Oxalate-degrading capacities of lactic acid bacteria in canine feces

In this study, lactic acid bacteria in canine feces were isolated and identified, and their oxalate-degrading capacities were evaluated. The oxalate-degrading capacities were determined for 24 of 47 (51.06%) lactic acid bacteria isolates. Of these, 8 isolates [Leuconostoc mesenteroides (RL75), Lactococcus garvieae (CD2), Lactococcus subsp. lactis (CS21), Enterococcus faecium (CL71 and CL72), and Enterococcus faecalis (CD14, CS62, and CD12)] degraded more than 5% of the oxalate present, while the others degraded less than 5% of the oxalate in vitro. Isolates that degraded more than 5% of the oxalate present were selected for further examination. The oxalate-degrading capacities of individual isolates, a mixture of Enterococcus, a mixture of Lactococcus, and a mixture of the eight isolates were evaluated in media containing different concentrations of glucose (sufficient, insufficient, or no glucose). In comparison with the control medium, all of the individual isolates and mixtures of isolates could degrade oxalate in all three groups (P<0.05). In most cases, the isolates growing in medium with 20 g/L of glucose had higher oxalate-degrading capacities than those growing in medium with 2.5 g/L of glucose or no glucose. The mixture of all isolates showed higher oxalate-degrading capacity than the individual isolates and other mixtures. The oxalate-degrading capacities of the isolates were isolate dependent.     

A pig–human comparative RH map comprising 20 genes on pig chromosome 13q41 that harbours the ETEC F4ac receptor locus

The enterotoxigenic Escherichia coli (ETEC) F4ac is a major cause of diarrhoea in newborn and young pigs. The locus for the intestinal ETEC F4ac receptor (F4acR) has been mapped to pig chromosome (SSC) 13q41 with known homology to human chromosome (HSA) 3q21 and q29. However, the causative gene and mutation(s) remain unknown. The aim of this study was to characterize gene-derived markers on SSC13q41 for fine mapping of the F4acR locus, and construct a high-resolution pig–human comparative map to select positional candidate genes for F4acR. Pig-specific sequence-tagged site markers were developed for 20 genes that are located in a 6.8-Mb region on HSA3q21 and q29, and a total of 34 single-nucleotide polymorphisms (SNPs) were identified in 14 of 20 markers developed. Eighteen markers were mapped to SSC13q41, while the other two markers ( PLXNA1 and KLF15 ) were assigned to SSC13q32 and SSC7q13, respectively, by radiation hybrid mapping. This result showed that there was a small conserved segment on SSC7 corresponding to HSA3q21. A framework map comprising 18 markers on SSC13q41 was established, refining the synteny breakpoint on SSC13q41 to a region of 12.3 centiRay. The comparative radiation hybrid (RH) map revealed three interesting candidate genes for F4acR from the human genome, viz. MUC4 , MUC13 and MUC20 . Linkage analysis with six marker polymorphisms revealed that MUC4 had the most significant linkage with the F4acR locus.     

饲用油腊的加工技术及应用

本文介绍了近几年在饲料工业生产中不同饲用油脂添加技术应用及研究,重点对常规喷涂技术、真空喷涂技术和固态油脂喷雾干燥技术进行综述、分析,并对饲料中应用的固态油脂生产加工和应用技术进行了展望。     

基于信息熵的天山山区降水时空变化特征

降水是山区气候中最重要的气象要素之一,其大小及变化直接关系到干旱山区中、下游的生态环境与经济社会的发展,但是,由于技术和地理条件的限制,是难以精确观测的气象要素。本研究采用天山山区31个台站和1960-2008年间的实测降水资料,应用信息熵方法对天山山区年、季和月的降水变化特征进行分析。结果表明,天山山区降水在时间和空间上均存在变异性,时间变异性表现为信息熵的无序指数(DI)年际变化小于季节变化,季节变化小于月变化。DI多年变化特征呈现下降趋势;冬季DI变化最为显著,夏季最小;冬季北坡降水随高程的增加DI变化呈显著增加趋势(P0.05),春、夏和秋季南坡降水随高程的增加DI变化呈显著减少趋势(P0.05)。山区月降水4-9月逐渐增加,10月至次年3月降水逐渐减少,月降水DI的变化与降水变化呈反相特征。在空间上DI变化与降水的分布相反,表现为南强北弱,东强西弱,伊犁河谷降水DI变化最小。结果证明,DI方法能够有效地分析山区降水的时空变化特征,为合理开发山区的水资源提供科学依据。     

Assessment of the effects of age and joint disease on hydroxyproline and glycosaminoglycan concentrations in synovial fluid from the metacarpophalangeal joint of horses

OBJECTIVE: To assess the effects of age and joint disease on hydroxyproline and glycosaminoglycan (GAG) concentrations in synovial fluid from the metacarpophalangeal joint of horses and evaluate the association of those concentrations with severity of osteoarthritis and general matrix metalloproteinase (MMP) activity. SAMPLE POPULATION: Synovial fluid was collected from the metacarpophalangeal joints of foals at birth (n = 10), 5-month-old foals (10), 11-month-old foals (5), and adult horses (73). PROCEDURE: Hydroxyproline and GAG concentrations were determined in synovial fluid samples. The severity of osteoarthritis in adult joints was quantified by use of a cartilage degeneration index (CDI) and assessment of general MMP-activity via a fluorogenic assay. RESULTS: Hydroxyproline and GAG concentrations in synovial fluid were highest in neonates and decreased with age. Concentrations reached a plateau in adults by 4 years and remained constant in healthy joints. In synovial fluid from osteoarthritic joints, hydroxyproline and GAG concentrations were not increased, compared with unaffected joints, but hydroxyproline were significantly correlated with the CDI and general MMP activity. There was no significant correlation between GAG concentration and CDI value or MMP activity. CONCLUSIONS AND CLINICAL RELEVANCE: Changes in hydroxyproline concentration in synovial fluid appeared to indicate damage to collagen of the articular cartilage. In joints with osteoarthritis, the lack of high GAG concentration in synovial fluid and the absence of a significant correlation between GAG concentration and CDI values or MMP activity may severely limit the usefulness of this marker for monitoring equine joint disease.     

Rapid identification and differentiation of Theileria sergenti and Theileria sinensis using a loop-mediated isothermal amplification (LAMP) assay

The present study developed and validated a species-specific loop-mediated isothermal amplification (LAMP) assay for the rapid detection and discrimination of two benign bovine Theileria species – T. sergenti and T. sinensis. The LAMP assay is inexpensive and easy to perform and involves a rapid reaction-the amplification can be performed in 55 min or 50 min under isothermal conditions of 61 °C or 63 °C, respectively, by employing a set of four species-specific primer mixtures. The results can be checked using agarose gels. The optimal assay conditions, under which the assay exhibited with no cross-reaction with other closely related tick-borne parasites (T. annulata, Babesia bovis, B. bigemina, B. major, B. ovata, B. U. sp., Anaplasma marginale) or between the two Theileria species of interest, was established. The assay is approximately 10-fold more sensitive than the conventional specific PCR assay. The LAMP assay was validated using DNA from 6 standard stocks in the laboratory and was evaluated for its diagnostic utility using blood samples collected from experimentally and naturally infection cattle or yaks in China. These findings indicate that this Theileria species-specific LAMP assay may have potential clinical applications for the detection and differentiation of two benign bovine Theileria species – T. sergenti and T. sinensis, especially in endemic countries.     

菜粉蝶绒茧蜂种群空间分布型及其应用

本文应用频次分布x~2检验法,Iwao法,Taylor幂法则以及其它聚集指标等方法,分析研究了菜粉蝶绒茧蜂种群(茧块)的空间分布型。结果表明:菜粉蝶绒茧蜂茧块在甘蓝上呈聚集分布,分布的基本成分是以个体群形式存在,个体群之间是随机的,茧块的聚集是由寄主等环境因素引起的。此外,利用分布型参数确定了理论抽样数及序贯抽样。     

豚草条纹叶(虫甲)的生物学特性

豚草条纹叶(虫甲)在北京地区年发生3代,以成虫入土越冬。越冬成虫于4月底至5月上出土活动。1、2、3代成虫分别于6月中下、7月下至8月初和9上中旬羽化出土。在26±1℃恒温条件下,雌雄成虫的寿命为82.5和67.8天。产卵前期16.5天,产卵期49.1天,一生产卵394.5粒。成虫一般仅在产卵后第30天前后出现一次明显的产卵高峰。卵、幼虫、蛹的发育历期分别为5.0、13.9和12.9天,在保温条件下,卵的孵化率最高,达92％。幼虫和蛹的存活率在16±1℃、26±1℃恒温及20～27℃室温条件下,差异不明显,最高78％,最低66.7％。     

除草剂在作物间的选择性

自1946年开始使用2，4-D以来，化学除草以其高效、经济、快速等优点而广泛应用于现代农业生产中。不同除草剂对不同作物具有杀伤性，只有了解除草剂对作物及杂草间选择性的决定因素，才能正确使用除草剂，达到“苗活草死”的效果。除草剂在不同植物间的选择性是由多种因素决定的，其表现方式也多种多样，本文概述了其选择性的主要决定因素。     

德宏局辖区边境口岸10年监测实蝇的结果与分析

利用德宏边境口岸10年的实蝇监测数据,从实蝇种类、种群数量和结构动态变化方面分析实蝇发生发展情况。结果如下:(1)10年间德宏地区共监测鉴定出13种实蝇,其中种群数量最多的是桔小实蝇、瓜实蝇和南瓜实蝇;(2)3种主要实蝇10年来发生数量呈上升态势,2009年增速最快,1年监测到的实蝇占10年总量的28.1%;(3)桔小实蝇已经成为德宏地区最大的优势种群,到2009年,它在3种主要实蝇中已占79.0%的绝对多数,增速也最快。