Clinical significance of immunohistochemically detecting forkhead box P3 expression in human astrocytic tumors

AIM: To investigate the expression of forkhead box p3 (FOXP3) protein in astrocytic tumors, and analyze its clinical significance.METHODS: We measured the incidence of FOXP3 in 121 astrocytomas, 5 meningiomas, 5 normal brain tissues using immunohistochemistry, analyzed the correlation of the presence of FOXP3 with histopathologic features and survival. RESULTS: FOXP3 exists in human brain astrocytic tumors, which was not found in meningioma and normal brain tissues. FOXP3 was expressed in interstitial lymphocytes mainly, and expressed in tumor cells rarely. The differences on FOXP3 expression between all grade levels were statistically significant (P<0.05). The Kaplan-Meier survival analysis showed that there was significant difference between the two survival curves, and the survival rate of the group with negative FOXP3 expression was higher than that of the positive group. Multivariate Cox proportional hazards regression analysis indicated that FOXP3 could not serve as an independent prognostic factor of astrocytic tumors survival time (P＞0.05).CONCLUSION: FOXP3 immunoreactivity is significantly associated with the age and the malignancy of human astrocytic tumors. Astrocytic tumor patients with FOXP3 expression have poorer prognosis, while FOXP3 can not serve as an independent prognostic factor of astrocytic tumors survival time. FOXP3 may provide a new target for immunotherapy of astrocytic tumors.     

人工饲喂芽孢杆菌的哈萨克羔羊肠道形态结构研究

将24只3月龄的新疆哈萨克羔羊,随机分为2组,对照组饲喂基础日粮并口服生理盐水;试验组羔羊口服108 CFU以上的芽孢杆菌HS06菌液.饲喂14 d后采样测定肠道的绒毛高度、黏膜厚度、肌层厚度以及IL-6的水平.结果与对照组相比,试验组羔羊肠道不同肠段绒毛长度、黏膜厚度及肌层厚度皆显著增加(P<0.05),并且显著提高...     

Clinical significance of 4-1BBL protein expression in human glioma tissues

AIM: To investigate the expression of 4-1BBL(CD137L) protein in human glioma tissues, and to analyze its clinical significance. METHODS: The expression levels of 4-1BBL protein in 82 human glioma specimens were measured by the method of immunohistochemistry. The correlation of 4-1BBL protein level with histopathologic features and survival time was analyzed. RESULTS: 4-1BBL protein positive cells were observed in human brain astrocytic tumors, which were not found in meningioma and normal brain tissues. In our study, the expression of 4-1BBL protein in 3 cases of normal brain tissues was negative. The expression of 4-1BBL protein in 31 cases of glioma specimens were positive, and total positive rate was 37.8% (31/82). The expression of 4-1BBL protein was related to the survival time and age of the patients, but not related to the pathological grade of glioma. Statistically, the patients with positive expression of 4-1BBL protein had longer survival time (P<0.05). CONCLUSION: Expression of 4-1BBL protein may have reference value to predict the prognosis of patients with glioma.     

SNP芯片评估柯尔克孜羊群体遗传多样性和遗传结构

旨在了解柯尔克孜羊群体的遗传多样性和遗传结构,有效地保护和利用其遗传资源。本研究利用绵羊SNP 50K v3芯片检测61只柯尔克孜种羊(31只公羊、30只母羊)个体的单核苷酸多态性(single nucleotide polymorphism, SNP);Plink(V1.90)软件对数据进行质控,计算群体有效含量、多态标记的比例、观测杂合度、期望杂合度、多态信息含量、有效等位基因数、最小等位基因频率,分析群体的遗传多样性;Plink计算连续性纯合片段(runs of homozygosity, ROH)和近交系数F_ROH;构建状态同源距离矩阵(identical by state, IBS),并采用Gmatrix软件构建G矩阵,解析柯尔克孜羊群的遗传距离和亲缘关系;使用Mega X软件构建种公羊进化树,分析群体家系结构。结果显示,61只柯尔克孜羊共得到64 734个SNPs标记,通过质检的SNPs为56 763个;平均多态信息含量为0.273±0.112,平均观察杂合度和平均期望杂合度分别为0.368±0.140和0.368±0.130,平均最小等位基因频率为0...     

猪产肠毒素大肠杆菌_（987）P菌毛卵黄抗体的研究

【目的】探索产肠毒素大肠杆菌（ETEC）987P菌株培养的最佳条件及987P菌毛诱发蛋鸡制备卵黄抗体（IgY）的最佳免疫程序,为IgY的工业化、规模化生产提供参考。【方法】扩大培养987P菌株,抽提层析纯化987P菌毛蛋白,然后以其为抗原免疫20周龄开产海兰蛋鸡,采用间接ELISA检测987P菌毛蛋白免疫IgY效价。【结果】987P菌株的最佳培养条件为37℃下Slanctz固体培养基培养24h;以纯化987P菌毛蛋白为抗原免疫20周龄开产蛋鸡,发现987P菌毛蛋白二免、三免IgY效价总体趋势基本一致,均在第3周达到峰值并维持1周,二免抗体峰值为213,三免抗体峰值是二免峰值的2倍（214）,但第4周过后三免IgY效价仍保持在213,且持续时间长,一般维持2～3周。【结论】以纯化987P菌毛蛋白为抗原免疫蛋鸡,能获得较高效价的IgY,且适当增加免疫次数能有效提高抗体水平和延长持续时间。     

不同土地利用方式对藏东南典型草原土壤真菌群落的影响

探究不同土地利用方式对土壤真菌群落的影响可为藏东南典型草原合理利用提供理论依据。本试验选取青稞农田(HC)、垂穗披碱草人工草地(EAG)和围封草地(EG)为研究对象,以未开垦天然草地(NG)为对照,采用高通量测序技术分析真菌群落特征变化。结果表明：与对照相比,HC、EAG和EG样地土壤有机质、全氮、硝态氮和速效磷含量显著升高;HC和EAG样地真菌群落香农指数显著降低。各样地差异标志物种数量变化排序为HC(9个)>EAG(6个)=EG(6个)>NG(4个)。土壤容重、pH值、有机质和全氮含量与真菌群落多样性变化密切相关。有机质、全氮、铵态氮、速效钾和pH值是真菌门水平组成的主要影响因子;属水平为土壤pH值、铵态氮、容重、有机质和全氮含量。因此,草原开垦为农田和人工草地后长期利用有利于土壤养分含量提升,围封技术适合高寒草地生态保护与利用。     

补饲对母牦牛同期发情处理的效果分析

为了对不同繁殖体况的母牦牛采取不同程度的补饲方案,研究补饲对母牦牛同期发情处理后发情和受胎的影响,从而探讨环湖地区牦牛繁殖率低下的形成机理及解决方案。试验选用青海省环湖地区3～10岁营养体况相近的受体母牦牛352头,按试验要求随机将青年牛、经产牛分为3组,分别补饲0,0.5,1.0 kg饲料,补饲时间为3个月,补饲结束后实施同期发情试验和定时授精,授精后40～60 d采用直肠检查法检查受胎情况。结果表明:补饲前与补饲后相比青年牛、经产牛营养状况差异显著(P<0.05),不同卵巢机能状态对母牦牛的发情率和受胎率的影响差异极显著(P<0.01),补饲Ⅱ组和补饲Ⅰ组青年牛、经产牛的发情率和受胎率与对照组相比差异极显著(P<0.01),补饲Ⅱ组与补饲Ⅰ组青年牛、经产牛同期发情处理后的发情率和受胎率与对照组差异显著(P<0.05)。说明母牦牛体内营养储备状况是制约牦牛繁殖率的关键因素,在传统的放牧条件下通过适度补饲能够改善母牦牛的繁殖体况和卵巢机能状态,可明显提高母牦牛的发情率和受胎率。     

牛SRY基因的克隆与测序

以牛基因组DNA为模板,在一对特异性引物的引导下,利用PCR技术成功地扩增了牛SRY基因。纯化回收目的片段,克隆到pMD 18-T载体上,筛选阳性克隆测序,得到SRY基因两端的序列(5′端641 bp,3′端786bp)。与网上公布的牛SRY基因序列进行同源性比较,结果显示,牛SRY基因长度为2 713 bp,克隆的5′和3′端2个片段与网上公布的牛SRY基因序列对应片断的同源性分别为98.7%和98.1%。     

山羊-绵羊种间体细胞核移植重构胚构建

以山羊皮肤成纤维细胞为供体细胞,绵羊去核卵母细胞为受体细胞进行种间体细胞核移植,构建山羊-绵羊重构胚,并对其采用离子霉素联合6-DM AP法和胞质注射绵羊精子提取物法进行激活,比较两种不同激活方法对山羊-绵羊种间体细胞核移植重构胚的激活效果。结果表明,共构建获得山羊-绵羊重构胚238枚,重构胚在体外能够发育至囊胚阶段;离子霉素联合6-DM AP法激活的卵裂率为58.33%(70/120),囊胚发育率为4.28%(3/70);胞质注射绵羊精子提取物法激活的卵裂率为63.55%(75/118),囊胚发育率为6.66%(5/75),两种方法的激活效果差异不显著。     

精子提取液对绵羊同异种核移植重构胚的激活效果

应用绵羊精子提取物体外激活绵羊体细胞核移植胚和山羊-绵羊异种核移植胚,比较了不同浓度的绵羊精子提取物对绵羊同种和异种重构胚的激活作用,并与传统的化学激活方法对重构胚的卵裂率、囊胚率和囊胚细胞数做了比较。结果表明,绵羊精子提取物可以有效地激活绵羊体细胞核移植胚和山羊-绵羊异种核移植胚,每一重构胚注射3pL浓度为5mg／mL的绵羊精子提取物,对同种和异种胚的发育效果均为最好,并显著高于其他试验组（P〈0．05）。注射绵羊精子提取物对绵羊-山羊异种核移植胚的卵裂率、囊胚率和囊胚细胞数的影响,与离子霉素联合6-DAMP激活法差异不显著（P〉0．05）。注射绵羊精子提取物对绵羊同种核移植胚的卵裂率和囊胚率的影响,与离子霉素联合6-DAMP激活法差异也不显著（P〉0．05）,但精子提取物注射法的绵羊核移植胚的囊胚细胞数显著（P〈0．05）高于化学激活法的细胞数（72．4vs65．2）。