单宁酸对猪卵母细胞体外成熟及胚胎发育能力的影响

研究旨在探讨单宁酸对猪卵母细胞体外成熟质量及其胚胎发育能力的影响。在猪卵丘卵母细胞复合体（COCs）体外成熟培养液中添加不同浓度（0、1、10、100 μg/mL）单宁酸培养42 h后,检测COCs的扩散程度和卵丘细胞扩散指数,统计COCs的体外成熟率,检测成熟卵母细胞内谷胱甘肽（glutathione,GSH）、活性氧（reactive oxygen species,ROS）和生长分化因子9（growth differentiation factor 9,GDF9）的水平,并统计孤雌激活及体外受精胚胎48和168 h的卵裂率、囊胚率及囊胚总细胞数。结果显示,与对照组相比,10 μg/mL单宁酸组卵丘细胞扩散指数显著提高（P<0.05）,100 μg/mL单宁酸组显著降低（P<0.05）;1和10 μg/mL单宁酸组卵母细胞成熟率差异不显著（P>0.05）,100 μg/mL单宁酸组卵母细胞成熟率显著降低（P<0.05）;1和10 μg/mL单宁酸组GSH和GDF9水平显著提高（P<0.05）,ROS水平显著降低（P<0.05）。孤雌胚胎和体外受精胚胎发育能力结果显示,与对照组相比,各单宁酸组卵裂率差异不显著（P>0.05）,10 μg/mL单宁酸组孤雌胚胎囊胚率及体外受精胚胎囊胚率显著提高（P<0.05）,100 μg/mL单宁酸组孤雌胚胎囊胚细胞数及体外受精胚胎囊胚细胞数均显著低于其他各组（P<0.05）。以上结果表明,10 μg/mL单宁酸可通过提高卵丘细胞扩散能力及GSH和GDF9水平、降低卵母细胞内ROS水平,改善猪卵母细胞成熟质量,提高孤雌胚胎及体外受精胚胎的发育能力。     

现浇混凝土衬砌渠道的施工工艺及流程

主要介绍混凝土衬砌渠道的施工方法、步骤及新工艺的运用;聚苯乙烯泡沫塑料板(简称苯板)防冻胀措施的采用,避免了混凝土衬砌板的冻胀破坏,大大延长了渠道的使用寿命;聚胺脂苯板伸缩缝的设置,由于在混凝土伸缩缝中镶嵌了弹性圆柱条,使聚胺脂的粘合性和延展特性得到充分发挥,既保证了混凝土板有足够的伸缩变形,又避免了聚胺脂的撕裂,减少了渠道水的渗漏损失.     

影响‘小凤兰’根状茎增殖、分化和壮苗的因素研究

以‘小凤兰’根状茎为试材,研究了影响‘小凤兰’根状茎增殖、分化、生根壮苗的因素。结果表明:MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.5mg/L、活性炭0.5g/L有利于根状茎增殖,而添加20%椰汁和番茄汁40g/L会抑制根状茎增殖。1/2MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.2mg/L、20%椰汁、1 000lx光照强度有利于根状茎分化,不加活性炭有利于芽分化,但抑制根分化,而添加活性炭则抑制芽分化,促进苗分化。添加土豆泥60g/L显著促进试管苗生长,3~4cm高的苗在培养基1/2MS+6-BA 0.1mg/L+NAA0.5mg/L+蔗糖20g/L+土豆泥60g/L+卡拉粉8g/L+活性炭0.5g/L中培养40d即可移栽。     

不同基因型大麦苗期对干旱胁迫的响应及HvPIPs基因表达特征分析

为研究不同基因型大麦苗期对干旱胁迫的响应,挖掘优质抗旱的大麦种质资源,以4份不同基因型大麦(2个高抗品种ZDM5430和ZDM5458,2个干旱敏感型品种7DCADA和IL-12)为材料,利用聚乙二醇(PEG-6000)模拟干旱条件,综合分析不同品种大麦的苗期抗旱性差异;并通过对不同品种根系进行PIPs实时定量分析,研究水通道蛋白AQPs基因与干旱胁迫之间的应答关系。结果表明,持续干旱胁迫会使大麦生长变缓,各处理苗长、根长、叶含水量和根含水量都呈下降趋势,其中,4个品种的大麦叶含水量在干旱胁迫14 d时下降最为显著,与对照相比,其降幅分别为29.14%、52.64%、21.67%和72.15%。干旱胁迫对大麦苗期干物质积累量和根冠比的影响较小,各处理间差异不显著;但持续干旱后,抗旱品种ZDM5430的根冠比呈上升趋势,在胁迫7、14、21 d时较CK分别增加了4.92%、42.86%和21.05%。随着胁迫时间的延长,大麦旗叶叶绿素含量和根系活力也呈下降趋势,其中ZDM5430和ZDM5458较对照组降幅较小,说明抗旱品种的形态指标、叶绿素含量及根系活力受干旱胁迫的影响较小。通过PIP...     

A Furry Synthetic Evaluation Model in Capital Structure Decisions

This paper analyzes the limitation of regarding the weighted average cost of capital as the optimal standard of capital structure in the traditional capital structure decision-making method, and analyzes the view that the capital structure is affected by many factors,such as earning power, long-term stability of operating, the attitudes held by owners and operators, the attitudes held by creditors and financial risk. With the example, the affecting factors of the capital structure is analyzed. The fuzzy synthetic evaluation model are employed to decision-making of the capital structure. This article also shows the key point of its application.     

Knowledge-based Intelligent Decision-making System for the Safety of Rockets

With the progress of science and technology, the astronautics has obtained great development and researched in many countries, the problem on rocket safety control is important during the rocket launching proceeding, and is also the focus of every space country. At first, the importance of rocket safety control is introduced and the characteristics of the existing system for the safety of rockets are analyzed. After indicating its disadvantages, the design idea, system framework and method for realization of the knowledge-based intelligent decision-making system are proposed to improve certainty and authenticity of safety decision, containing correspond knowledge and adopting the technology of intelligent decision and reasoning on AI.     

Design of the PC/104 Bus Mode Multi-channel Analyzer

This paper expatiates on the bus structure and develop the bus mode Multi-Channel Analyzer (MCA). The nucleus of this MCA is the PC/104 embedded computer, and it introduces the bus communication to realize system and data communication. It may realize data collecting and showing in the field, because the bus mode is rapid. At first, on the hardware we introduce CMOS components, this reduces power, volume and weight. These make it adapts to use in the field; besides, on the software we adopt C and assembly language to program in order to improve conversion speed. At the same time, single-task DOS operating system is infroduced, which makes the software run more steadily, operate more simply. By means of testing on the spot it can normally work, so it suitable to work in the field.     

Accomplishment of Elastic-plastic Analysis in Geotechnical FEA

In order to realize elastic-plastic analysis in geotechnical FEA, in this paper, firstly, the method how to realize elastic-plastic analysis in Super-Sap is discussed. Then, this paper stresses to introduce the principle and the steps used to achieve the accomplishment of elastic-plastic analysis in Super-Sap. Finally, an engineering practice is given to prove that the geotechnical FEA elastic-plastic analysis and visualized post-processing can come true by it rapidly.