全文获取类型
收费全文 | 258篇 |
免费 | 19篇 |
专业分类
农学 | 1篇 |
基础科学 | 1篇 |
12篇 | |
综合类 | 60篇 |
农作物 | 8篇 |
水产渔业 | 3篇 |
畜牧兽医 | 180篇 |
园艺 | 3篇 |
植物保护 | 9篇 |
出版年
2021年 | 3篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 4篇 |
2015年 | 6篇 |
2014年 | 8篇 |
2013年 | 15篇 |
2012年 | 7篇 |
2010年 | 15篇 |
2009年 | 11篇 |
2008年 | 3篇 |
2007年 | 8篇 |
2006年 | 12篇 |
2005年 | 12篇 |
2004年 | 7篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 6篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 7篇 |
1992年 | 5篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1989年 | 9篇 |
1988年 | 2篇 |
1986年 | 3篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 4篇 |
1970年 | 6篇 |
1969年 | 9篇 |
1968年 | 4篇 |
1967年 | 3篇 |
1948年 | 2篇 |
1947年 | 4篇 |
1943年 | 2篇 |
1937年 | 2篇 |
1914年 | 1篇 |
1893年 | 1篇 |
排序方式: 共有277条查询结果,搜索用时 218 毫秒
271.
Human lupus inclusions and interferon 总被引:6,自引:0,他引:6
S A Rich 《Science (New York, N.Y.)》1981,213(4509):772-775
Raji cells, a human B lymphoblastoid cell line of Burkitt lymphoma origin, formed lupus inclusions when grown in a medium conditioned by the growth of Raji cells whose DNA thymidine residues had been unifilarly (single-strandedly) substituted with bromodeoxyuridine. Ultracentrifugation of this medium in excess of that required to remove Epstein-Barr virus and all other known mammalian viruses did not prevent the formation of the inclusions, and treatment of the conditioned medium with pronase destroyed the activity. These results demonstrate the presence of a protein that is secreted from bromodeoxyuridine-substituted Raji cells and is capable of inducing nonbromodeoxyuridine-substituted cells to form lupus inclusions. Interferon (100 units per milliliter) was found in the conditioned medium. Inclusions also formed in Raji cells grown in fresh medium supplemented with human leukocyte or fibroblast interferon (100 units per milliliter). 相似文献
272.
Cell-adhesive motif in region II of malarial circumsporozoite protein 总被引:19,自引:0,他引:19
The segment of the malarial circumsporozoite (CS) protein designated Region II is highly conserved among different malarial species. A similar sequence is also present in several other proteins, including thrombospondin, properdin, and a blood-stage antigen of Plasmodium falciparum. By means of peptides synthesized from sequences of the Plasmodium vivax CS protein in the vicinity of Region II, it was found that two overlapping 18- to 20-amino acid peptides promoted the adhesion of a variety of human hematopoietic cell lines. The amino acid sequence valine-threonine-cysteineglycine (VTCG), contained within this common motif, was shown to be the critical sequence for the observed cell-adhesive properties. 相似文献
273.
The molecular structure of a DNA-triostin A complex 总被引:31,自引:0,他引:31
A H Wang G Ughetto G J Quigley T Hakoshima G A van der Marel J H van Boom A Rich 《Science (New York, N.Y.)》1984,225(4667):1115-1121
The molecular structure of triostin A, a cyclic octadepsipeptide antibiotic, has been solved complexed to a DNA double helical fragment with the sequence CGTACG (C, cytosine; G, guanine; T, thymine; A, adenine). The two planar quinoxaline rings of triostin A bis intercalate on the minor groove of the DNA double helix surrounding the CG base pairs at either end. The alanine residues form hydrogen bonds to the guanines. Base stacking in the DNA is perturbed, and the major binding interaction involves a large number of van der Waals contacts between the peptides and the nucleic acid. The adenine residues in the center are in the syn conformation and are paired to thymine through Hoogsteen base pairing. 相似文献
274.
Bovine parathyroid tissue incubated in vitro secretes a protein that is distinct from both parathyroid hormone and proparathyroid hormone and comprises about 50 percent of the total secreted protein. This protein appears to be an aggregate consisting of two or more subunits of molecular weight 70,000 as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Although the function of this protein is unknown, the secretion rates of both the protein and parathyroid hormone respond in parallel to changes in the concentration of calcium in the medium. 相似文献
275.
Isotope-edited NMR of cyclosporin A bound to cyclophilin: evidence for a trans 9,10 amide bond 总被引:2,自引:0,他引:2
S W Fesik R T Gampe T F Holzman D A Egan R Edalji J R Luly R Simmer R Helfrich V Kishore D H Rich 《Science (New York, N.Y.)》1990,250(4986):1406-1409
The binding of a 13C-labeled cyclosporin A (CsA) analog to cyclophilin (peptidyl prolyl isomerase) was examined by means of isotope-edited nuclear magnetic resonance (NMR) techniques. A trans 9,10 peptide bond was adopted when CsA was bound to cyclophilin, in contrast to the cis 9,10 peptide bond found in the crystalline and solution conformations of CsA. Furthermore, nuclear Overhauser effects (NOEs) were observed between the zeta 3 and epsilon 3 protons of the methylleucine (MeLeu) residue at position 9 of CsA and tryptophan121 (Trp121) and phenylalanine (Phe) protons of cyclophilin, suggesting that the MeLeu9 residue of CsA interacts with cyclophilin. These results illustrate the power of isotope-edited NMR techniques for rapidly providing useful information about the conformations and active site environment of inhibitors bound to their target enzymes. 相似文献
276.
Venkatachalam M Teuber SS Peterson WR Roux KH Sathe SK 《Journal of agricultural and food chemistry》2006,54(4):1449-1458
Rabbit polyclonal antibody-based inhibition ELISA as well as immunoblotting analyses of proteins extracted from variously processed pecans (cv. Desirable) indicate that pecan proteins are antigenically stable. Pecan antigens were more sensitive to moist heat than dry heat processing treatments. SDS-PAGE and immunoblotting analysis of the native and heat-denatured proteins that were previously subjected to in vitro simulated gastric fluid digestions indicate that stable antigenic peptides were produced. Both enzyme-to-substrate ratio and digestion time were influential in determining the stability of pecan polypeptides. The stable antigenic polypeptides may serve as useful markers in developing assays suitable for the detection of trace amounts of pecans in foods. 相似文献
277.
Richer HB Anderson J Brewer J Davis S Fahlman GG Hansen BM Hurley J Kalirai JS King IR Reitzel D Rich RM Shara MM Stetson PB 《Science (New York, N.Y.)》2006,313(5789):936-940
NGC 6397 is the second closest globular star cluster to the Sun. Using 5 days of time on the Hubble Space Telescope, we have constructed an ultradeep color-magnitude diagram for this cluster. We see a clear truncation in each of its two major stellar sequences. Faint red main-sequence stars run out well above our observational limit and near to the theoretical prediction for the lowest mass stars capable of stable hydrogen burning in their cores. We also see a truncation in the number counts of faint blue stars, namely white dwarfs. This reflects the limit to which the bulk of the white dwarfs can cool over the lifetime of the cluster. There is also a turn toward bluer colors in the least luminous of these objects. This was predicted for the very coolest white dwarfs with hydrogen-rich atmospheres as the formation of H(2) and the resultant collision-induced absorption cause their atmospheres to become largely opaque to infrared radiation. 相似文献