Direct demonstration of milk as an element of archaeological economies

The stable carbon isotope (delta13C) compositions of individual fatty acid components of remnant fats preserved in archaeological pottery vessels show that dairying was a component of archaeological economies. Characteristic delta13C values arise from biases in the biosynthetic origins of the C18:0 fatty acids in milk and adipose fat. Milk and adipose fat from animals raised on similar pastures and fodders have distinct isotopic signatures.     

Effect of integrated nutrient management and crop diversification on productivity and nutrient balance sheet of rice-based cropping systems

A field study conducted for three crop years (June?–?July) from 1995?–?96 to 1997?–?98 at the Indian Agricultural Research Institute, New Delhi involving five rice-based cropping systems and six nutrient combinations indicated that rice?–?wheat?–?mungbean (RWM), rice?–?potato?–?mungbean (RPM), rice?–?rapeseed?–?mungbean (RRsM) and rice?–?clover (RC) cropping systems gave 0.7?–?1.0, 3.2?–?5.9, 0.2?–?2.2 and 1.5?–?3.6 t ha^?1 yr^?1, respectively, higher productivity and removed 7.9?–?22.6, 38.0?–?64.5, 53.0?–?61.8 and 51.4?–?66.1?kg ha^?1 yr^?1, respectively, more nitrogen, 2.3?–?7.1, 14.5?–?22.8, 3.8?–?7.2 and 17.3?–?21.7?kg ha^?1 yr^?1, respectively, more phosphorus and 1.6?–?11.4, 15.3?–?42.3, 8.2?–?22.7 and 40.8?–?57.8?kg ha^?1 yr^?1, respectively, more potassium than the rice?–?wheat (RW) cropping system which led to a net balance of +?151, +?58, ??153 and ??167?kg ha^?1 of nitrogen, +?13, ??27, ??8 and ??59?kg ha^?1 of phosphorus and ??549, ??551, ??558 and ??691?kg ha^?1 of potassium after three cycles of RWM, RPM, RRsM and RC cropping systems, respectively against a net balance of +?201, +?26 and ??533?kg ha^?1 of N, P and K, respectively in the RW cropping system. Application of FYM along with NPK in these cropping systems changed the negative balance of nitrogen and phosphorus into positive balance and reduced the negative balance of potassium by 151?–?378?kg ha^?1. Application of P and K fertilizers along with nitrogen also helped in arresting the negative balance of P and K under different rice based cropping systems. These results thus indicate that balanced use of NPK and FYM plays a major role for sustaining the productivity of a cropping system.     

Shearing during pregnancy — review of a policy to increase birthweight and survival of lambs in New Zealand pastoral farming systems

Over the past decade, lambing percentages have risen in conjunction with a rise in the percentage of multiple lambs born. Multiple-born lambs are smaller than their singleton counterparts and are particularly susceptible to starvation-exposure. Any technique that can increase the birthweight or thermoregulatory capability, or both, of otherwise lightweight lambs has the potential to substantially increase survival of multiple-born lambs.

In the United Kingdom under housed conditions, shearing during pregnancy has been shown to increase both the birthweight and thermoregulatory capability of newborn lambs. However, shearing during pregnancy under pastoral conditions has failed to consistently affect the newborn lamb’s thermoregulatory capability. In contrast, under New Zealand’s pastoral conditions shearing during pregnancy has been found to increase birthweight, but results have been inconsistent in both magnitude and birth-rank specificity. Increase in feed intake by the dam, types of shearing comb used and changes in gestation length do not explain the variation observed. When studies involving shearing during pregnancy are collated it becomes apparent that there are two criteria that must be met to achieve a birthweight response. Firstly, the dam must have the potential to respond and secondly, the dam must have the means to respond.

Any increase in lamb survival through shearing during pregnancy would be predominantly through an increase in birthweight of otherwise lightweight lambs. However, in the few studies conducted to date, an increase in birthweight has not resulted in a statistically significant increase in survival. For an increase in birthweight to have a positive effect on lamb survival, lambs must be otherwise destined to be born within a birthweight range in which survival rate is below optimum (<4.0 kg), and the increase in birthweight observed must move a significant proportion of otherwise lightweight lambs into a higher range of survival rate.     

Buffalo (Bubalus bubalis) Pre-antral Follicle Population and Ultrastructural Characterization of Antral Follicle Oocyte

The main objectives of the present study were to determine the ultrastructural modifications occurring in the oocyte during late folliculogenesis and to estimate pre-antral follicle population in buffalo. Half the collected ovaries were fixed and prepared for optic microscopy; the antral follicles from the other ovaries were measured and individually punctured. The cumulus–oocyte complexes (COCs) were processed for transmission electron microscopy. The number of pre-antral follicles in buffalo ovaries was estimated at 19 819 structures. Cumulus–oocyte complexes derived from 1-mm antral follicle had an eccentrical nucleus and compact corona radiata , ooplasm vilosities were fully embedded in zona pellucida (ZP) and a well-defined junction could be observed. Mitochondria were predominantly round and well distributed in ooplasm, as were small lipid vacuoles. In COCs derived from 2-mm antral follicles, the initial formation of perivitelline space was observed. The nucleus was peripherally located and the number of pleomorphic mitochondria increased. Cortical granules were clustered at oocyte periphery and lipid vacuoles increased in number and size. In COCs derived from 6-mm antral follicles, the organelles were located mainly in the perinuclear region. Golgi complexes and smooth endoplasmic reticulum (SER) were more developed. Mitochondria migrated to the cortical region and lipid vacuoles migrated to the medullar region. In COCs derived from 10-mm antral follicles, the lipid vacuoles coalesced and occupied the medullar region of the oocyte, together with a well-developed SER. Mitochondria were pleomorphic and located at the oocyte periphery. In conclusion, the morphological differences described in this paper could be responsible for some functional differences observed in in vitro embryo production and follicular dynamics for buffalo, when compared with cattle.     

Seasonal Variation of Plasminogen Activator Activity in Spermatozoa and Seminal Plasma of Boar,Buck, Bull and Stallion

Plasminogen activators (PA) are proteolytic enzymes present in the spermatozoa and seminal plasma of various species. They play a role in the binding of the spermatozoon and its penetration through the layers surrounding the oocyte. Plasminogen activator activity (PAA) is modulated by hormones that have a seasonal variation, such as testosterone and melatonin. The present study investigates the seasonal variation of PA activity in sperm extracts and seminal plasma of four farm animal species: boar, buck, bull and stallion. Semen samples were collected every second week during a 12‐month period and PAA was determined. With respect to sperm enzyme activity, the boar showed a peak from late January until the beginning of April, whereas the activity in the bull was at the highest levels from April until October and gradually declined during autumn and winter period. Plasminogen activator activity of stallion spermatozoa peaked during March and April, and remained low throughout the rest of the year, whereas in the buck sperm, PAA increased from late October until the end of January. No biologically significant variation was detected regarding the seminal PAA activity in any of the species studied. While seasonality of reproduction is typically studied from the female perspective, the present data provide compelling information about a factor that may affect the reproductive ability of the male.