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141.
When prototrophic yeast cells are cultured under nutrient-limited conditions that mimic growth in the wild, rather than in the high-glucose solutions used in most laboratory studies, they exhibit a robustly periodic metabolic cycle. Over a cycle of 4 to 5 hours, yeast cells rhythmically alternate between glycolysis and respiration. The cell division cycle is tightly constrained to the reductive phase of this yeast metabolic cycle, with DNA replication taking place only during the glycolytic phase. We show that cell cycle mutants impeded in metabolic cycle-directed restriction of cell division exhibit substantial increases in spontaneous mutation rate. In addition, disruption of the gene encoding a DNA checkpoint kinase that couples the cell division cycle to the circadian cycle abolishes synchrony of the metabolic and cell cycles. Thus, circadian, metabolic, and cell division cycles may be coordinated similarly as an evolutionarily conserved means of preserving genome integrity. 相似文献
142.
新化合物苦皮藤素Ⅱ,Ⅲ,Ⅳ的分离和结构鉴定简报 总被引:1,自引:0,他引:1
以叶碟定量载毒饲虫的方法追踪活性,经3次硅胶柱层析及反相高效液相色谱半制备,从杀虫植物苦皮藤(Celastrus angulatus,Max.)根皮的石油醚提取物中分离出3个对粘虫(Mythimnaseparata)幼虫具有强烈毒杀作用和麻醉作用的化合物B,D,T.利用高分辨质谱及核磁共振波谱鉴定了3个化合物的分子结构,结果说明,这些化合物都属于二氢沉香呋喃倍半萜酯类,其中B和T为一对异构体,不同之处在于C—9位的苯甲酰氧基的构型,B为χ构型,T为β构型。经文献检索,这3个化合物都是从未报道过的新化合物,分别命名为苦皮藤素Ⅱ,Ⅲ,Ⅳ。 相似文献
143.
推行农业标准化,是我国应对加入WTO、实施农业结构调整的一项基础性工作,直接关系到农业市场化、产业化、集约化、现代化的实现。应针对我国农业标准化发展的现状,积极借鉴发达国家的经验,健全和完善农业标准化五大体系。 相似文献
144.
油桐数量性状的主成分分析及分类 总被引:5,自引:2,他引:5
对湖南泸溪葡萄桐在鄂南栽培条件下的10个表型性状进行了主成分分析一分类。结果表明,前5个主成分的累计贡献率达80.9%。根据主成分的因子负荷量及其主要指标组合,将10个生物学性状分为3类,分别称之为冠形因子,花序因子,枝梢因子。主成分分析说明了这3类因子对油桐的作用。 相似文献
145.
146.
Kogovšek P Kladnik A Mlakar J Znidarič MT Dermastia M Ravnikar M Pompe-Novak M 《Phytopathology》2011,101(11):1292-1300
The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants of the highly susceptible cultivar Igor was investigated. Virus presence and accumulation was analyzed in different plant organs and tissues using real-time polymerase chain reaction and transmission electron microscopy (TEM) negative staining methods. To get a complete insight into the location of viral RNA within the tissue, in situ hybridization was developed and optimized for the detection of PVY RNA at the cellular level. PVY was shown to accumulate in all studied leaf and stem tissues, in shoot tips, roots, and tubers; however, the level of virus accumulation was specific for each organ or tissue. The highest amounts of viral RNA and viral particles were found in symptomatic leaves and stem. By observing cell ultrastructure with TEM, viral cytoplasmic inclusion bodies were localized in close vicinity to the epidermis and in trichomes. Our results show that viral RNA, viral particles, and cytoplasmic inclusion bodies colocalize within the same type of cells or in close vicinity. 相似文献
147.
水肥对高产无性系油茶果实产量的影响 总被引:1,自引:0,他引:1
以肥料氮(N)、磷(P)、钾(K)不同施用量和灌溉水量(H2O)为试验因子,采用二次通用旋转组合设计,研究了水肥对油茶鲜果产量的影响。结果表明,N、P、K和H2O的用量对油茶鲜果产量均存在显著的影响,且N与K、K与H2O对油茶鲜果产量存在显著的互作效应。试验因子间的交互作用使油茶鲜果产量明显提高,达到最大值时4个因子的编码值分别为:N为1.03、P为0.62、K为1.19、H2O为0.92,即全年每株油茶施有效养分N 181.8 g、P2O5 48.6 g、K2O 287.1 g,灌水29.2 kg,相当于每hm2施用N 795 kg、P2O5 80 kg、K2O 474 kg,灌水48 m3。采用该方案,油茶鲜果产量可达到最大为5 131 kg hm2。 相似文献
148.
Hoang Thi Thanh Thuy Nguyen Nhu Ha Vy Tu Thi Cam Loan 《Water, air, and soil pollution》2007,182(1-4):73-81
The aquatic system of Hochiminh City comprises two main rivers: the Sai Gon and Nha Be rivers. Five canals discharge into these two rivers: NhieuLoc-ThiNghe, TauHu-BenNghe, TanHoa-LoGom, ThamLuong-BenCat and Doi-Te. The rivers and these canals collect effluent water from domestic and industrial sources. Most of these flows are not treated or at most are only primarily treated. A total of 33 sediment cores were taken from these rivers and canals. Chemical composition of these aquatic sediments has very high concentrations of several “urban” metals such as Cd, Cr, Cu and Zn. Most of the samples have exceeded the US EPA’s toxicity reference values for Cu, Zn and Cr (82, 82 and 70%, respectively). The highest concentrations of these metals appear to be associated with the uncontrolled and untreated industrial runoff to the discharge canals. These concentrations in fluvial sediment are relatively low, which indicates the dilution process of the contaminants. This finding indicates that the anthropogenic inputs play an important role in the elevation of heavy metals in the aquatic system and organic matter seems to exert a strong geochemical control on the amount of heavy metals. The Pearson correlation coefficients calculated for Cd, Cr, Cu and Zn, are 0.89; 0.72; 0.93 and 0.87, respectively. 相似文献
149.
150.
卵孢白僵菌感染草地蛴螬的研究 总被引:3,自引:0,他引:3
从湖南南山牧场草地上自然罹病的蛴螬虫体上分离出一株卵孢白僵菌。在室内和野外人工草地进行蛴螬活虫感染试验,结果表明,在室内用卵孢白僵菌孢子液感染蛴螬活虫,30天内蛴螬感染率达64.6─92.1%;野外草地试验,用卵孢白僵菌制成菌剂拌少量细土撤于地表,20天内,蛴螬感染率为70%以上。 相似文献