The influence of protein level in the diet on nitrogen content in the total, and in the gain of body mass of growing male rats

The experiment was conducted on 364 male Wistar rats of which 74 were included into the "zero" group and the remaining 290 were divided into 5 experimental groups, corresponding to the dietary protein levels of 4, 5, 10, 15 and 20%. The protein source in the diet was skim milk powder. The animals were fed individually and ad libitum. The experiment was performed between the 21 and 66 days of life of the rats and during this period, the animals in each group were successively killed to determine the nitrogen content in the body. Between the N content in the body and the body mass of rats from "zero" group, the relationship N0/W0 was determined. In each experimental group, two relationships were determined: --final nitrogen against final body mass--Nf/Wf --gain of nitrogen against gain of body mass--delta N/delta W. The derivatives of both functions were used for calculation of the daily gains of nitrogen (delta Nd) 1. According to the relationship Nf/Wf there was a similar significant increase of nitrogen content in the body in all groups with increasing the body mass, according to bc = 1.0413. The percentage of nitrogen in the body increased significantly only at dietary protein levels over 10%. 2. The rate of nitrogen gain in the gain of body mass according to delta N magnitude of was relatively constant in all groups (bc = 1). Thus, N-% in delta W was also relatively constant. N-% in the body gain increased significantly only at dietary protein levels over 10%. 3. Differences in the gains of nitrogen, as calculated by the derivative dNf/dWf and by d delta N/d delta W, showed in the first line in low-protein groups (on 4 and 5% protein) due to the error of estimation of the initial-N according to the equation N0 magnitude of W0. The mean relative error of estimation the initial-N was +/- 3% but in groups on 4 and 5% protein it charged the gain of N with 9-12% in spite of excluding from the relationship delta N magnitude of delta W rats with delta W less than 10 g. 4. Only when delta N greater than or equal to 1.3 g and delta W greater than or equal to 43, the error of estimation of delta N, did not exceed 3%.(ABSTRACT TRUNCATED AT 400 WORDS)     

Detection of Salmonella-containing food using the 1-2 test

133 samples of food were investigated for comparison of a recent commercially available 1-2 test (Biocontrol) with a cultural standard method (non-selective pre-enrichment in buffered peptone water, selective enrichment in Rappaport-Vassiliadis-medium and selenite brilliant-green mannitol enrichment broth, inoculation on two selective agars) for presence of Salmonella. The 1-2 test showed in positive results an accuracy ("sensitivity") of 94.7% and in negative results an assurance ("specificity") of 97.9% and is therefore considered suitable for detection of Salmonella contaminated food.     

Clinical diagnostic keys and special manifestations in equine leukosis

The literature contains about 500 cases of equine leucosis, though the reports are deposited in a great number of journals and vary considerably concerning particular topics. During the last years there has been a remarkable increase of publications about this syndrome in the equine. The clinical leucosis key recommended by us has been confirmed in principle considering the latest literature. In about 70 individual symptoms which can be clinically observed in equine with leucosis 11 can be considered as main symptoms because of their frequency; they are again classified in primary (lymph node tumours including splenomegaly--loss of condition, weakness--cachexia, weight loss, periphery oedema), secondary (anorexia, inappetence--fever--paleness of mucous membrane--anaemia--tachycardia) and accessory (incoordination--tachypnoea, dyspnoea--apathy, lethargy) main symptoms. Furthermore in future it will be necessary to take into more consideration the symptoms "recurrent colic" and "hydrothorax" within differential diagnosis. The main symptom "incoordination" (ataxia, asynergy, paresis, paralysis) is used by us more precisely only in case of impairment of nervous system by neoplastic infiltrations and does not signify as possible symptoms of general physical weakness, for example faltering, staggering, tumbling or lameness. The morphological classification follows further on our previous recommendation. There exist generalized forms with tumour infiltrations in abdominal and in thoracic cavity as well as especially in peripheral lymph nodes. On the other hand there are characteristic manifestations in certain regions of the body, which establish distinctly the clinical symptomatology. They are marked as regional multicentric forms with the main localizations "mediastinal", "splenic", "mesenteric" or "intestinal".(ABSTRACT TRUNCATED AT 250 WORDS)     

Age and exercise effects on mitochondrial density and capillary fibre ratio in bird leg muscle

1. Muscle fibre composition, capillary-fibre ratio and mitochondrial density of the Iliotibialis lateralis muscle were monitored during growth from the age of 8 weeks to sexual maturity (24 weeks) in sedentary birds and in birds subjected to regular treadmill exercise. 2. The Iliotibialis lateralis muscle consists almost exclusively of type IIA and IIB fibres with a small percentage of "unclassified" fibres. Over the 16-week period of growth there was no change in fibre composition but a marked fibre hypertrophy linked with a fall in the capillary-fibre ratio. Mitochondrial volume density increased with age but this was not significant. 3. Exercise significantly increased the IIA:IIB fibres ratio from 0.77 to 1.1 but had no significant effect on any other measured characteristics. 4. It is concluded that exercise at the intensity and duration employed (10-15 min daily, ca. 70-80% maximum oxygen consumption) has little effect on the growth and development of avian skeletal muscle although there was evidence of an increased oxidative capacity of the muscle.     

Diagnosis of equine stifle joint disorders: three cases

The clinical, radiographic, arthrographic, arthroscopic, and pathological findings of three horses with femorotibial joint injuries are presented. Overall diagnostic accuracy is improved when clinical signs, arthrography, and arthroscopy are combined. Treatment of these injuries remains limited. One horse was euthanized, and two were treated by stall rest after diagnosis. Of the two surviving horses, one became a successful breeding animal, and the other was unable to perform as a pleasure horse.