Methods to assay absorption of foliar applied nutrients and removal of unabsorbed residue 1

Various glues and adhesives were tested for their ability to remove the unabsorbed residue from drops of foliar applied radioactive nutrients to Ficus benjamina L. and Glycine max Merr. Most glues/adhesives adequately removed the unabsorbed residue from nutrients that dried forming a salt deposit on the leaf surface, but only 1.6–3.2% agar, Rhoplex 33, and Elmers Glue removed greater than 95% of the residue from nutrients that formed a moist hygroscopic residue on the leaf surface. Adding 25 mM KH₂PO₄ to 3.2% agar, followed by swabbing the treated area with lN HCl‐1% Liquinox detergent removed all unabsorbed residue from monovalent and divalent cationic an anionic nutrients including heavy metals. Methods to assay foliar absorption were compared by applying “Rb phosphate to leaves of intact plants, excised leaves and leaf discs. Excised leaves with their petiole or stem bases in water behaved similar to intact plants, whereas excised leaves or discs maintained in in a moist atmosphere behaved atypically due to slower and incomplete drying compared to intact plants.     

Conium maculatum (poison hemlock) toxicosis in a flock of range turkeys

Five 20-week-old tom turkeys from a flock of range turkeys were presented for examination; the flock had a history of salivation, tremors, paralysis, and increased mortality. Necropsy revealed numerous seeds identified as seeds from Conium maculatum (poison hemlock) within the crop, proventriculus, and gizzard. Histopathologic alterations were limited to catarrhal enteritis. Clinical signs of Conium maculatum toxicosis abated after the turkeys were removed from their range, which was infested with poison hemlock.     

Land degradation assessment in Southern Africa: integrating local and scientific knowledge bases

Recent attempts to address land degradation have seen calls for greater integration of scientific expertise with local knowledges. In this paper we investigate the potential for such combined understandings to enhance the accuracy, coverage and relevance of land degradation assessment. We followed a participatory approach, using methods from a variety of disciplines, to elicit potential land degradation indicators from communities in Botswana and Swaziland. These indicators were then assessed according to local and scientific understandings. We noted a significant overlap between scientific and local knowledges about land degradation in most instances. Where discrepancies occurred, the integrated participatory approach we used allows appropriate explanation to be reached, supporting the case that such an iterative process can lead to both accurate and relevant monitoring of land degradation. However, the incorporation of integrated knowledges into national policy has not been widespread in either country, suggesting that much greater efforts are required to institutionalise participatory land degradation assessment methodologies. Powerful, often neo‐Malthusian narratives of degradation continue to dominate policy discourse and limit the extent to which hybrid combined local and scientific knowledges can enhance land degradation assessment on a national and regional scale. Copyright © 2006 John Wiley & Sons, Ltd.     

Discovery of a viral NLR homolog that inhibits the inflammasome

The NLR (nucleotide binding and oligomerization, leucine-rich repeat) family of proteins senses microbial infections and activates the inflammasome, a multiprotein complex that promotes microbial clearance. Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to several human malignancies. We found that KSHV Orf63 is a viral homolog of human NLRP1. Orf63 blocked NLRP1-dependent innate immune responses, including caspase-1 activation and processing of interleukins IL-1β and IL-18. KSHV Orf63 interacted with NLRP1, NLRP3, and NOD2. Inhibition of Orf63 expression resulted in increased expression of IL-1β during the KSHV life cycle. Furthermore, inhibition of NLRP1 was necessary for efficient reactivation and generation of progeny virus. The viral homolog subverts the function of cellular NLRs, which suggests that modulation of NLR-mediated innate immunity is important for the lifelong persistence of herpesviruses.     

Insoluble fraction of buckwheat (Fagopyrum esculentum Moench) protein possessing cholesterol-binding properties that reduce micelle cholesterol solubility and uptake by Caco-2 cells

Buckwheat (Fagopyrum esculentum Moench) protein (BWP) exhibits hypocholesterolemic activity in several animal models by increasing fecal excretion of neutral and acidic sterols. In the current study, the ability of BWP to disrupt micelle cholesterol solubility by sequestration of cholesterol was investigated. When BWP (0.2%) was incubated with cholesterol and micelle lipid components prior to micelle formation, cholesterol solubility was reduced 40%. In contrast, cholesterol solubility was not decreased when BWP (0.2%) was incubated after micelle formation and incorporation of soluble cholesterol. Buckwheat flour, from which BWP was derived, had no significant effect on cholesterol solubility. Cholesterol uptake in Caco-2 cells from micelles made in the presence of BWP (0.2%) was reduced by 47, 36, 35, and 33% when compared with buckwheat flour, bovine serum albumin, casein, and gelatin, respectively. Reduction in cholesterol uptake in Caco-2 cells was dose-dependent, with maximum reductions at 0.1-0.4% BWP. In cholesterol-binding experiments, 83% of the cholesterol was associated with an insoluble BWP fraction, indicating strong cholesterol-binding capacity that disrupts solubility and uptake by Caco-2 cells.     

Resonating valence-bond ground state in a phenalenyl-based neutral radical conductor

An organic material composed of neutral free radicals based on the spirobiphenalenyl system exhibits a room temperature conductivity of 0.3 siemens per centimeter and a high-symmetry crystal structure. It displays the temperature-independent Pauli paramagnetism characteristic of a metal with a magnetic susceptibility that implies a density of states at the Fermi level of 15.5 states per electron volt per mole. Extended Hückel calculations indicate that the solid is a three-dimensional organic metal with a band width of approximately 0.5 electron volts. However, the compound shows activated conductivity (activation energy, 0.054 electron volts) and an optical energy gap of 0.34 electron volts. We argue that these apparently contradictory properties are best resolved in terms of the resonating valence-bond ground state originally suggested by Pauling, but with the modifications introduced by Anderson.     

Genital pathology of feral male goats

Genitalia from 1000 feral male goats derived from western Queensland and New South Wales were examined after slaughter at an abattoir and the prevalence of abnormalities determined. Ulcerative balanoposthitis, considered due to caprine herpesvirus infection, was observed in 11 animals (1.1%); acidophilic intranuclear inclusions were found in 7 of these. Other conditions included focal hypoplasia of seminiferous tubules in 2 bucks (0.2%), segmental aplasia of the epididymis (one buck, 0.1%), bulbourethral gland cysts with contained aggregations (33 bucks, 3.3%) and haemangiosarcoma of the bulbourethral gland in one animal. The low prevalence of several conditions such as spermatic granuloma, cryptorchidism, and testicular hypoplasia, was attributed largely to the fact that the bucks examined were horned so that the recognised association between genital abnormalities and polledness did not apply.