Cx43 is involved in electrical remodeling of atrial myocytes through regulating L-type calcium current

AIM: To investigate whether the association of connexin 43(Cx43) and L-type calcium channel involved in the pathogenesis of atrial fibrillation (AF). METHODS: The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue. The co-localization of Cx43 and L -type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated. RESULTS: The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients. In cultured atrium-derived myocytes (HL-1 cells), knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channel α1c subunit, as well as L-type calcium current. Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed. CONCLUSION: The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation.     

青田县海溪乡柑橘黄龙病防控策略分析

海溪乡是青田县温州蜜柑的主产区,栽培柑橘面积273.8hm2,全乡2350户农民均以柑橘为生,人均种植柑橘面积达0.03hm2.2002年对全县柑橘黄龙病普查发现,黄龙病感病面积达10.88hm2,12324株.如何有效地防控柑橘黄龙病已是摆在全乡橘农及政府部门的头等大事.海溪乡柑橘黄龙病防控工作在省科技特派员陈友清、李丽的大力支持下,通过全乡橘农和乡镇党委、政府通力合作,已获得了相当不错的成效.为了有效防治、控制我省柑橘黄龙病发生和蔓延,促进全省柑橘产业化可持续继发展,全面提升我省三农经济产业,现将全乡柑橘黄龙病防控策略分析如下,供大家在黄龙病防控工作中参考.……     

除草剂变量施药技术的研究现状与进展

除草剂的田间大面积均匀喷洒不仅造成了浪费,而且加剧了环境污染,作为精确农业关键技术的除草剂的变量施药能够精确地对准杂草喷洒除草剂,从而有效地减少除草剂用量,降低了农业生产成本、减轻了环境污染压力。文章介绍了基于颜色、形状、纹理、光谱、位置特征的计算机视觉杂草识别技术;给出了变量施药技术的概念和介绍了变量施药技术的国内和国外的研究概况,并探讨了该技术的实施难点和未来研究发展方向。     

漂烫处理和速冻处理对白芦笋营养成分的影响

研究了不同漂烫条件和速冻处理对芦笋过氧化物酶(POD)活性及营养成分的影响。结果表明:漂烫处理和速冻处理造成一定量的蛋白质和氨基酸的损失。95℃条件下,漂烫2.5 min能显著抑制POD活性,较好地保存芦笋的外观品质,较长时间保持速冻芦笋的营养成分。     

充N2包装对猕猴桃鲜果切片品质和生理效应的影响

以美味猕猴桃“秦美”果实为试材,研究了充N2包装对其鲜切后果实呼吸速率、乙烯释放速率、丙二醛(MDA)含量等生理指标和果实硬度、可溶性固形物(TSS)含量、维生素C含量、可滴定酸含量等品质指标的影响。结果表明,充N2处理后,果实的呼吸速率和乙烯释放速率受到抑制,呼吸速率高峰比对照延迟了4 d,乙烯释放速率高峰比对照推迟了8 d;充N2包装降低了鲜切猕猴桃果实丙二醛(MDA)的含量,抑制了Vc的降解,但对果实可滴定酸含量没有明显影响;充N2包装可以抑制鲜切猕猴桃果实硬度的下降速度,推迟果实的软化,同时TSS含量的上升速度也比对照缓慢。综合各项指标分析认为,充N2包装对提高鲜切猕猴桃果片品质以及延长其货架期有显著效果。     

车八岭国家级自然保护区空气负离子水平研究

在实地测定车八岭国家级自然保护区空气负离子浓度的基础上，对不同样点的空气负离子浓度与环境多元变量的关系进行分析，结果表明：溪边、瀑布边以及接近水面的样点空气负离子浓度较高，森林土壤和植被对空气负离子浓度影响的正效应明显，建议保护区修建专用的空气负离子健康道，提供更多的休憩、娱乐和保健场所。     

酒糟单细胞蛋白饲料生产技术研究

为了充分利用酒糟资源，减轻酒糟对环境的污染，以优良菌种117号产朊假丝酵母、136号热带假丝酵母、白地霉和扣囊拟内了解霉混合发酵酒糟，生产酒糟单细胞蛋白饲料，结果表明：发酵最适起始pH为5.0左右；适宜的发酵培养基配方为80%酒糟，15%麦麸左右%玉米粉，1%豆饼，1%尿素，0.05%磷酸二氢钾，发酵前用适量糖化酶预处理酒糟能明显提高酒糟蛋白质含量，通过扩大发酵试验，100g酒糟中粗蛋白含量由18.1g增加到28.6g，人和成年哺乳动物必需氨基酸及钙、磷含量显著增加，粗纤维和单宁含量明显减少。酒糟单细胞蛋白饲料可作为配合饲料的新型优质蛋白源，采用分割留种培养能生产高质量、低成本的酒糟单细胞蛋白饲料。     

CALIS成员馆中文图书联机编目程序的质量控制

文章认为CALIS联机合作编目中心对书目数据实行质量控制固然重要，但各成员馆对本馆联机编目的程序进行有效的控制，对于减少或杜绝书目数据整体质量问题更具有积极的意义，据此，阐述了如何对联机编目的各个程序实行有效控制。     

二氧化氯对机械损伤苹果的防腐保鲜效果

【目的】研究在冷藏条件下二氧化氯(ClO2)处理对受损伤苹果果实的防腐保鲜效果。【方法】以采后受损伤的"红富士"苹果为试材,对果实进行压伤、果柄戳伤模拟处理,对压伤果和果柄戳伤果均用50 mg/L ClO2溶液浸泡20 min(分别记为处理A和处理B),以用蒸馏水浸泡20 min的健全果、压伤果和果柄戳伤果作为对照(分别记为CK、CKA、CKB),比较处理和对照果的呼吸速率、乙烯释放速率、失重率和腐烂指数。【结果】CKA和CKB的呼吸速率、乙烯释放速率、失重率和腐烂指数均高于CK,但处理A、处理B果实的上述指标均低于CKA和CKB,其中处理A和处理B果实只有腐烂指数低于CK,其他3个指标均高于CK。【结论】ClO2处理对机械损伤果具有明显的防腐保鲜效果,可有效延长贮藏期。     

The effect and mechanism of H. polygyrus infection in T-cell-mediated inflammatory bowel disease in mice

AIM: To investigate the effect and mechanism of Heligmosomoides polygyrus (H. polygyrus) infection in mouse inflammatory bowel disease (IBD) mediated by CD4⁺ helper T-cells. METHODS: Ovalbumin (OVA) -specific CD4⁺ helper T-cells were transferred into SCID (severe combined immunodeficiency) mice to establish an IBD model. The IBD mice were infected by H. polygyrus and sacrificed 14 days later. The histological changes of the colon were observed, and the expression of interferon-gamma (IFN-γ) and interleukin-4 (IL-4) in mesenteric lymph nodes was detected by ELISA and flow cytometry. Additionally, IL-4 monoclonal antibody was intraperitoneally injected into the H. polygyrus-infected IBD mice to block the secretion of IL-4. The IL-4-blocking IBD mice were sacrificed 9 days later and the above indexes were also determined.RESULTS: Compared with the non-infection group, the H. polygyrus-infected IBD mice had more severe colonic lesions, higher level of IL-4 and lower level of IFN-γ in mesenteric lymph nodes (all P<0.05). Compared with the non-blocking group, the H. polygyrus-infected IBD mice with IL-4 blockage had less colonic lesions, lower IL-4 level and higher IFN-γ level (all P<0.05).CONCLUSION: H. polygyrus infection in CD4⁺ T-cell-mediated IBD model promotes inflammation in the early stage probably by inducing the secretion of Th2 cytokine and inhibiting the secretion of Th1 cytokine. The finding suggests that using worms for treatment of IBD needs to be cautious.