In vitro differentiation of exocrine pancreatic cells from mouse embryonic stem cells

AIM: To explore the effects of sodium butyrate, activin A and dexamthasone on inducing mouse embryonic stem (ES) cells to differentiate into exocrine pancreatic cells in vitro. METHODS: E14 mouse ES cells were cultured in suspension to form embryonic bodies (EBs). The EBs were cultured with differentiating medium containing different concentrations of sodium butyrate, and the spontaneously differentiated ES cells were used as control. Exocrine pancreatic genes such as amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected by RT-PCR at different time points to determine the optimal concentration and exposure time of sodium butyrate. Furthermore, activin A or dexamthasone was also used to explore the effects on exocrine differentiation. After that, the combination of sodium butyrate, activin A and dexamthasone was used to promote the differentiation of exocrine pancreatic cells from ES cells. During the differentiation course, the gene expressions of amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected by RT-PCR. Morphological changes were investigated by phase contrast microscopy. Amylase expression was examined by immunofluorescence staining. RESULTS: Exocrine pancreatic gene expressions such as amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected in spontaneously differentiated EBs. A relatively lower concentration of sodium butyrate with a shorter exposure time significantly promoted those above gene expressions as compared to that of spontaneously differentiated EBs. Activin A and dexamethasone induced upregulation of exocrine gene expression. The combination of activin A, sodium butyrate and dexamethasone significantly enhanced the mRNA levels of amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase. Under the treatment of activin A, sodium butyrate and dexamethasone, differentiated cells were polygonal in shape with large, round, and center-situated nuclei. According to the observation of immunofluorescence staining, amylase was positive expressed at the final stage. CONCLUSION: These data indicate that exocrine pancreatic differentiation of ES cells is induced by sodium butyrate, activin A and dexamethasone. The combination of pancreatic inducing factors improves the differentiating efficiency.     

黄连、黄芩提取液与二氧化氯复配对结球甘蓝种传黑斑病的抑菌效果研究

采用三元二次回归正交旋转组合设计方法,确立了黄连、黄芩提取液与二氧化氯对甘蓝链格孢菌抑制效果最佳的复配组合:黄连提取液13.48%、黄芩提取液15.56%、二氧化氯0.14%.用此复配剂浸种,芸苔链格孢菌和甘蓝链格孢菌的侵染率分别降低了97.33%和94.85%,且提高了种子发芽势及萌发相关酶活性.     

山东省沙地葡萄茎痘相关病毒的检测

为明确沙地葡萄茎痘相关病毒(GRSPaV)在山东省发生的情况,2006-2007年在葡萄主栽区和葡萄品种资源圃进行了田间调查、样品采集和病原检测。应用GRSPaV外壳蛋白基因表达获得的多克隆抗体,经间接酶联免疫吸附试验(PTA-ELISA)和斑点酶联免疫吸附试验(Dot-ELISA)检测,在2a采集的298个样品中,125个样品为阳性反应,阳性率41.95%。125个阳性样品涉及43个品种,品种被侵染率74.14%。研究对检测的样品用RT-PCR法验证,有3对引物均扩增出了预期片段,分别为解旋酶基因340bp片段、外壳蛋白(CP)基因842bp片段和RNA聚合酶(RdRp)基因499bp片段。     

东方红-YZDZ80C液压自行式移动电站

介绍东方红-YZDZ80C液压自行式移动电站的主要技术参数和主要部件结构性能特点。     

论服饰广告的审美创作

服饰广告的本体功能是促进服饰商品的销售。设计师借助美学手段进行审美创作,能巧妙地融服饰广告的经济价值于审美价值之中,从而达到吸引受众眼球,提升服饰商品的知名度、美誉度,实现销售商品的终极目的。从美学的角度对服饰广告创意的表现手法进行审视,有助于指导具体的广告创作实践,提升服饰广告的审美价值,实现美学与服饰广告的完美结合。     

灌溉水矿化度及盐分带入量对小麦相对产量影响的统计分析

在搜集、统计大量咸水灌溉试验资料的基础上,选取咸水充分灌溉条件下矿化度和小麦产量的完整对应数据103组,统计分析了灌溉水矿化度及单位面积盐分带入量对小麦相对产量的影响,结果表明,随着灌溉水矿化度的增加,小麦相对产量呈线性递减趋势,当矿化度大于1.0 g/L时,灌溉水矿化度每增加1 g/L,小麦减产约5.6%;与矿化度类似,随着单位面积盐分带入量的增加,小麦的相对产量也线性降低,盐分带入量每增加1000 kg/hm2,小麦的产量将减少约2%。进一步的检验结果显示,应用灌溉水矿化度及单位面积盐分带入量均可较为可靠地估算小麦的相对产量,估算相对误差基本可控制在16%以内。     

阿月浑子嫁接技术

阿月浑子 ( Pistacia vera L.)商品名称“开心果”,是世界著名四大坚果之一。新疆喀什自二十世纪二十年代中从乌兹别克引进试种栽培 ,但由于没有良种 ,无性繁殖技术难题未解决 ,栽培上存在着结实晚、产量低、坚果品质差等问题。制约了阿月浑子生产发展。新疆林科院、喀什地区林科所阿月浑子课题组自 1994年起 ,在良种引育基础上进行了以阿月浑子为本砧的嫁接技术研究 ,累积嫁接 30 0余株。成活率稳定在 80 % ,嫁接树 3～ 4年挂果 ,株产量提高 1倍 ,坚果品质提高 2～ 3个等级。现将阿月浑子嫁接技术介绍如下 :1　树体特性阿月浑子树系漆树科…     

不同小麦品种搭配生产兰州拉面专用粉的灰色关联度分析

为研究配麦粉制作兰州拉面的特性,将强筋小麦品种甘春20号与中筋小麦品种宁春18号或弱筋小麦品种高台麦分别以11种比例搭配成22份配麦粉,测定了其面粉理化品质及面团品质,并用灰色关联度分析法对所有配麦粉的兰州拉面品质做了初步分析.结果发现,所有配麦粉中拉面品质位居前5名的依次是甘春20∶宁春18=2∶8,4∶6,5∶5,3∶7,0∶10;甘春20号占100%或高台麦比例较高的配麦粉,品质名序都很靠后.这说明,较低比例的强筋小麦与较高比例的中筋小麦搭配的面粉适合做兰州拉面,单纯的高筋力和弱筋力小麦粉都不适合做拉面.     

荔枝低位截干换种试验初报

荔枝生产中,种植品种选择不当或树形培养差,导致树高密蔽,不便管理,容易造成病虫害发生、产量低,效益不高,影响种植者的积极性。对失管荔枝果园、密闭荔枝果园采取低位截干换种技术,有利于快速形成树冠,早结丰产,提高种植荔枝的经济效益。     

山东省玉米病毒病病原鉴定与防治研究

利用基因组核苷酸全序列测定、电镜观察、血清学测定、传播介体传毒试验等方法对山东省田间发生的玉米病毒病进行了病原鉴定。结果表明,山东省玉米病毒病病原主要是甘蔗花叶病毒山东株系(SCMV-SD)和玉米粗缩病毒(MDMV)。田间试验5种防治植物病毒病专用药剂对玉米病毒病均有一定的防病效果,最高防效在60%左右。采用种植抗病品种、苗期拔除病苗、药剂拌种防虫和适期施用农药等综合防治措施,防病效果达61.35%～85.75%,增产率6.25%～13.99%。