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31.
Detection of arboviruses in Culicoides(Diptera: Ceratopogonidae) collected from animal farms in the border areas of Yunnan Province,China 下载免费PDF全文
DI Di LI Chen-xi LI Zong-jie WANG Xin XIA Qi-qi Mona SHARMA LI Bei-bei LIU Ke SHAO Dong-hua QIU Ya-feng Soe-Soe WAI YANG Shi-biao WEI Jian-chao MA Zhi-yong 《农业科学学报》2021,20(9):2491-2501
Biting midges of the genus Culicoides(order Diptera, family Ceratopogonidae) are potential biological vectors for the transmission of certain arboviruses among humans, livestock, and wild animals. This study collected a total of 405 Culicoides individuals from seven animal farms located in five counties in the border areas of Yunnan Province, China, and examined the Culicoides species composition and the major arboviruses carried by the Culicoides species. The collected Culicoides were classified into seven species with variable abundances: Culicoides arakawae(5.43%, 22/405), Culicoides homotomus(1.23%, 5/405), Culicoides obsoletus(19.75%, 80/405), Culicoides orientalis(17.28%, 70/405), Culicoides oxystoma(29.38%, 119/405), Culicoides peregrinus(5.68%, 23/405), and Culicoides nipponensis(21.23%, 86/405). Among the seven species, C. oxystoma and C. nipponensis were distributed in all the five counties with abundances of 13.33–44.87% and 10.00–46.83%, respectively, suggesting that these were the dominant species of Culicoides widespread on animal farms in the border areas. PCR was used to detect major arboviruses in the collected Culicoides specimens, including bluetongue virus(BTV), Japanese encephalitis virus, Dengue virus, Zika virus, African swine fever virus, and African horse sickness virus. Among the tested viruses, only BTV serotype 1 was tested positive in C. oxystoma specimens collected from a buffalo farm. Culicoides oxystoma was the dominant species on animal farms in the sampled areas, but it has not previously been documented as positive for BTV in China. The current results thus suggest that C. oxystoma could be an important vector for BTV transmission in these border areas, which, however, needs to be confirmed by further comprehensive experiments. Overall, the present study provides the first profile of Culicoides species on animal farms in the China, Vietnam, and Myanmar border areas, establishes the prevalence of arboviruses carried by these Culicoides species, and suggests the vector potential of C. oxystoma species for the transmission of BTV. 相似文献
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为了探究新型栽培方式下甜椒苗期N20的排放量和排放昼夜变化规律,明确新型栽培方式下最佳的蔬菜减排栽培模式,通过静态箱-气相色谱法分别对土垄栽培、土垄内嵌式基质栽培(SSC)标准垄、SSC矮垄3种栽培模式的内嵌区和整个垄部连续取气测定N2O排放量.结果表明,测定时间段内,设施甜椒苗期的N2O排放通量出现两个排放峰值,呈现... 相似文献
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农杆菌介导的hIL-12基因转化马铃薯的研究 总被引:3,自引:1,他引:2
构建CaMv35S启动子驱动人白细胞介素12基因(hIL-12)的植物表达载体pBI121-hIL-12,通过三亲杂交法将重组载体导入根癌农杆菌菌株EHA105,农杆菌介导法转化马铃薯茎段,经卡那霉素抗性筛选,获得28株抗性植株。通过PCR检测,22株为阳性,从PCR阳性植株中随机选取5株,ELISA法检测茎和叶中hIL-12的表达量,其中2株与对照组相比有显著性差异(p<0.001),hIL-12的表达量分别为(3 714.0±48.8)pg/g和(3 465.0±185.0)pg/g,初步表明外源基因hIL-12已经整合进马铃薯基因组中并得到了表达,为进一步研究重组hIL-12的分离纯化和生物学活性奠定了试验基础。 相似文献
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AIM: To investigate the effect of antisense oligodeoxynucleotides (AS-ODN) on the intercellsular adhesion molecule-1(ICAM-1) expression on endothelial cells in hypoxia/reoxygenation(H/R). METHODS: With cultured glomerular vascular endothelial cell in H/R, the positive percentage of ICAM-1 expression was measured by flow cytometry before and after giving AS-ODN. RESULTS: The ICAM-1 expression did not increase on glomerular vascular endothelial cell in 10 hours hypoxia compared to control group, it increased in 6 hours reoxygenation, and decreased by 40.6% after giving AS-OND. CONCLUSION: AS-ODN may decrease the expression of ICAM-1 on endothelial cells in H/R. 相似文献
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植物秸秆纤维素物理化学改性及其吸附机理研究 总被引:3,自引:1,他引:2
采用植物秸秆为原料,借助高压蒸汽闪爆技术、稀碱蒸煮等方法得到具有一定α-纤维素含量的秸秆基纤维素,在此基础上进行碱化、醚化和胺基亲核取代反应,制备出对Cu^2+和Cd^2+等重金属离子具有优良吸附性能的乙二胺螯合植物秸秆纤维素,并对其吸附机理进行研究。结果表明,用蒸汽闪爆物理方法可以纯化天然植物秸秆纤维素,制备的吸附材料对金属铜离子、镉离子吸附规律基本符合Freundlish等温吸附式,其等温式中lgC系数分别为0.997和1.159。 相似文献
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热空气处理对红富士苹果贮藏品质及其生理指标的影响 总被引:2,自引:0,他引:2
采用38℃72h和96h热空气处理红富士苹果,经过16周0℃的冷藏期后,置于货架条件1周。试验结果表明,冷藏期间,与对照组(未经热处理)相比,处理组果实果皮叶绿素含量较低,呼吸强度和乙烯释放量保持较低水平;果实硬度较高,水溶性果胶低,而非水溶性果胶含量较高;可溶性蛋白和热稳定蛋白含量下降缓慢,热稳定蛋白含量在可溶性蛋白中所占比例较高。热空气处理可以较好地保持果肉细胞膜的稳定性,使果实货架期果面底色较黄,固酸比上升,果汁pH值较高,具有较好的外观品质。 相似文献