当代大学生网络迷恋的心理特征分析——以信阳农业高等专科学校2006级新生为例

热衷于网络游戏和聊天交友是大学新生迷恋网络的主要原因,也是他们缺乏明确的学习、生活及奋斗目标的体现。学校和社会应共同努力,引导大学生们理性上网。     

混凝土外加剂对混凝土的影响

介绍混凝土外加剂的概念和作用,针对目前传统混凝土、水泥砂浆在使用过程中常因塑性及干缩等原因产生开裂现象,提出在混凝土施工过程中适当加入混凝土外加剂有助于提高混凝土的强度和密实性,改善混凝土拌合物和易性,节约水泥,防止混凝土受冻破坏。     

新农村小城镇和城市生活垃圾收集清运中转设备

介绍了全国各地新农村小城镇及城市生活垃圾清运的各种方式,指出了我国城市生活垃圾清运设备存在的弊端,开发设计了一种将散装垃圾压缩成块储存一块再压一块,两块垃圾一起推入垃圾车辆的成套装置,解决了散装垃圾运输对城市环境的二次污染,实现了汽车满载运输不亏吨的目的,提高了运输效率,节能降耗环保.     

壳聚糖诱导黄瓜幼苗抗灰霉病

为了明确壳聚糖(chitosan,CTS)对黄瓜幼苗抗灰霉病的诱导作用,采用根际注射结合叶面喷洒的方法,测定了灰霉病菌侵染下幼苗植株病情指数、防御酶活性和抗病相关物质含量等生理指标。结果表明:CTS降低了黄瓜幼苗的病情指数,最高幅度达39.4%,提高了苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)活性,增加了总酚、类黄酮和木质素含量。说明CTS能诱导黄瓜幼苗对灰霉病的抗性,且最佳处理浓度为200mg·L-1。     

Eukaryotic expression of human Arresten gene and its effect on the proliferation and migration of vascular smooth muscle cells

AIM: To express human Arresten gene in eukaryotic cell,and to investigate its effect on the proliferation and migration in vitro of rat primary cultured thoracic aortic vascular smooth cells (VSMCs).METHODS: COS-7 cells were transfected with recombinant eukaryotic expression plasmid pSecTag2-AT or control plasmid pSecTag2 mediated by liposome.48 hours after transfection,polymerase chain reaction (RT-PCR) was used to detect the expression of Arresten mRNA in the cells,while Western blotting assay was applied to detect expressed Arresten protein in concentrated supernatants.VSMCs were then co-cultured with the concentrated supernatants;and its proliferation was detected using cell counting kit-8 (CCK-8) in vitro.Migration of VSMCs was assayed by a microchemotaxis chamber and a polycarbonate filter (Transwell's chamber) with pores of 8 μm in diameter.RESULTS: RT-PCR revealed that the genome of Arresten-transferred cells contained a 449bp specific fragment of Arresten gene.Successful protein expression in supernatants was confirmed by Western blotting.CCK-8 assay showed that the proliferation of VSMCs was inhibited significantly by Arresten protein as compared with control group (P<0.01).Transwell's chamber showed that the number of control group,pSecTag2 transfected group and pSecTag2-AT transfected group were 28.70±3.97,26.10±4.53 and 14.00±3.33 (P<0.01).CONCLUSION: Arresten protein expressed in eukaryotic cells inhibits the proliferation and migration of VSMCs effectively in vitro.     

重组猪瘟病毒E2基因逆转录病毒载体的构建及其表达活性

从实验感染兔脾组织中提取总RNA，应用RT—PCR得到了CSFVC-株结构蛋白E2基因，定向克隆于逆转录病毒载体pBABEpuro，经PCR、酶切和序列分析鉴定，获取阳性重组质粒。将该重组质粒与水疱性口炎病毒载pVSV—G共转染GP2—293细胞，收获假型病毒，并在Polybrene的介导下感染PK15细胞，嘌呤霉素筛选表达猪瘟E2基因的细胞株。通过细胞传代并结合PCR、免疫荧光及ELISA检测表明，所筛的细胞细胞系能稳定表达猪瘟E2基因，而且表达产物具有良好的生物学活性。     

工厂化栽培杏鲍菇优良菌株筛选

杏鲍菇(Pleurotus eryngii)又名刺芹侧耳,隶属于担子菌亚门(Basidiomycotina),层菌纲(Hymenomycetes),无隔担子菌亚纲(Homobasidio-mycetidae),伞菌目(Agaricales),侧耳科(Pleuro-taceae),侧耳属(Pleurotus)。杏鲍菇菇柄色泽雪白,质地脆嫩,素有“平菇王”的美誉[1]。杏鲍菇人工     

微波消解-石墨炉原子吸收光谱法测定醋酸地塞米松中的微量硒

采用微波制样技术对样品进行消解,减少了消化过程中硒的损失,利用石墨炉原子吸收光谱法对醋酸地塞米松中微量硒进行测定.以4 mg/mL Pd(NO3)2作为基体改进剂,灰化温度为950 ℃,原子化温度为2 100 ℃时,经氘灯校正背景,测定硒含量,回收率在89.24%～112.37% 之间,方法的相对标准偏差为7.42%,检出限为0.53 ng/mL.该法具有简便、快速、灵敏、稳定、准确等优点,适用于醋酸地塞米松中微量硒的分析测定.     

金丝桃素提取、分离方法的研究

目的:研究贯叶连翘中有效成分金丝桃素提取方法。方法:应用有机溶剂溶解法、薄层色谱法、紫外检测、高效液相色谱(HPLC)等方法探讨贯叶连翘中金丝桃素提取方法。结果:用石油醚除叶绿素、花青素,以丙酮为溶剂在60℃水浴中提取贯叶连翘中金丝桃素和酮类衍生物,在真空度为0.07MPa、温度为60℃水浴条件下浓缩。后用100-200目硅胶分离金丝桃素,钢瓶冻干,得含金丝桃素为1.08%深红色产品。