低海拔异地育肥牦牛与本地杂交肉牛(秦川×西门塔尔)在不同非蛋白氮水平饲粮条件下血液生理生化指标及生长性能的差异

为探究异地育肥牦牛模式,本试验对比了低海拔异地育肥耗牛与本地杂交肉牛(秦川×西门塔尔)在同等条件下血液生理生化指标和生长性能的差异。将体重相近的8头1岁本地杂交肉牛(秦川×西门塔尔)和8头4岁青海牦牛各分为2组,每组4头,同一品种的2组试验牛分别饲喂低非蛋白氮饲粮[LNPN,饲粮中缓释尿素添加量为1.0%(干物质基础)]和高非蛋白氮饲粮[HNPN,饲粮中缓释尿素添加量为1.5%(干物质基础)]。预试期10 d,正试期50 d。结果发现:牦牛与肉牛均在LNPN条件下表现出最高的平均日采食量和最小的料重比。饲粮非蛋白氮水平未对牦牛与肉牛的血常规指标造成显著影响(P0.05),但是牦牛在各饲粮条件下中性粒细胞数目(Gran#)、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、红细胞分布宽度标准差(RDW-SD)、平均血小板体积(MPV)和血小板分布宽度(PDW)显著高于肉牛(P0.05),而平均红细胞血红蛋白浓度(M CHC)和血小板数目(PLT)显著低于肉牛(P0.05)。饲粮非蛋白氮水平未对肉牛和牦牛各项血清生化指标产生显著影响(P0.05)。牦牛在各饲粮条件下血清总蛋白(TP)、尿素氮(UN)、肌酐(GREA)、免疫球蛋白A(Ig A)、免疫球蛋白M(Ig M)和免疫球蛋白G(Ig G)水平均显著高于肉牛(P0.05)。牦牛在HNPN条件下的血清白蛋白(ALB)水平显著低于LNPN条件下(P0.05)。本试验结果表明,在肉牛和牦牛育肥饲粮中添加1.0%的缓释尿素替代饲粮蛋白质是可行的;低海拔异地育肥牦牛不会对牦牛健康产生不利影响。     

Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent

In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.     

HPLC法测定咪松酚乳膏中硝酸咪康唑、醋酸地塞米松及丹皮酚的含量

采用超声波及冰浴法对咪松酚乳膏进行前处理，以反相高效液相色谱法同时测定了咪松酚乳膏中硝酸咪康唑、醋酸地塞米松和丹皮酚的含量.硝酸咪康唑线性范围为40.5~648.0 μg/mL，r=0.999 1;醋酸地塞米松线性范围为3.05~48.8 μg/mL, r=0.999 8；丹皮酚线性范围为20.5~328.0 μg/mL, r=0.999 4；硝酸咪康唑、醋酸地塞米松及丹皮酚的平均回收率分别为：100.44±1.25(%)， 98.46±1.04(%) 和98.24±1.29(%).     

土耳其斯坦裂体吸虫23kDa蛋白基因的序列分析及抗原表位预测

为研究土耳其斯坦裂体吸虫23 kDa蛋白的生物学特性,以土耳其斯坦裂体吸虫cDNA为模版,利用PCR对23 kDa基因进行扩增,并将其克隆到T-easy载体后进行序列测定。利用生物信息学对其结构、抗原指数进行分析,并对其抗原表位进行预测。序列分析结果表明,该虫体的23 kDa基因长度为657 bp,A+T含量为57.38%,与曼氏血吸虫、埃及血吸虫和日本血吸虫的23 kDa基因的相似性分别为85.24%、83.71%和81.89%。蛋白二级结构分析表明,23 kDa蛋白经过4次跨膜,主要由6个α-螺旋、3个β-折叠、7个β转角和若干个无规则卷曲构成。抗原表位预测结果表明,该蛋白有3个B细胞抗原表位。综合分析土耳其斯坦裂体吸虫23 kDa蛋白是一种较好的抗原分子,是土耳其斯坦裂体吸虫疫苗的重要候选分子。     

厚皮甜瓜新品种瓜州王子6号

<正>瓜州王子6号全生育期为78~90 d(天),中早熟。果实椭圆形,表皮坚硬,耐贮运性好,果肉浅绿色,肉质细腻稍脆,中心可溶性固形物含量14.5%~18.0%,有清香味,平均单瓜质量2.5 kg,每667 m2产量3 270 kg左右。甘肃省酒泉市甜瓜种植历史已逾2 000 a (年),生产的甜瓜甜如蜜、脆如梨,风味独特。酒泉市农     

加工番茄酱厂生产线自动除杂装置的设计

研制了用于番茄酱厂生产线中自动清理杂草、番茄皮等杂质的自动除杂装置。该装置利用光电开关识别杂质收集箱内杂质的容量,采用可靠性和抗干扰性强的PLC控制的气压缸执行,从而完成整个杂质的分选、清理过程。     

产甲壳素酶菌株的筛选与诱变

以胶体甲壳素作为惟一碳源,从自然界微生物中筛选并诱导到一株产甲壳素酶活性较高的菌株,经16S r DNA鉴定为Aeromonas sp.。并以该菌株作为出发菌株,经过一次紫外诱变,酶活是初筛菌株的1.48倍。再经过二次紫外诱变或者紫外-Li Cl复合诱变,酶活分别是初筛菌株的3.16和3.87倍,最高酶活达到3.48 U/m L。最后通过连续传代培养,确定了菌株HMX-16经过紫外和复合诱变后突变性状稳定性良好。     

响应面优化丁烷-亚临界法制取桐油技术的研究

桐油是一种重要用途的干性油脂,不同工艺会对油品质量产生重要影响。为了克服传统工艺在制油中的缺陷,本研究采用丁烷-亚临界法工艺进行桐油的制备,并基于Design-Expert软件进行响应面优化实验,获得最佳工艺参数:萃取温度40.64℃,萃取时间45.38 min,原料颗粒度39.71目,在此条件下桐油得油率达到66.12%。采用气质联用仪分析了桐油脂肪酸组成为α-桐酸74.93%,亚油酸8.62%,油酸7.12%,棕榈酸2.72%,硬脂酸3.85%,通过分析所得油样的5项指标,可知该工艺所得油品满足国标要求。     

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.