Isolation,Identification and Complete Genomic Sequence Analysis of Goose Astrovirus

From October 2017 to May 2019, 67 samples of typical goslings gout were collected from Guangdong, Sichuan, Hebei, Shandong, Anhui, Liaoning provinces and other vast goose-raising areas in China. The results showed that goose astrovirus was detected in all samples and about 94.03% of the samples were infected by two different kinds of goose astrovirus. The pathogenicity of goose astrovirus FLX variant strain (named SCCD) which could stably proliferate in goose embryos and kill 10-day-old goose embryos was more virulent than goose astrovirus FLX strain. The new type of goose astrovirus strain (named SDPD) could't stably proliferate in goose embryos and SPF chicken embryos. The whole genome nucleotides homology of goose astrovirus FLX strain with goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain were 89.7% and 58.1%, the amino acids homology of ORF1b gene were 98.4% and 61.0%, the amino acids homology of ORF2 gene were 81.0% and 42.5%, respectively. The newly isolated goose astrovirus strains were inoculated into 1-day-old healthy goslings, the results showed that although the goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain could cause the death of goslings and the changes of hepatitis, kidney swelling and weight loss, none of the goslings showed typical gout characteristics (urate deposition in the organs of the body), however, 44% of goslings were characterized with typical gout which was consistent with the symptoms of natural disease after coinfected by the two goose astrovirus strains. Therefore, the pathogenic agents of goslings gout may be two different kinds of goose astrovirus, namely the new type of goose astrovirus and the variant of goose astrovirus FLX.     

Effect of intestinal endotoxemia on hepatic energy metabolism in acute liver failure

AIM:To study the effect of intestinal endotoxemia(IETM) on hepatic energy metabolism in acute liver failure. METHODS:Intoxication by thioacetamide (TAA) was used to establish rat model of acute liver injury.Ketone body(acetoacetate and β-hydroxybutyrate) in arterial blood and ATP content of hepatocellular mitochondria were determined by using enzymatic fluorimetric micromethod.Colectomy was adopted in observing the changes in plasma endotoxin content and serum alanine aminotransferase (ALT) activity. RESULTS:In the TAA group,plasma endotoxin content and serum ALT activity were all significantly higher than those in the control group(P＜0.01),arterial ketone body ratio of acetoacetate to β-hydroxybutyrate (AKBR) decreased below 0.4,total ketone body in arterial blood was significantly lower than that in the control group(P＜0.01).In the TAA+colectomy group,there was no endotoxemia to be found,ATP content of hepatocellular mitochondria was significantly higher than that in the TAA group(P＜0.01), though serum ALT activity was higher than that in the control group(P＜0.05),but significantly lower than that in the TAA group(P＜0.01). CONCLUSION:IETM played a key role in the occurrence of acute liver failure,hepatic dysfunction might be caused by IETM through damaging hepatic energy metabolism.     

我国水稻上的一种新的細菌性病害

 1959年5月間,在杭州早稻生长的前期,叶片和叶鞘上发現一种新的病害。病斑最初暗綠色,以后呈黑褐色,带棱形,病斑的外形与稻瘟病有些相似,严重时引起稻株的枯死。     

羊驼主要病毒性传染病的诊断与防治

概述了羊驼主要病毒性传染病的流行病学、临床症状、病理变化及诊断与防治。为羊驼病毒性传染病的研究与防治提供理论依据。     

草业琐谈之三十四胡杨泪

自从1950年来到西北,胡杨就是我的老相识了.胡杨的坚韧和壮美令人折服.胡杨(还有牦牛,这里暂且不说)是我心中的图腾,我对它顶礼膜拜.     

草业琐谈之四十一越南琐忆——感人的友情

回忆越南,最想表达的是中越两国人民的友情.但偏偏最后来写,因为内容太丰富,再三咀嚼回味,也难以理出个头绪来.     

新疆南北疆区域经济发展差异预警研究

以1993年和2009年两个时间断面上的统计数据为依据,采用统计学方法对2000年以来新疆南北疆区域经济差异状况进行比较分析。结果发现:新疆南北疆区域经济发展存在明显差异,北疆经济发展水平明显优于南疆;在此基础上对南北疆经济差异程度进行了预警研究,研究发现南北疆经济绝对差异已经超出警戒线,而相对差异也已接近警戒线水平。最后,对此提出了相应的政策建议。     

1960-2009年大连市气候暖干化研究

利用大连市1960-2009年气温和降水两个主要气象要素值的基本观测资料,运用线性倾向估计和GIS空间分析方法,探讨了大连气候的年际时空变化,同时利用Mann-Kendall统计检验和Morlet小波分析对其气候的年代际和周期性变化进行了分析。结果表明:近50a来大连市气候暖干化日趋明显,年平均气温以0.28℃/10a的速率显著上升,并且在1991年发生突变进入异常增暖期,空间上增暖趋势大致呈由南向东北逐渐减弱的态势;降水量基本经历了"减少-增加-减少-增加"的变化过程,总体上呈下降趋势,在1972年降水量由偏多向偏少转变,东北和西南地区降幅较大,腹地最小。此外,气候的暖干化趋势对"自然-社会-经济"复合系统造成的影响应引起重视。     

高校大学生阅读方式与习惯模式的研究

通过对高校在校大学生阅读方式和阅读习惯的问卷调查,并对所得数据进行分析找到对策,从而发挥图书馆主阵地的作用,对大学生人文阅读进行不同层次、不同级别的指导,让高校图书馆的职能更好的发挥,服务更加深化。     

Effects of Zuogui pill-medicated serum on proliferation and differentiation of MC3T3-E1 cells via ERK/TGF-β/Smads signaling cascade

AIM:To study the effects of Zuogui pill(ZG)-medicated serum on the proliferation and differentiation of MC3T3-E1 cells via ERK/TGF-β/Smads signaling pathway. METHODS:Using Premarin(conjugated estrogens tablets) as a positive control, the SD female rats were fed with high-, medium- or low-dose of ZG suspension. ZG-medicated serum was separated from abdominal aortic blood 7 d after feeding of ZG. MTT assay was applied to test the effect of ZG-medicated serum on the viability of MC3T3-E1 cells. The production of alkaline phosphatase(ALP) was detected by a modified calcium and cobalt dyeing method. The calcified nodules were observed by the method of alizarin red staining. The levels of core binding factor α1(Cbfα1) and collagen type I(Col I) protein were analyzed by Western blotting. The mRNA expression of TGF-β₁, Smad4 and Smad2 was measured by real-time RT-PCR. RESULTS:ZG-medicated serum promoted the proliferation of MC3T3-E1 cells in a dose-and time-dependent manner. Compared with other groups, treatment with 15% ZG(low dose) for 48 h increased the proliferation of MC3T3-E1 cells significantly. The protein levels of ALP, Cbfα1 and Col I,the calcified nodules, and the mRNA expression of TGF-β₁, Smad4 and Smad2 in MC3T3-E1 cells were all significantly increased after treatment with ZG-medicated serum. After the addition of PD98059(a specific blocker of ERK1/2 signaling pathway), all those were down-regulated except for mRNA expression of TGF-β₁. CONCLUSION:ZG regulates MC3T3-E1 cell proliferation and differentiation via the intervention of ERK/TGF-β/Smads signaling cascade, which may be one of the mechanisms that ZG effectively prevents and treats osteoporosis.