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101.
102.
Kogovšek P Kladnik A Mlakar J Znidarič MT Dermastia M Ravnikar M Pompe-Novak M 《Phytopathology》2011,101(11):1292-1300
The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants of the highly susceptible cultivar Igor was investigated. Virus presence and accumulation was analyzed in different plant organs and tissues using real-time polymerase chain reaction and transmission electron microscopy (TEM) negative staining methods. To get a complete insight into the location of viral RNA within the tissue, in situ hybridization was developed and optimized for the detection of PVY RNA at the cellular level. PVY was shown to accumulate in all studied leaf and stem tissues, in shoot tips, roots, and tubers; however, the level of virus accumulation was specific for each organ or tissue. The highest amounts of viral RNA and viral particles were found in symptomatic leaves and stem. By observing cell ultrastructure with TEM, viral cytoplasmic inclusion bodies were localized in close vicinity to the epidermis and in trichomes. Our results show that viral RNA, viral particles, and cytoplasmic inclusion bodies colocalize within the same type of cells or in close vicinity. 相似文献
103.
水肥对高产无性系油茶果实产量的影响 总被引:1,自引:0,他引:1
以肥料氮(N)、磷(P)、钾(K)不同施用量和灌溉水量(H2O)为试验因子,采用二次通用旋转组合设计,研究了水肥对油茶鲜果产量的影响。结果表明,N、P、K和H2O的用量对油茶鲜果产量均存在显著的影响,且N与K、K与H2O对油茶鲜果产量存在显著的互作效应。试验因子间的交互作用使油茶鲜果产量明显提高,达到最大值时4个因子的编码值分别为:N为1.03、P为0.62、K为1.19、H2O为0.92,即全年每株油茶施有效养分N 181.8 g、P2O5 48.6 g、K2O 287.1 g,灌水29.2 kg,相当于每hm2施用N 795 kg、P2O5 80 kg、K2O 474 kg,灌水48 m3。采用该方案,油茶鲜果产量可达到最大为5 131 kg hm2。 相似文献
104.
Hoang Thi Thanh Thuy Nguyen Nhu Ha Vy Tu Thi Cam Loan 《Water, air, and soil pollution》2007,182(1-4):73-81
The aquatic system of Hochiminh City comprises two main rivers: the Sai Gon and Nha Be rivers. Five canals discharge into these two rivers: NhieuLoc-ThiNghe, TauHu-BenNghe, TanHoa-LoGom, ThamLuong-BenCat and Doi-Te. The rivers and these canals collect effluent water from domestic and industrial sources. Most of these flows are not treated or at most are only primarily treated. A total of 33 sediment cores were taken from these rivers and canals. Chemical composition of these aquatic sediments has very high concentrations of several “urban” metals such as Cd, Cr, Cu and Zn. Most of the samples have exceeded the US EPA’s toxicity reference values for Cu, Zn and Cr (82, 82 and 70%, respectively). The highest concentrations of these metals appear to be associated with the uncontrolled and untreated industrial runoff to the discharge canals. These concentrations in fluvial sediment are relatively low, which indicates the dilution process of the contaminants. This finding indicates that the anthropogenic inputs play an important role in the elevation of heavy metals in the aquatic system and organic matter seems to exert a strong geochemical control on the amount of heavy metals. The Pearson correlation coefficients calculated for Cd, Cr, Cu and Zn, are 0.89; 0.72; 0.93 and 0.87, respectively. 相似文献
105.
狂犬病病毒糖蛋白转基因小鼠的构建研究 总被引:1,自引:1,他引:0
将狂犬病病毒糖蛋白cDNA BglⅡ(1.67kb)片段正向插入pMT010/A^+BamHⅠ切点,构建重组质粒pMT010/A^+-Rgp,用EcoRⅠ+SalⅠ对pMT010/A^+-Rgp进行双酶切后,回收含有狂犬病病毒糖蛋白cDNA和绵羊MT启动子的2.76kb片段,通过显微注射技术将该片段注入小鼠单细胞受精卵雄前核内,在进行胚胎移植后,获得44只小鼠,经PCR、Southern杂交及原位 相似文献
106.
107.
卵孢白僵菌感染草地蛴螬的研究 总被引:3,自引:0,他引:3
从湖南南山牧场草地上自然罹病的蛴螬虫体上分离出一株卵孢白僵菌。在室内和野外人工草地进行蛴螬活虫感染试验,结果表明,在室内用卵孢白僵菌孢子液感染蛴螬活虫,30天内蛴螬感染率达64.6─92.1%;野外草地试验,用卵孢白僵菌制成菌剂拌少量细土撤于地表,20天内,蛴螬感染率为70%以上。 相似文献
108.
涂澄海 《东北农业大学学报》1993,(2)
建立了考虑空气阻力 R_B 的影响时质点在水平圆盘抛离后的运动微分方程并求得其解。可作为某些农业机械工作部件性能设计的理论基础。 相似文献
109.
油松毛虫优化管理决策的研究 总被引:1,自引:0,他引:1
在防治中,究竟采取那一种方案为最优方案,如何找出最优方案,并且应用这一最优方案可获取多大的效益,这是本文研究的目的。一、建模方法考虑到决策者的应用方便,我们在已求得动态经济阈值的基础上,通过最佳序贯决 相似文献
110.
Cheng IC Liang SM Tu WJ Chen CM Lai SY Cheng YC Lee F Huang TS Jong MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):859-864
Monoclonal antibodies (MAbs) reported here were produced against the porcinophilic foot-and-mouth disease virus (FMDV) that caused the devastating swine disease on 1997 in Taiwan. A panel (25) of MAbs were found to react with VP1 of O/Taiwan/97 (O/97) by ELISA with various potencies. The biological identities of these VP1 reacting MAbs, such as neutralization activity, isotype and capability to distinguish between two serotype O FMDVs, O/97 and O/Taiwan/KM1/99 (O/99), were further analyzed. Eleven out of the total eighteen O/97 neutralizing MAbs were able to neutralize heterologous O/99. Eight O/97 neutralizing and five non-neutralizing MAbs could differentiate two serotype O FMDVs by immunofluorescence assay (IFA) implied that these thirteen MAbs recognized O/97 specific epitope(s). Furthermore, reactivities of the VP1 reacting MAbs with a 29 amino acids synthetic peptide (P29) representing the betaG-betaH loop of VP1 were analyzed by ELISA and fourteen were found positive. MAb clone Q10E-3 reacting strongest with VP1 and P29, neutralizing both but not differentiating two serotype O viruses suggested that the antibody binding site might involve the RGD motif and its C terminal conserved region on betaG-betaH loop. MAbs with diverse characters presented in this study were the first raised against porcinophilic FMDV. The complete set of MAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV. 相似文献