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Fossil zooplankton fecal pellets found in thinly bedded marine and lacustrine black shales associated with phosphate, oil, and coal deposits, link the deposition of organic matter and biologically associated minerals with planktonic ecosystems. The black shales were probably formed in the anoxic basins of coastal marine waters, inland seas, and rift valley lakes where high productivity was supported by runoff, upwelling, and outwelling. 相似文献
75.
Evidence that the v-sis gene product transforms by interaction with the receptor for platelet-derived growth factor 总被引:14,自引:0,他引:14
A scheme for partial purification of biologically active v-sis-coded protein from cells transformed with simian sarcoma virus (SSV) has made possible a functional comparison of the transforming protein with platelet-derived growth factor (PDGF). The SSV-transforming gene product is capable of specifically binding PDGF receptors, stimulating tyrosine phosphorylation of PDGF receptors, and inducing DNA synthesis in quiescent fibroblasts. Each of these activities was specifically inhibited by antibodies to different regions of the v-sis gene product. Moreover, viral infection of a variety of cell types revealed a strict correlation between those cells possessing PDGF receptors and those susceptible to transformation by SSV. These findings provide evidence that SSV-transforming activity is mediated by the interaction of a virus-coded mitogen with PDGF receptors. 相似文献
76.
Sphingid moths (Manduca sexta) were reared for several generations on an artificial diet deficient in vitamin A and its precursors. Retinal tissue from depleted moths was removed for histological examination. There was extensive histolysis in the retinal epithelium and underlying nervous and connective tissues. This pathology correlated with severe visual impairment, even though normal growth, metamorphosis, and reproduction occurred. In the adult this pathology could be reversed when the larvae were reared only on tobacco (its usual host) or on the artificial diet supplemented with beta-carotene or vitamin A palmitate. 相似文献
77.
The role of individual cysteine residues in the structure and function of the v-sis gene product 总被引:12,自引:0,他引:12
The v-sis oncogene encodes a platelet-derived growth factor (PDGF)-related product whose transforming activity is mediated by its functional interaction with the PDGF receptor. PDGF, as well as processed forms of the v-sis gene product, is a disulfide-linked dimer with eight conserved cysteine residues in the minimum region necessary for biologic activity. Site-directed mutagenesis of the v-sis gene revealed that each conserved cysteine residue was required directly or indirectly for disulfide-linked dimer formation. However, substitution of serine for cysteine codons at any of four positions had no detrimental effect on transforming activity of the encoded v-sis protein. These results establish that interchain disulfide bonds are not essential in order for this protein to act as a functional ligand for the PDGF receptor. The remaining four substitutions of serine for cysteine each inactivated transforming function of the molecule. In each case this was associated with loss of a conformation shown to involve intramolecular disulfide bonds. These studies provide insight into the role of individual cysteine residues in determining the structure of the sis/PDGF molecule critical for biological activity. 相似文献
78.
PWM Kronen DVM PF Moon-Massat DVM DipACVA JW Ludders DVM DipACVA RD Gleed BVSc MRCVS DVA DipACVA DipECVA TJ Kern DVM DipACVO J Randolph DVM DipACVIM H N Erb DVM MS PhD 《Veterinary anaesthesia and analgesia》2001,28(3):146-155
Objective To evaluate the effectiveness of two insulin doses to maintain an acceptable range of blood glucose concentrations (70–200 mg dL?1) in the peri‐operative period in diabetic dogs. Animals Twenty‐four diabetic dogs with a median weight of 20.6 kg and a median age of 8 years old. Methods The dogs were randomly assigned to receive either 25 or 100% of their normal insulin dose subcutaneously on the morning of surgery. The anesthetic and feeding protocols were standardized. On the day before surgery, venous blood was collected for measurement of β‐hydroxybutyrate, cholesterol, glucose, glycosylated hemoglobin, hematocrit, total plasma protein and urea nitrogen. On the day of surgery, blood glucose concentrations were measured prior to anesthesia, prior to the start of surgery, 1 and 2 hours after beginning of surgery, 1 hour after extubation, at 16 : 00 hours and at 20 : 00 hours. β‐hydroxybutyrate concentrations were measured at 20 : 00 hours that day. At 08 : 00 hours the following day, β‐hydroxybutyrate and glucose concentrations were measured. The significance of differences between groups was tested with Wilcoxon's two‐tailed rank‐sum test, Chi‐square test and Fisher's exact test. Results There were no differences in insulin treatments, clinical signs, concurrent diseases and most clinicopathological parameters between the two groups of dogs at entry to the study. The 25% dose group had blood glucose values of 296 (102–601) mg dL?1 at 16 : 00 hours and 429 (97–595) mg dL?1 at 20 : 00 hours on the day of surgery. The 100% insulin dose group had lower corresponding values of 130 (55–375) mg dL?1 (p = 0.04) and 185 (51–440) mg dL?1 (p = 0.004). No other differences (p < 0.05) were detected between the two groups. Conclusions The administration of a full dose of insulin is only marginally advantageous for reducing glucose to normal (70–120 mg dL?1) after anesthesia but neither dose consistently induced glycemic values in an acceptable range (70–200 mg dL?1) or normoketonemia. Clinical relevance Blood glucose should be measured immediately before anesthesia and periodically throughout the peri‐operative period in all diabetic dogs because presurgical subcutaneous administration of 25 or 100% of the normal insulin dose resulted in unpredictable blood glucose concentrations. 相似文献
79.
Kelm MA Johnson JC Robbins RJ Hammerstone JF Schmitz HH 《Journal of agricultural and food chemistry》2006,54(5):1571-1576
A new chromatographic approach for separating cacao procyanidins according to their degree of polymerization has been developed. It utilizes diol stationary phase columns operating in normal phase mode with a binary gradient of acidified acetonitrile and methanol-water. Performance of the diol stationary phase was evaluated on an analytical scale utilizing classical chromatographic conditions for the normal phase separation of procyanidins according to their degree of polymerization. The new separation approach was developed on an analytical scale but further extended to the preparative scale. These newly developed analytical and preparative high-performance liquid chromatography procedures were successfully applied to the separation, as well as isolation, of cacao procyanidins from unfermented cacao seeds. The degree of polymerization associated with each molecular weight fraction was determined by mass spectrometry. 相似文献
80.
Intraguild interactions and behavior of Spodoptera frugiperda and Helicoverpa spp. on maize 下载免费PDF全文