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GJ EAMENS PD KIRKLAND MJ TURNER JA GARDNER† MP WHITE‡ CL HORNITZKY 《Australian veterinary journal》1988,65(4):120-123
Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida. 相似文献
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Dennis McIntosh Erin Ryder Gary Dickenson Kevin Fitzsimmons 《Journal of the World Aquaculture Society》2004,35(4):506-512
In order to reduce the impact that trout farm effluent can have on receiving water, it imperative to reduce the overall phosphorus (P) loading of these effluents. In response, the United States Environmental Protection Agency (US EPA) is issuing new effluent regulations for aquaculture. Therefore, efficient removal of P from effluent water is becoming increasingly important. The goal of this project was to determine the leaching rate of total and reactive P from trout feces under different water temperatures and water velocities immediately after evacuation. Scatter plots of the P load in the experimental containers over time were created for each fish/fecal sample and regression analysis was used to determine P leaching rates. Results from this study suggest that reactive phosphorus leaching rates are not affected by water velocities between 0.027 and 0.134 m/sec. However, at higher temperatures, reactive P leaches 1.92-mg PO4 /hr per g feces faster ( F 1.52 = 4.6445, P = 0.0358) than at lower temperatures. Mean reactive phosphorus leaching rates were 2.88 ± 0.704 and 0.96 ± 0.581mg PO4 /hr/g feces, for the high and low temperatures, respectively. On the other hand, total P does not appear to be affected by either water velocity or temperature. Mean total P leaching rate is 4.50 ± 1.053 mg PO4 /hr per g feces. This study suggests that removal time of feces from the system is critical in reducing the overall P load in effluent. 相似文献
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Evaluation of an Immunochromatographic Test to the Diagnosis of Canine Brucellosis Caused by Brucella canis 下载免费PDF全文
LB Keid JA Diniz TMFS Oliveira HL Ferreira RM Soares 《Reproduction in domestic animals》2015,50(6):939-944
This study evaluated the performance of an immunochromatographic test (ICT) for the diagnosis of canine brucellosis caused by Brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2‐mercaptoethanol and the agar gel immunodiffusion test (AGID). The microbiological culture, PCR and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and PCR in blood, semen and vaginal swab, a total of 102 dogs were divided into three groups: B. canis‐infected dogs (Group 1), B. canis‐non‐infected dogs (Group 2) and dogs with suspected brucellosis (Group 3). The diagnostic sensitivity of RSAT, 2ME‐RSAT, AGID and ICT in Group 1 was, respectively, 75%, 37.5%, 27.8% and 89.58%. The diagnostic specificity of RSAT, 2ME‐RSAT, AGID and ICT in Group 2 was, respectively, 91%, 100%, 100%, and 100%. In dogs with suspected brucellosis, 9.67% were RSAT positive, none was positive by 2ME‐RSAT, 3.22% were AGID positive and 6.45% were ICT positive. The main drawback concerning canine brucellosis diagnosis is the lack of a highly sensitive serological assay to be used as a screening test to the rapid identification of infected animals. The ICT showed a high diagnostic specificity and a diagnostic sensitivity value greater than that observed in the RSAT, 2ME‐RSAT and AGID. However, 10.41% of infected dogs had negative results by ICT. These dogs were positive by microbiological culture and/or PCR, indicating active infection and consequently a higher potential of spreading Brucella. Although rapid and simple to perform, the ICT lacked sensitivity to be used as a screening test. 相似文献