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71.
AIM: To investigate the affected proteins by tumor necrosis factor (TNF)-α in endothelial cells, and further explore the potential molecular mechanism of TNF-α on endothelial cells. METHODS: Nitric oxide (NO) production in the cultured human umbilical vein endothelial cells (HUVECs) was measured by a NO assay kit. Proteomic alterations were analyzed using two-dimensional electrophoresis, and peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. RESULTS: NO production in HUVECs decreased significantly after TNF-α treatement. Proteomics analysis showed 21 protein spots were changed including 9 spots that were increased and 11 spots that were decreased after TNF-α stimulation, and 1 spot was only detected in TNF-α activated cell gels. CONCLUSIONS: Decreased the expression of ecNOS by TNF-α might result in decreased NO production. Up-regulated MAP/ERK kinase 3 expression might imply that TNF-α activates the expression of adhesion molecules. Cytoskeletal protein actin is also involved in TNF-α injuried HUVECs. Proteomic analysis can find some clues for identifying new potential target of TNF-α. 相似文献
72.
AIM: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. METHODS: The contraction of venous ring was measured by means of perfusion in vitro. The intimal proliferation and proliferation of cultured smooth muscle cells (vascular smooth muscle cells, VSMCs) were observed by the means of computerised image analysis and MTT method, respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. RESULTS: 1 week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they were more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control). TEA (blocker of Ca2+ activated potassium channels) increased MTT A490 value of VSMCs from femoral vein in a dose-dependent manner (P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts 1-4 weeks after transplantation (P<0.05, n=5). CONCLUSION: KCa was inhibited in autogenous vein graft, which accounted for vasospasm and intimal proliferation. 相似文献
73.
AIM: To investigate the influence of irbesartan (Irb), a new angiotensin II receptor 1 antagonist, on renal hypertrophy in streptozotocin (STZ)-induced diabetic rats. METHODS: Sprague-Dawley (SD) rats were randomly divided into three groups: normal group (Group N, n=7), diabetic group (Group DN, n=6) and irbesartan treated group (Group DNI, n=7). In the experimental group, after the rats subjected to uninephrectomy, STZ was given by peritoneally injection at bolus dose of 50 mg/kg to induce diabetes. Blood glucose (BG), body weight (BW), urinary albumin excretion (Ualb), 24 hour proteinuria (24 h Upro) were measured at week 4, 8, 12, respectively. By the end of experiment at week 12, creatinine clearance (Ccr), kidney weight (KW), indicator of renal hypertrophy (KW/BW), renal total protein content (RTP), glomerular area (AG) and glomerular volume (VG) as well as glomerular basement membrane (GBM) were determined by semi-quantitative pathology technique. RESULTS: It was showed that there was no significant difference in BG between group DN and DNI, while Irb significantly reduced the increasing of Ualb, 24 h Upro in diabetic rats compared to control group (P<0.01, respectively). Furthermore, Irb markedly inhibited the increasing of KW, KW/BW, RTP, AG, VG in diabetic rats (P<0.05, P<0.01, respectively). Of interest, Irb significantly prevented the increasing of GBM in diabetic rats. CONCLUSION: Irb exerts its early renal protective action by reducing proteinuria and inhibiting renal hypertrophy as well as the thickening of GBM. 相似文献
74.
对披碱草和野大麦及其杂种F1与BC1F1代的生物学及农艺特性进行了分析比较。结果表明,亲本野大麦生长发育节律较快,较早地开花结实,果后营养期较长,生育期74d,生长天数达206d,具有很强的分蘖能力;披碱草生长发育节律较慢,生育期124d,生长天数193d;杂种F1的生长天数介于双亲之间,生长动态偏向亲本披碱草,分蘖能力介于双亲之间;BC1F1代生长发育节律较F1代提早,不同株系生长天数不同,生长动态偏向轮回亲本野大麦,分蘖能力有较大的变异。杂种F1代表现出较强的杂种优势,BC1F1代产草量较F1代有所降低,不同株系间存在明显的差异。总体上,BC1F1代的生产性能倾向于轮回亲本野大麦。 相似文献
75.
M. C. Owen BVSC C. R. Lamb MA VETMB D. Lu BVET. MED. MVM M. P. Targett BA VETMB PHD 《Veterinary radiology & ultrasound》2004,45(2):149-155
The aim of this study was to determine the prevalence and potential significance of finding material in the middle ear of dogs having magnetic resonance (MR) imaging. Of 466 MR studies reviewed, an increased signal was identified in the tympanic bulla in 32 (7%) dogs. Cavalier King Charles spaniels, Cocker spaniels, Bulldogs, and Boxers were over-represented compared to the population of dogs having MR imaging. Five (16%) dogs had definite otitis media and one (3%) had a meningioma invading the middle ear. Of the remaining dogs, 13 (41%) had possible otitis media and 13 (41%) had neurologic conditions apparently unrelated to otitis media. The most common appearance of material in the middle ear was isointense in T1-weighted images and hyperintense in T2-weighted images. There was no apparent correlation between the signal characteristics of the material and the diagnosis. Enhanced signal after gadolinium administration was observed affecting the lining of the bulla in dogs with otitis media and in dogs with unrelated neurologic conditions. In dogs without clinical signs of otitis media, finding an increased signal in the middle ear during MR imaging may reflect subclinical otitis media or fluid accumulation unrelated to inflammation. Brachycephalic dogs may be predisposed to this condition. 相似文献
76.
Adherence of Actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin
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Roberto Hamer-Barrera Delfino Godínez V. Idalia Enríquez Sergio Vaca-Pacheco Rodrigo Martínez-Zú?iga Patricia Talamás-Rohana Francisco Suárez-Güemez Mireya de la Garza 《Canadian journal of veterinary research》2004,68(1):33-41
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetraacetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract. 相似文献
77.
I. Jiménez-Hidalgo G. Virgen-Calleros O. Martínez-de la Vega G. Vandemark V. Olalde-Portugal 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):317-331
Agave tequilana is the raw material for the production of the alcoholic beverage tequila. A bacterial disease has affected the A. tequilana crop in recent years. Previous reports based on colony and cell morphology, Gram stain and potato rot indicated that Erwinia sp. is the main pathogen. We isolated a several bacterial isolates capable of producing soft-rot symptoms in greenhouse pathogenicity assays. An extensive characterisation involving pathogenicity tests, fatty acid profile, metabolic and physiological properties, ribosomal DNA sequence and intergenic transcribed spacer amplification (ITS-PCR) and restriction banding pattern (ITS-RFLP) was made of each isolate. Three different species: Erwinia cacticida, Pantoea agglomerans and Pseudomonas sp. were identified. Fatty acid and metabolic profiles gave low similarity values of identification but 16S rDNA sequence, ITS-PCR and ITS-RFLP confirmed the identification of E. cacticida. In the phylogenetic tree, E. cacticida from blue agave was grouped neither with E. cacticida type strains nor with Erwinia carotovora. This is the first report that associates E. cacticida with A. tequilana soft-rot symptoms. 相似文献
78.
F.J. López-Escudero C. del Río J.M. Caballero M.A. Blanco-López 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):79-85
Resistance of 23 important olive cultivars to Verticillium dahliae has been evaluated in four experiments under controlled conditions. Nine-month-old nursery olive plants were inoculated with a cotton non-defoliating (ND) (V4) or a cotton defoliating (D) (V117) isolate of V. dahliae. Resistance was evaluated by assessing symptom severity using a 0–4 rating scale and estimating the area under disease progress curves. The percentage of plants killed and of those which recovered from the disease were used as additional parameters for including a particular cultivar into a defined category. Most of the evaluated cultivars were susceptible, although at different levels, to both isolates of V. dahliae. All cultivars were more susceptible to the D pathotype than to the ND one. A group of 11 cultivars, including several important Spanish cultivars, were susceptible or extremely susceptible to both pathotypes of V. dahliae. A second group showed differences of resistance depending on the pathotype used. They were susceptible or extremely susceptible to the D pathotype but resistant or moderately susceptible to the ND one. Finally, 'Frantoio', 'Oblonga' and 'Empeltre' were moderately susceptible to the D isolate of V. dahliae and resistant to the ND one. The resistance of 'Empeltre' was evident by the plant ability to recover from infection with either isolates. 'Empeltre' is considered to be a valuable cultivar for inclusion in breeding programmes for resistance to Verticillium wilt. 相似文献
79.
草地螟和黄草地螟危害苜蓿产量损失及防治指标的研究 总被引:2,自引:0,他引:2
采用人工接虫的方法,研究了草地螟的虫口密度与苜蓿产量损失之间的关系和防治指标.结果表明,苜蓿干草产量损失率(y)与20枝条虫量(x)的关系为y=4.936 6x-0.850 9,相关系数r为0.994 3,二者相关性极显著.按照防治的直接收益与直接成本,确定了宁夏旱地苜蓿和水地苜蓿草地螟的防治指标,旱地苜蓿刈割2和3次的草地螟的防治指标分别是10和14头/100枝,水地苜蓿草地螟的防治指标是7头/100枝.依据同样的试验方法,制定了黄草地螟危害苜蓿的防治指标,旱地苜蓿刈割2和3次的黄草地螟的防治指标分别是45和60头/100枝,水地苜蓿黄草地螟的防治指标是36头/100枝. 相似文献
80.
中草药饲料添加剂提高奶牛产奶量效果研究 总被引:14,自引:2,他引:14
选取同一胎次,健康正常的黑白花奶牛共36头随机分三组,对照组饲喂基础日粮,中草药Ⅰ组饲喂含5%黄芪日粮,中草药Ⅱ组饲喂党参-黄芪复合中药添加剂日粮,进行产奶量比对分析试验.结果证明,在奶牛的日粮中适当添加一定量的中草药添加剂 ,可以明显提高奶牛的奶产量(P<0.01),产奶量可提高约12.88%左右,并能有效减少奶牛乳房炎的发病率.试验结果还证明,在日粮中添加单味中药添加剂,对奶产量的提高也有一定的影响,但实际效果不如中草药Ⅱ组显著. 相似文献