Impact of thermal stress exposure on seminal quality,antioxidant defence system,TNF-α and TIMP-3 in Ossimi ram

Heat stress (HS) occupies huge importance nowadays as it leads to severe economic losses especially in livestock. Preserving sheep against HS is one of the governmental scopes where it represents huge percentage of global ruminant. The present research was conducted to study semen quality, some stress and inflammatory markers in Ossimi rams under both hot and mild climatic conditions. The current study was conducted on selected 46 ram samples divided into two groups during summer and winter. Semen analysis, testosterone (TES), cortisol (COR) and blood glucose (BG) levels, and lipid and protein profiles were done. Concentrations of tumour necrosis factor alpha (TNF-α), tissue inhibitor of metalloproteinase-3 (TIMP-3), nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH) and specific activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were assessed. The results in summer compared to winter revealed significant elevation of total defects and number of dead sperms; however, there was reduction in sperm total motility and concentration and external epididymal tail duct diameter. Histological study of epididymal tail lumen exhibited azoospermia. Further, TES, TIMP-3 and GSH levels were decreased and COR, TNF-α, NO and MDA were raised. Specific activities of GPx and SOD were also declined. Additionally, there was a significant increase in concentrations of BG and lipid profiles except high-density lipoprotein. Our data concluded that there were new insights into TNF-α and TIMP-3 as biomarkers can be used in diagnosis of sheep suffering from HS, but further studies are recommended to do in future work about such aspect.     

Oil contamination in the Red sea environment

A survey of chronic oil pollution sources in the Red Sea and its gulves is given. The Red Sea is suffering from oil pollution along most of its coasts as well as in its offshore waters. Oil inputs from all sources in the Red Sea were estimated and compared with those in the world's oceans. While each km² in the world's oceans receives 9.17 kg yr^?1 of oil, each km² in the Red Sea receives 14.61 kg yr^?1. Oil inputs from refineries was also estimated for the Red Sea. It was found that each km² of the Red Sea receives about 13 times the quantity received by each km² in the world's oceans.     

Effects of subsoil compaction on yield and yield attributes of wheat in the sub-humid region of Pakistan

The prolonged use of vehicular traffic for farming creates subsoil compaction, which reduces crop yield and deteriorates the physical conditions of the soil. Field experiments were conducted during 2002–2003 and 2003–2004 in Pakistan to study subsoil compaction effects on soil bulk density, total porosity, yield and yield components of wheat. Soil compaction was artificially created at the start of the experiment using 7.0 t roller having length of 1.5 m and diameter of 1.22 m. Treatments consisted of T₁ = control (no compaction), T₂ = two passes of roller, T₃ = four passes of roller, T₄ = six passes of roller. The experiments were arranged in randomised complete block with four replications. Results indicated that subsoil compaction adversely affected the bulk density, total porosity of soil and root length during both the years. Soil compaction increased the bulk density (BD) from 1.37 for T₁ to 1.57, 1.61 and 1.72 Mg m⁻³ whereas decreased the total porosity from 47.3% for T₁ to 40.0, 37.4 and 34.5% for T₂, T₃ and T_4, respectively. Similarly grain yield decreased from 4141.7 for T₁ to 3912.8, 3364.5 and 3010.3 kg ha⁻¹ for T₂, T₃ and T_4, respectively. The deteriorating effect of compaction depended upon the degree of compaction. Subsoil compaction adversely affected the yield and yield attributes of wheat during both years of experiments. The subsoil compaction adversely affected soil physical conditions, which substantially decreased the yield of wheat. Therefore, appropriate measures of periodic chiselling, controlled traffic, conservation tillage, and incorporating of crops with deep tap root system in rotation cycle is necessary to minimize the risks of subsoil compaction.     

Gene expression of endothelin-1 and its receptors in the heart of broiler chickens with T(3)-induced pulmonary hypertension

To investigate the effect of T₃-induced pulmonary hypertension on endothelin (ET) production and genes expression of ET-1, ET_A and ET_B receptors (ET_AR and ET_BR) during rearing, semiquantitative RT-PCR and enzyme immunometric assay were performed in the heart ventricles and serum, respectively. The ET-1 and its receptor genes were expressed in the right and left ventricles of control and T₃-treated broilers at 12, 28 and 49 days of age. There were significant (P < 0.05) reductions of the relative amounts of ET-1 (in both ventricles) and ET_AR (in the right ventricle) mRNAs at 28 and 49 days of age, in T₃-treated broilers compared to controls. The relative amounts of ET_BR mRNA in the right and left ventricles did not significantly differ between control and T₃-treated broilers at any age. The serum level of ET was significantly (P < 0.05) increased in T₃-treated chickens at 28 and 49 days of age when compared with that of the control. It is concluded that ET-1, ET_AR and ET_BR genes are normally expressed in the heart ventricles of broilers. It is likely that increased serum level of ET and decreased ET-1/ET_AR genes expression in the ventricles are involved in the heart dysfunction of broiler chickens with developmental pulmonary hypertension.     

Assessment of Iranian chickpea (Cicer arietinum L.) germplasms for drought tolerance

Iran is one the most important centers of diversity for chickpea in south western Asia. Landraces are well adapted to local environmental conditions, and have evolved a range of morphological, phenological and physiological mechanisms to efficiently utilize the available water in a dry environment. In order to study some of these mechanisms, 150 genotypes of chickpea (Kabuli type) were evaluated using Augmented Designs for Preliminary Yield Trials under stress (Rainfed) and nonstress (Irrigated) conditions in Research Field of Mashhad College of Agriculture, Ferdowsi University of Mashhad, during 2005-2006 growing season. Then, based on the obtained results, four candidate genotypes for drought tolerance and two susceptible ones were evaluated in a pot experiment at open door situation in stressed (25% Field Capacity) and non-stressed (Field Capacity) conditions based on a factorial trial in Randomized Complete Block Design. There were positive and highly significant correlations between quantitative drought resistance indices such as MP, GMP, STI and HM with yield in stress and nonstress conditions. Also, there were positive and high significant correlations for SSI and DRI with yield in nonstress and stress conditions, respectively. Based on drought resistance indices and DRI, MCC544, MCC696 and MCC693 genotypes were superior to others, so they can be viewed as promising genotypes for drought resistance. These results were approved using three dimensional scatter graph and multivariate biplot graph. In stress condition, there were negative and high significant correlations between yield and days to flowering. Drought stress decreased leaf area per plant in all genotypes, significantly. In stressed and non-stressed conditions, leaf area in susceptible genotypes was more than that in tolerant genotypes, thus drought tolerance may be attributed to less transpiration and water loss because of smaller leaf size and reduced leaf area expansion in tolerant genotypes when drought stress develops.     

Humoral antibody responses to different H5N1 and H5N2 vaccination regimes: implications for the development of autogenously based vaccines

Whereas H5N1 vaccine and several H5N2 vaccines are commercially available and are used to control H5N1 outbreaks in some endemic countries, infections hit many vaccinated flocks. The following study was conducted to compare the efficacy of such vaccines and to assess their potential induction of antibodies against the haemagglutinin of local H5N1 isolate after single vaccination. The possible beneficiary effect of booster dose at different intervals was screened for both H5N1 vaccine as well as a selected H5N2 candidate. Differences in the serological immune response among native and cross breeds were also screened. No significant variations were detected between available commercial H5N1 and H5N2 vaccines after single vaccination. Two vaccination shots using H5N1 but not H5N2 vaccine were found to be superior to a single vaccination scheme, where chicks developed more conceivable antibody titers than in single vaccination program. There was considerable variation among chicken lines in the immune response to H5N1 vaccine: native breeds possessed the highest antibody titers as compared to other breeds.     

Characterization of Arcanobacterium abortisuis by phenotypic properties and by sequencing the 16S-23S rDNA intergenic spacer region

The present study was designed to characterize phenotypically and genotypically nine Arcanobacterium abortisuis strains collected from specimen of pigs in a period of nine years. All nine A. abortisuis strains and A. abortisuis reference strain DSM 19515 displayed a synergistic hemolytic reaction with Staphylococcus aureus β-hemolysin, Rhodococcus equi, and Arcanobacterium haemolyticum indicator strains and showed the typical biochemical properties of this species. The species identity could be confirmed by identification and sequencing of the 16S-23S rDNA intergenic spacer region (ISR), which appeared to be a useful target for genotypic characterization of this bacterial species. The A. abortisuis strains of the present study were isolated from specimen of pigs together with various other bacterial species indicating that the pathogenic importance of this newly described species remains to be elucidated.     

Ruminal excretion of sulfadimethoxine and sulfadimethyloxazole in goats and their influence on some enzyme activities and renal clearances

Five clinically health goats were injected with sulfadimethoxine and sulfadimethyloxazole in a single dose of 100 mg/kg b. wt. by intravenous route. Highest concentration levels of sulfadimethoxine and sulfadimethyloxazole in rumen were detected 1 hour following intravenous injection, then the concentration for both compounds declined at 12 and 8 hours post administration, respectively. In addition, both types of sulfonamide completely disappeared in ruminal fluid samples taken after 24 and 12 hours, respectively. The rate of acetylation for sulfadimethoxine and sulfadimethyloxazole were nearly similar and occurred to a high extent in ruminal fluid (22.95 and 23.72%, respectively). On the other hand, both tested drugs increased significantly the ruminal gas production from the first to eight hours after i.v. injection in goats. Changes in the serum enzyme activities (SGOT, SGPT and alkaline phosphatase) observed with sulfadimethoxine and sulfadimethyloxazole, and represented by a significant decrease in the activity of SGOT and SGPT level, alkaline phosphatase 4 hours sulfadimethoxine and in GOT/GPT ratio 24 and 48 hours after i.v. injection, respectively. The creatinine clearance was significantly decreased after 4 hours following the i.v. administration of sulfadimethoxine and sulfadimethyloxazole in goats.