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91.
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Transducin activation by rhodopsin without a covalent bond to the 11-cis-retinal chromophore 总被引:3,自引:0,他引:3
Rhodopsin and the visual pigments are a distinct group within the family of G-protein-linked receptors in that they have a covalently bound ligand, the 11-cis-retinal chromophore, whereas all of the other receptors bind their agonists through noncovalent interactions. The retinal chromophore in rhodopsin is bound by means of a protonated Schiff base linkage to the epsilon-amino group of Lys-296. Two rhodopsin mutants have been constructed, K296G and K296A, in which the covalent linkage to the chromophore is removed. Both mutants form a pigment with an absorption spectrum close to that of the wild type when reconstituted with the Schiff base of an n-alkylamine and 11-cis-retinal. In addition, the pigment formed from K296G and the n-propylamine Schiff base of 11-cis-retinal was found to activate transducin in a light-dependent manner, with 30 to 40% of the specific activity measured for the wild-type protein. It appears that the covalent bond is not essential for binding of the chromophore or for catalytic activation of transducin. 相似文献
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Acquisition by innervated cardiac myocytes of a pertussis toxin-specific regulatory protein linked to the alpha 1-receptor 总被引:4,自引:0,他引:4
S F Steinberg E D Drugge J P Bilezikian R B Robinson 《Science (New York, N.Y.)》1985,230(4722):186-188
During development, the chronotropic response of rat ventricular myocardium to alpha 1-adrenergic stimulation changes from positive to negative. The alpha 1-agonist phenylephrine increases the rate of contraction of neonatal rat myocytes cultured alone but decreases the rate of contraction when the myocytes are cultured with functional sympathetic neurons. The developmental induction of the inhibitory myocardial response to alpha 1-adrenergic stimulation in intact ventricle and in cultured myocytes was shown to coincide with the functional acquisition of a substrate for pertussis toxin. A 41-kilodalton protein from myocytes cultured with sympathetic neurons and from adult rat myocardium showed, respectively, 2.2- and 16-fold increases in pertussis toxin-associated ADP-ribosylation (ADP, adenosine diphosphate) as compared to controls. In nerve-muscle cultures, inhibition of the actions of this protein by pertussis toxin-specific ADP-ribosylation reversed the mature inhibitory alpha 1-adrenergic response to an immature stimulatory pattern. The results suggest that innervation is associated with the appearance of a functional pertussis toxin substrate by which the alpha 1-adrenergic response becomes linked to a decrease in automaticity. 相似文献
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Sex differences in verbal and performance IQ's of children undergoing open-heart surgery 总被引:1,自引:0,他引:1
Boys with congenital heart defects had essentially normal Verbal and Performnance IQ's on preoperative and postoperative tests; but girls' Verbal IQ's were significantly lower than those of boys, and significantly lower than girls' own Performance IQ's. This sex difference among congenital heart cases reverses the usual finding that girls excel on Verbal tests. 相似文献
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Shi Y Zhang C Zhang H Bechtel JH Dalton LR Robinson BH Steier WH 《Science (New York, N.Y.)》2000,288(5463):119-122
Electro-optic (EO) modulators encode electrical signals onto fiber optic transmissions. High drive voltages limit gain and noise levels. Typical polymeric and lithium niobate modulators operate with halfwave voltages of 5 volts. Sterically modified organic chromophores have been used to reduce the attenuation of electric field poling-induced electro-optic activity caused by strong intermolecular electrostatic interactions. Such modified chromophores, incorporated into polymer hosts, were used to fabricate EO modulators with halfwave voltages of 0.8 volts (at a telecommunications wavelength of 1318 nanometers) and to achieve a halfwave voltage-interaction length product of 2.2 volt-centimeters. Optical push-pull poling and driving were also used to reduce halfwave voltage. This study, together with recent demonstrations of exceptional bandwidths (more than 110 gigahertz) and ease of integration (with very large scale integration semiconductor circuitry and ultra-low-loss passive optical circuitry) demonstrates the potential of polymeric materials for next generation telecommunications, information processing, and radio frequency distribution. 相似文献
100.
The instantaneous California Current is seen to consist of intense meandering current filaments (jets) intermingled with synoptic-mesoscale eddies. These quasi-geostrophic jets entrain cold, upwelled coastal waters and rapidly advect them far offshore; this behavior accounts for the elongated, cool surface features that are seen extending across the California Current region in satellite infrared imagery. The associated advective mechanism should provide significant cross-shore transports of heat, nutrients, biota, and pollutants. The dynamics of the current system should be crucially influenced by its highly variable structure. 相似文献