全文获取类型
收费全文 | 132496篇 |
免费 | 7269篇 |
国内免费 | 93篇 |
专业分类
林业 | 5381篇 |
农学 | 4270篇 |
基础科学 | 831篇 |
17038篇 | |
综合类 | 21485篇 |
农作物 | 5397篇 |
水产渔业 | 6688篇 |
畜牧兽医 | 67973篇 |
园艺 | 1806篇 |
植物保护 | 8989篇 |
出版年
2020年 | 1178篇 |
2019年 | 1375篇 |
2018年 | 2032篇 |
2017年 | 2331篇 |
2016年 | 2095篇 |
2015年 | 1742篇 |
2014年 | 2225篇 |
2013年 | 5293篇 |
2012年 | 4114篇 |
2011年 | 5095篇 |
2010年 | 3245篇 |
2009年 | 3389篇 |
2008年 | 4973篇 |
2007年 | 4669篇 |
2006年 | 4356篇 |
2005年 | 4089篇 |
2004年 | 3852篇 |
2003年 | 3975篇 |
2002年 | 3544篇 |
2001年 | 4150篇 |
2000年 | 4252篇 |
1999年 | 3372篇 |
1998年 | 1474篇 |
1997年 | 1387篇 |
1996年 | 1288篇 |
1995年 | 1530篇 |
1994年 | 1358篇 |
1993年 | 1231篇 |
1992年 | 2584篇 |
1991年 | 2701篇 |
1990年 | 2591篇 |
1989年 | 2612篇 |
1988年 | 2368篇 |
1987年 | 2348篇 |
1986年 | 2427篇 |
1985年 | 2340篇 |
1984年 | 1881篇 |
1983年 | 1678篇 |
1982年 | 1149篇 |
1979年 | 1748篇 |
1978年 | 1398篇 |
1977年 | 1151篇 |
1976年 | 1212篇 |
1975年 | 1292篇 |
1974年 | 1500篇 |
1973年 | 1515篇 |
1972年 | 1406篇 |
1971年 | 1304篇 |
1970年 | 1251篇 |
1969年 | 1283篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
981.
Aerobic bacterial populations were studied on the distal hair coat and at the skin surface of the shoulder, rump and abdomen of 10 healthy dogs. Coagulase negative staphylococci (CNS) were more frequently isolated from the hair than the skin at the shoulder and rump. There was no difference in the isolation rate of coagulase positive staphylococci (CPS) (Staphylococcus intermedius) between the hair and skin. Total skin counts were greatest on the abdomen whereas CNS counts from the hair were least at this site. There were no differences between CPS counts at the three sites on either hair or skin. The populations on the relatively unfavourable microenvironment of the distal hair may represent contamination rather than colonisation. The low populations of CPS at the skin surface also indicate contamination or transient colonisation rather than true resident status. 相似文献
982.
Geelen SN Jansen WL Sloet van Oldruitenborgh-Oosterbaan MM Breukink HJ Beynen AC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(5):478-481
The aim of this study was to establish the dose-response relationship between fat intake and heparin-released plasma lipoprotein lipase (LPL) activity in horses. Eight mature trotters were fed 4 rations with different fat levels (3.0, 5.0, 7.7, or 10.8% fat in the dry matter) according to a 4 X 4 Latin square design. The experimental rations consisted of hay and different concentrates; the concentrates and hay were given in a 3:1 ratio on an energy basis. Soybean oil was added to the concentrates at the expense of isoenergetic amounts of glucose. Blood samples were taken at the end of each dietary period, which lasted 3 weeks. Fat feeding was found to increase heparin-released plasma LPL activity in a dose-dependent fashion. When the data from this study and previous studies were combined it was calculated that an increase in fat intake by 1 g/kg dry matter is associated with an increase in LPL activity by 0.98 micromol fatty acid released-mL(-1) x h(-1). Fat feeding raised the plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, and phospholipids. Diet did not have a statistically significant effect on plasma triacylglycerol concentrations. The results are discussed in the light of the possible enhancing effect of fat feeding on the oxidative capacity of skeletal muscle. 相似文献
983.
M. D. Pintore L. Ceballos B. Iulini L. Tomassone A. Pautasso D. Corbellini F. Rizzo M. L. Mandola M. Bardelli S. Peletto P. L. Acutis A. Mannelli C. Casalone 《Zoonoses and public health》2015,62(5):365-374
Following reports of human cases of Lyme borreliosis from the Ossola Valley, a mountainous area of Piemonte, north‐western Italy, the abundance and altitudinal distribution of ticks, and infection of these vectors with Borrelia burgdorferi sensu lato were evaluated. A total of 1662 host‐seeking Ixodes ricinus were collected by dragging from April to September 2011 at locations between 400 and 1450 m above sea level. Additional 104 I. ricinus were collected from 35 hunted wild animals (4 chamois, 8 roe deer, 23 red deer). Tick density, expressed as the number of ticks per 100 m2, resulted highly variable among different areas, ranging from 0 to 105 larvae and from 0 to 22 nymphs. A sample of 352 ticks (327 from dragging and 25 from wild animals) was screened by a PCR assay targeting a fragment of the 16S rRNA gene of B. burgdorferi s.l. Positive samples were confirmed with a PCR assay specific for the 5S‐23S rRNA intergenic spacer region and sequenced. Four genospecies were found: B. afzelii (prevalence 4.0%), B. lusitaniae (4.0%), B. garinii (1.5%) and B. valaisiana (0.3%). Phylogenetic analysis based on the ospC gene showed that most of the Borrelia strains from pathogenic genospecies had the potential for human infection and for invasion of secondary body sites. 相似文献
984.
Ecotypic variations in leaf conductance, soil-to-leaf hydraulic conductance, components of tissue water potential, hydraulic architecture parameters and xylem embolism were examined in greenhouse-grown two-year-old Aleppo pine (Pinus halepensis Mill.) seedlings from six origins representing the geographic range of the species in Italy. Cortical resin composition of the seedlings was also determined. Measurements were made on well-watered seedlings and on seedlings subjected to recurring severe drought. Drought-stressed seedlings had lower mean leaf conductances, transpiration rates and soil-to-leaf hydraulic conductances than well-watered seedlings. They also exhibited more negative osmotic potentials, higher relative water deficit at incipient plasmolysis, but a similar maximum modulus of elasticity. Drought-stressed seedlings showed a higher degree of xylem embolism, a lower Huber value, lower leaf specific conductivity and lower specific conductivity than well-watered seedlings. Drought-stressed seedlings of provenances from more xeric habitats (Tremiti, Porto Pino and Mottola) had greater leaf conductances, transpiration rates and soil-to-leaf hydraulic conductances than drought-stressed seedlings of provenances from more mesic habitats (Imperia, Otricoli and Vico del Gargano). They also showed higher osmotic adjustment and a lower degree of xylem embolism. Among provenances, there were no significant differences in hydraulic architecture parameters in response to the drought treatment; however, Tremiti and Porto Pino seedlings displayed smaller drought-induced reductions in specific conductivity and leaf specific conductivity, respectively, than seedlings from other provenances. These differences suggest that seedlings from xeric provenances, especially Tremiti, have greater resistance to desiccation than seedlings from mesic provenances. No clear association was found between terpene variability and the other traits investigated, although terpene composition was related to the geographical distribution of the provenances. We conclude that the drought-tolerance responses of Tremiti make it a more suitable provenance than the others for establishment on sites prone to severe soil water deficits. 相似文献
985.
986.
Freeman RG Grabar KC Allison KJ Bright RM Davis JA Guthrie AP Hommer MB Jackson MA Smith PC Walter DG Natan MJ 《Science (New York, N.Y.)》1995,267(5204):1629-1632
The self-assembly of monodisperse gold and silver colloid particles into monolayers on polymer-coated substrates yields macroscopic surfaces that are highly active for surface-enhanced Raman scattering (SERS). Particles are bound to the substrate through multiple bonds between the colloidal metal and functional groups on the polymer such as cyanide (CN), amine (NH(2)), and thiol (SH). Surface evolution, which can be followed in real time by ultraviolet-visible spectroscopy and SERS, can be controlled to yield high reproducibility on both the nanometer and the centimeter scales. On conducting substrates, colloid monolayers are electrochemically addressable and behave like a collection of closely spaced microelectrodes. These favorable properties and the ease of monolayer construction suggest a widespread use for metal colloid-based substrates. 相似文献
987.
Hewitt SA Brisson BA Sinclair MD Foster RA Swayne SL 《Journal of the American Veterinary Medical Association》2004,225(1):65-71
OBJECTIVE: To evaluate feasibility of performing laparoscopic-assisted placement of a jejunostomy feeding tube (J-tube) and compare complications associated with placement, short-term feedings, and medium-term healing with surgically placed tubes in dogs. DESIGN: Prospective study. ANIMALS: 15 healthy mixed-breed dogs. PROCEDURES: Dogs were randomly allocated to undergo open surgical or laparoscopic-assisted J-tube placement. Required nutrients were administered by a combination of enteric and oral feeding while monitoring for complications. Radiographic contrast studies documented tube direction and location, altered motility, or evidence of stricture. RESULTS: Jejunostomy tubes were successfully placed in the correct location and direction in all dogs. In the laparoscopic group, the ileum was initially selected in 2 dogs, 2 dogs developed moderate hemorrhage at a portal site, and 2 J-tubes kinked during placement but were successfully readjusted postoperatively. All dogs tolerated postoperative feedings. All dogs developed minor ostomy site inflammation, and 1 dog developed bile-induced dermatitis at the ostomy site. Despite mild, transient neutrophilia, no significant difference was noted in WBC counts between groups. No dog had altered gastric motility or evidence of stricture, although the jejunopexy site remained identifiable in several dogs at 30 days. CONCLUSIONS AND CLINICAL RELEVANCE: Requirements for successful J-tube placement were met by use of a laparoscopic-assisted technique, and postoperative complications were mild and comparable to those seen with surgical placement. Laparoscopic-assisted J-tube placement compares favorably to surgical placement in healthy dogs and should be considered as an option for dogs requiring enterostomy feeding but not requiring a celiotomy for other reasons. 相似文献
988.
O. O. Odedara J. d’A. Hughes A. C. Odebode B. O. Odu 《Journal of General Plant Pathology》2008,74(4):322-325
Leaf samples of Lablab purpureus collected from two agroecological zones of Nigeria—the northern guinea savanna zone (NGSZ) and the derived savanna zone (DSZ)—were
infected with viruses when serologically indexed against available antisera. Approximately 31.1 and 81.1% of the leaf samples
collected from the NGSZ and DSZ, respectively, were infected. Seven viruses were found: Bean common mosaic virus (BCMV), Cowpea aphid-borne mosaic virus (CABMV), Cucumber mosaic virus (CMV), Cowpea mottle virus (CPMoV), Cowpea severe mosaic virus (CPSMV), Southern bean mosaic virus (SBMV) and Tobacco mosaic virus (TMV) were detected from samples collected from NGSZ, while CMV, CPMoV, Cowpea mosaic virus (CPMV) and CPSMV were detected from samples from DSZ. 相似文献
989.
Surgical correction of gastro‐oesophageal intussusception with bilateral incisional gastropexy in three dogs 下载免费PDF全文
Three dogs presented for evaluation of acute onset tachypnoea and dyspnoea following episodes of vomiting and/or regurgitation. Thoracic radiographs were suggestive of a gastro‐oesophageal intussusception in all three dogs; one dog also showed evidence of aspiration pneumonia. All three dogs underwent surgical correction with a bilateral incisional gastropexy. All dogs recovered from anaesthesia uneventfully and were discharged from the hospital 3 days after presentation. Persistent megaoesophagus was evident in all three dogs, and they are being chronically managed with a strict feeding regime and pro‐motility agents. 相似文献
990.
Cold tolerance during juvenile development in sorghum: a comparative analysis by genomewide association and linkage mapping 下载免费PDF全文
Karin Fiedler Wubishet A. Bekele Claudia Matschegewski Rod Snowdon Silke Wieckhorst Arndt Zacharias Ralf Uptmoor 《Plant Breeding》2016,135(5):598-606
Improved cold tolerance during the juvenile phase is a major breeding goal to develop new sorghum cultivars suitable as an alternative energy crop in temperate regions. The objectives of this study were to identify marker‐trait associations for cold tolerance in a sorghum diversity panel fingerprinted with 2620 single nucleotide polymorphism (SNP) markers and to detect quantitative trait loci (QTL) in two F2:3 populations. Traits of interest were dry matter growth rate (DMGR), leaf appearance rate (LAR), chlorophyll content (SPAD) and chlorophyll fluorescence (Fv′/Fm′ and ФPSII) in relation to temperature. The association panel comprised 194 genotypes, while the F2:3 populations consisted of 80 and 92 genotypes. All populations were tested under controlled conditions in a minimum of four temperature regimes ranging from 9.4°C to 20.8°C. QTL were identified for means across environments and regression parameters describing temperature effects. Several marker‐trait associations for mean (m) DMGR, base temperature (Tb) of SPAD and ФPSII and temperature effect on LAR were validated by QTL detected in population 1 or 2. Promising QTL regions were found on chromosomes SBI‐01, SBI‐02, SBI‐03, SBI‐04, SBI‐06 and SBI‐09, among them genomic regions where candidate genes involved in the C‐repeat binding pathway or encoding cold‐shock proteins are located. 相似文献