Assessment of the potential toxicity of a poison for rabbits, pindone (2-pivalyl 1, 3 indandione), to domestic animals

The toxicity of pindone, a rabbit poison, to horses, cattle, goats, chickens, dogs and cats was investigated, using extension of prothrombin time (PT) as an index of poisoning. The daily dose of pindone, administered for 5 days, ranged from 0.3 mg/kg for dogs to 2.5 mg/kg for chickens. This range of dose rates was considered to be indicative of the worst possible case that could arise following a campaign of baiting for rabbits. Although significant elevations in PT (more than double baseline values) were noted in all species other than horses, clinical signs of anticoagulant poisoning were not observed in any of the species tested. From the observed PT, cattle and cats appeared to be the most susceptible, and horses the least susceptible, to pindone toxicity. The half-lives of the elevated PT were calculated as 3.1 days for cattle, 2.8 days for goats and chickens, 1.9 days for horses and dogs and less than one day for cats. It is proposed that these half-lives can be used as a guide for determining the duration of treatment of pindone-affected animals.     

Effects of Biochar and Marble mud on Mine Waste Properties to Reclaim Tailing Ponds

The effects of biochar addition in improving soil physical properties are not clearly understood in mining tailings. The objective of this study was to determine the effects of three different types of biochars, in addition to marble mud (MM) and their mixtures, on the structural stability and water retention of mine wastes in Cartagena, Spain. Biochars were produced at 500 °C from pig manure (PM), cotton (Gossipium hirsutum L.) crop residues (CR) and municipal solid waste (MSW). Biochars were added to the mine waste (MW) along with MM and a control (no amendments added). These mixtures were incubated in cores for 90 days (25 °C). PM and CR mixed with MM decreased soil bulk density (from 0 · 98 g cm^‐3 to 0 · 89 and 0 · 84 g cm⁻³, respectively). Amendments had no significant effect on total porosity whereas they increased gas diffusion by 100%, except for MSW. MM improved the plant available water from 0 · 59 to 2 · 56 cm as its combination with biochars, extremely relevant in water scarce climates. The micropores were likely replaced by mesopores when application of PM, CR, MM, and biochars + MM and they improved water retention. Total carbon (TC) and total nitrogen (TN) increased by using biochars and MM and no significant effects were assessed on aggregates. In general, MM mixed with PM and CR derived biochar improved the structural stability and exhibited a strong impact in reclaiming physical quality on mine tailings. Copyright © 2016 John Wiley & Sons, Ltd.     

Anaerobic digestion of wastewater derived from the pressing of orange peel generated in orange juice production

A study of the anaerobic digestion of wastewater from the pressing of orange peel generated in orange juice production was carried out in a laboratory-scale completely stirred tank reactor at mesophilic temperature (37 degrees C). Prior to anaerobic treatment the raw wastewater was subjected to physicochemical treatment using aluminum sulfate as a flocculant and to pH reduction using a solution of sulfuric acid. The reactor was batch fed at COD loads of 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, and 5.0 g of COD. The process was very stable for all of the loads studied, with mean pH and alkalinity values of 7.5 and 3220 mg of CaCO3/L, respectively. The anaerobic digestion of this substrate was found to follow a first-order kinetic model, from which the specific rate constants for methane production, K(G), were determined. The K(G) values decreased considerably from 0.0672 to 0.0078 L/(g h) when the COD load increased from 1.5 to 5.0 g of COD, indicating an inhibition phenomenon in the system studied. The proposed model predicted the behavior of the reactor very accurately, showing deviations of <5% between the experimental and theoretical values of methane production. The methane yield coefficient was found to be 295 mL of CH4 STP/g of COD removed, whereas the mean biodegradability of the substrate (TOC) was 88.2%. A first-order kinetic model for substrate (TOC) consumption allowed determination of the specific rate constants for substrate uptake, K(C), which also decreased with increasing loading, confirming the above-mentioned inhibition process. Finally, the evolution of the individual volatile fatty acid concentrations (acetic, C2; propionic, C3; butyric, C4; isobutyric, iC4; valeric, C5; isovaleric, iC5; and caproic, C6) with digestion time for all loads used was also studied. The main acids generated were acetic and propionic for all loads studied, facilitating the conversion into methane.     

Kinetic study of the activation of banana juice enzymatic browning by the addition of maltosyl-beta-cyclodextrin

In recent years, the use of cyclodextrins (CDs) as antibrowning agents in fruit juices has received growning attention. However, there has been no detailed study of the behavior of these molecules as substances, which can lead to the darkening of foods. In this paper, when the color of fresh banana juice was evaluated in the presence of different CDs, the evolution of several color parameters was the opposite of that observed in other fruit juices. Moreover, a kinetic model based on the complexation by CDs of the natural browning inhibitors present in banana is developed for the first time to clarify the enzymatic browning activation of banana juice. Finally, the apparent complexation constant between the natural polyphenoloxidase inhibitors present in banana juice and maltosyl-beta-CD was calculated (Kci = 27.026 +/- 0.212 mM (-1)).     

Proteomic identification of actin-derived oligopeptides in dry-cured ham

An intense proteolysis of muscle proteins, mainly due to the action of endogenous proteolytic enzymes, has been reported to occur during the processing of dry-cured ham. This gives rise to an important generation of free amino acids and peptides of small size that contribute directly or indirectly to flavor characteristics of the final product. The nature and properties of the free amino acids generated during postmortem proteolysis have been well established. However, little is known about the identity of peptides generated during the processing of dry-cured ham. In the present paper, we describe the isolation (by ethanol precipitation followed by size exclusion and reverse phase chromatographies) and identification (by matrix-assisted laser desorption/ionization time-of-flight MS and collision-induced dissociation MS/MS) in a Spanish dry-cured ham of the following four oligopeptides: (A) TKQEYDEAGPSIVHR, (B) ITKQEYDEAGPSIVHRK, (C) DSGDGVTHNVPIYE, and (D) DSGDGVTHNVPIYEG. This is the first time that these peptide fragments have been reported in dry-cured ham at the end of processing. Sequence homology analysis revealed that the four peptides originated from muscle actin, indicating an extensive hydrolysis of this protein during dry-curing. Some of the cleavage sites corresponding to these fragments in actin were reproduced by other authors through the incubation of this myofibrillar protein in the presence of cathepsin D (EC 3.4.23.5), thus supporting a relevant action of this enzyme during the processing of dry-cured ham.     

Fertility of sows mated on different days of the week

The farrowing rates resulting from matings on different days of the week were determined for a 2800-sow intensive piggery in both 1983 and 1984. The results showed that sows mated early in the week achieved a significantly higher farrowing rate than sows mated late in the week (P less than 0.0001). During the seasonal (summer to autumn) infertility period, Wednesday matings resulted in a poor farrowing rate whereas for the rest of the year farrowing rates following Wednesday matings were similar to those for Sunday Monday and Tuesday matings. Consistent results were obtained for data from both years. It is proposed that the stress of regrouping unmated sows on Wednesday to accommodate newly weaned sows is responsible for this decrease in fertility. This study illustrates how detailed analysis of farrowing rates on a day of mating basis can identify a problem which is otherwise masked by weekly data.     

Effect of spinal cord transection on feather release in the slaughtered broiler

1. Transection at the level of the first cervical vertebra causes a marked loosening of feather‐skin connection, although less than that found in the living animal. This effect may be seen even when transection is carried out 2.lb5 min after bleeding.

2. Cuts performed in other levels had little or no effect.     

罗马尼亚沙棘资源建设和利用状况

沙棘,亚欧种,1753年植物学家Karl von Linné发现了沙棘并分类为"植物志"的第1023种.沙棘(Hippophae rhamnoides)被认为是希腊语"闪闪发亮的马"的语源.罗马尼亚的专家论证了沙棘对动物健康的影响,特别是皮肤方面,沙棘还有着消除肠内病菌的作用.     

Severity and persistence of footrot in Merino sheep experimentally infected with a protease thermostable strain of Dichelobacter nodosus at five sites

Objective To test the hypothesis that ovine footrot associated with a thermostable protease strain of Dichelobacter nodosus undergoes self cure or is sustained as an annually recurring disease, depending on the environment.
Design and procedure Forty Merino sheep from a single blood line were infected with a protease thermostable strain of D nodosus a t each of five sites in Western Australia. Footrot lesions and microscopic evidence of D nodosus were recorded every fortnight for 2.5 years, supplemented by laboratory culture. Rainfall, soil and air temperature, pasture quantity and composition and soil types were also recorded. Flocks that apparently self cured were relocated to a more favourable site for footrot in the final spring season.
Results The maximum prevalence of feet with clinical footrot lesions was 80.6, 1.3, 14.4, 3.8 and 88.1% at the five sites. Severe footrot occurred for three consecutive spring seasons at one site that had clay loam soil and at least 3500 kg/ha total pasture dry matter annually. However, the infection was asymptomatic for up to 10 weeks between outbreaks. D nodosus was isolated from flocks for 2.5 years at only two sites, although there was microscopic evidence of the organism at other sites in the final year. A thermolabile variant (strain U6) of D nodosus was isolated from the two sites where footrot persisted.
Conclusion Depending on time and location, ovine footrot induced by a protease thermostable strain of D nodosus either self cured or persisted as annual outbreaks interspersed with periods of asymptomatic infection.