其缺失进行了系统的分析,并提出了政策调整完善的对策建议。