Continental drainage in north america during the phanerozoic from Nd isotopes

Neodymium (Nd) isotopic data show consistent patterns in the sources of sedimentary rocks in North America at a continental scale. Between 600 and 450 million years ago (Ma), ancient continental shield sources dominated. Around 450 Ma, detritus from the Caledonian-Appalachian mountains overwhelmed sediment from all older sources, and is documented over large areas of the southern, western, and northern margins of North America. This material continued to dominate the sediment supply until about 150 Ma, probably due to cannibalistic recycling of sedimentary rocks formed earlier. Around 150 Ma, the rising western Cordillera delivered new and different detritus to the sedimentary system.     

Phylogenetic analyses and molecular epidemiology of European salmonid alphaviruses (SAV) based on partial E2 and nsP3 gene nucleotide sequences

Sequence data were generated for portions of the E2 and nsP3 genes of 48 salmonid alphaviruses from farmed Atlantic salmon (AS), Salmo salar L., and rainbow trout (RT), Oncorhynchus mykiss (Walbaum), in marine and freshwater environments, respectively, from the Republic of Ireland, Northern Ireland, England, Scotland, Norway, France, Italy and Spain between 1991 and 2007. Based on these sequences, and those of six previously published reference strains, phylogenetic trees were constructed using the parsimony method. Trees generated with both gene segments were similar. Clades corresponding to the three previously recognized subtypes were generated and in addition, two further new clades of viruses were identified. A single further strain (F96-1045) was found to be distinct from all of the other strains in the study. The percentage of nucleotide divergence within clades was generally low (0-4.8% for E2, 0-6.6% for nsP3). Interclade divergence tended to be higher (3.4-19.7% for E2, 6.5-28.1% for nsP3). Based on these results and using current SAV terminology, the two new clades and F96-1045 were termed SAV subtypes 4, 5 and 6, respectively. SAV4 contained AS strains from Ireland and Scotland, while SAV5 contained only Scottish AS strains. Recently identified SAV strains from RT in Italy and Spain were shown to belong to SAV2. In addition, marine AS strains belonging to SAV2 were identified for the first time. Analysis of the origin of several clusters of strains with identical E2 and nsP3 sequences strongly support horizontal transmission of virus between farms and aquaculture companies. Evidence in support of vertical transmission was not found.     

Harvest,trade and conservation of the Asian arowana Scleropages formosus in Cambodia

• 1. The worldwide trade in aquarium fish is a multi‐billion dollar industry. One of the most popular and expensive species traded is the Asian arowana Scleropages formosus. As a result of over‐harvesting for the aquarium fish trade, S. formosus is now endangered and commercial international trade in the species is prohibited under CITES. Despite this, very little is known about S. formosus in the wild.
• 2. Interviews were conducted with 62 local villagers at four sites in Koh Kong province, south‐west Cambodia, to obtain information on the reproduction, historical and current harvest and trade, and population trends of S. formosus.
• 3. The spawning season of S. formosus in Cambodia begins towards the end of the dry season (March–April) and is approximately 3 months in duration. Each male mouth‐broods an average of 30 juveniles. Harvesters target S. formosus fry and juveniles, and typically kill or frighten brooding males into releasing their offspring in order to harvest them.
• 4. The prices obtained by harvesters for S. formosus has been increasing since collection began, and currently averages $US11–13 per juvenile. After collection, fish are transported through a series of larger towns, most ultimately bound for Thailand. The current number of S. formosus harvested from the wild in Cambodia is almost certainly highly unsustainable, with all respondents indicating that local S. formosus population abundance had declined dramatically in recent years. It is also likely that a number of populations have already become locally extinct.
• 5. Strengthening legislation to protect the species within Cambodia, increasing enforcement of cross‐border trade, and an investigation into alternative low‐impact income generation opportunities for local communities is vital. There is also an urgent need to gather detailed information on the distribution, population abundance and threats facing S. formosus in Cambodia, and in other countries throughout its range.

Copyright © 2008 John Wiley & Sons, Ltd.     

Measurement of Human Heart Rate during Usual Activty

A small, rugged, self-contained cumulative heart-beat counter has a transistorized amplifier, a watch movement made into a counter, and a battery. The counter is activated by each R-wave of the electrocardiographic complex obtained from newly designed precordial electrodes. Heart beats can be counted for as long as 24 hours.     

Systemic Immunosuppression by Methylprednisolone and Pregnancy Rates in Goats Undergoing the Transfer of Cloned Embryos

The presence of the zona pellucida has been perceived as a requirement for the oviductal transfer of cloned embryos at early stages of development while protecting the embryo from an immune system response. We hypothesized that steroid hormone therapy could reduce a potential cellular immune response after the transfer of zona‐free cloned embryos into the oviduct of recipient female goats. In Experiment 1, seven does were used to study the systemic immunosuppressant effect of the methylprednisolone administration (for 3 days) on blood cell counts. Whole blood was collected prior to treatment with methyprednisolone and then on Days 1, 2, 3, 4, 7, 14, 21 and 28 after the first dose of methylprednisolone for the analysis of haematological parameters. Methylprednisolone treatment significantly reduced circulating white blood cells and neutrophils in comparison with pre‐treatment levels, demonstrating a systemic immunosuppressant effect. In Experiment 2, a group of 58 does were used as recipient females to study the effect of administration of methylprednisolone for 3 days on the establishment of pregnancies after the transfer of zona‐free cloned embryos into the oviducts. No effects on pregnancy rates on Day 30 were observed regarding the distinct treatment groups (control vs. methylprednisolone), the source of oocytes (in vivo‐ vs in vitro‐matured) or the presence or absence of the zona pellucida in embryos. In summary, methylprednisolone was effective at inducing a systemic immunosuppressed state in goats, but the treatment prior to embryo transfer did not affect pregnancy rates. Moreover, pregnancy rates were similar between zona‐free and zona‐intact goat cloned embryos.     

Sexual Precocity of Nellore Bulls that are Offspring of Super Precocious,Precocious and Normal Cows in Extensive Farming Conditions

The objective of this work was to verify that mothers classified as super precocious (M1) and precocious (M2) produce more precocious bulls than females classified as normal (M3). This study included 21 186 animals with an average age of 21.29 ± 1.77 months that underwent a breeding soundness evaluation from 1999 to 2008. Of these animals, 2019, 6059 and 13 108 were offspring of M1, M2 and M3 females, respectively. In the breeding soundness examination, the animals were classified as sound for reproduction, sound under a natural mating regime, unsound for reproduction and discarded. To compare the averages obtained for each category of mother within the individual breeding soundness classes, a chi‐square test with a 5% error probability was used, considering the effects of year and month of birth and farm. For the three classes of mothers (M1, M2 and M3), 67.26, 67.22 and 64.16% of bull calves were considered sound for reproduction and 19.71, 19.46 and 21.90% were considered unsound for reproduction, respectively. There was no difference in the frequency of animals that were sound for reproduction under the natural breeding regime between the three classes of mothers (8.87, 9.31 and 9.19%, respectively). There was a difference between the numbers of precocious and normal females that were discarded, with frequencies of 4.01 and 4.75%, respectively (p < 0.05). There were differences in year and month of birth and farm between super precocious and precocious cows in relation to the breeding soundness classification of the animals. It was concluded that the bull offspring of super precocious and precocious cows presented a higher percentage of approval in the breeding soundness examination than the bull offspring of normal cows, demonstrating that the selection for precocity of females has contributed to an increase in the sexual precocity of the herd in relation to the sexual maturity of bulls.