大豆萌发过程中谷氨酸脱羧酶活性变化研究

研究了大豆在浸泡、萌发两个过程中谷氨酸脱羧酶(GAD)的活力变化。通过测定大豆中γ-氨基丁酸(GABA)的含量变化,间接测定大豆中GAD的活性。结果表明,大豆在25℃,浸泡10h,大豆中的GAD活性达到浸泡中最高水平,酶活性为2.45U。浸泡后的大豆在30℃,萌发40h时,酶活性达到萌发中最高水平,为1.28U。大豆在浸泡和萌发两个状态下,GAD活性较储藏期都有很大提高,且浸泡时酶活总体水平高于萌发期酶活总体水平,其中大豆GAD在25℃,浸泡10h时活性最高,为2.45U。     

报废汽车回收拆解的绿色无害化管理

对我国报废汽车回收拆解过程中的生产者责任、回收拆解企业要求和零部件再制造进行了分析讨论,在此基础上提出了报废汽车回收拆解过程中的绿色无害化管理的改进意见和建议.     

Mcl-1 involves in G2/M arrest of HL-60 cells induced by diallyl disulfide

AIM: To investigate the role of Mcl-1 in the G₂/M arrest induced by diallyl disulfide (DADS) in leukemic HL-60 cells.METHODS: The inhibitory effect of DADS on human leukemic HL-60 cells was detected by CCK-8 method in vitro. Flow cytometry analysis was employed to observe the cycle arrest in HL-60 cells and the effect of DADS-induced G₂/M arrest on HL-60 cells with Mcl-1 gene knockdown by RNAi silencing. The expression of Mcl-1, PCNA and CDK1 in HL-60 cells treated with DADS was determined by Western blotting. The binding of Mcl-1 with PCNA and CDK1 was detected by coimmuno-precipitation. RESULTS: HL-60 cells were treated with DADS at concentration of 15, 30, 60, 120 or 240 μmol/L for 48 h. The inhibition rates of HL-60 cell proliferation were 31.15%, 55.88%, 66.14%, 75.29% and 80.35%, respectively, and gradually enhanced with the increase in the concentration of DADS (P<0.05). Flow cytometry analysis revealed that the proliferation of HL-60 cells was blocked by DADS in the G₂/M phase. Treatment with DADS for 24 h and 48 h at concentrations of 60 μmol/L and 120 μmol/L, the percentage of G₂/M phase cells increased as compared to the untreated cells (P<0.05). DADS induced arrest of HL-60 cells in G₂/M phase in a time- and dose- dependent manner (P<0.05). The results of Western blot analysis indicated that Mcl-1, PCNA and CDK1 in HL-60 cells were significantly reduced after treated with DADS (P<0.05). HL-60 cell cycle progression delayed by silencing Mcl-1 gene with siRNA technique, suggesting that silence of Mcl-1 gene led to G₂/M arrest. Compared to the cells treated with DADS only, the percentage of G₂/M cells raised in the cells with Mcl-1 gene silencing and treated with DADS (P<0.05), indicating that Mcl-1 gene silencing enhanced the effect of DADS-induced G₂/M arrest in HL-60 cells. The binding of Mcl-1 with PCNA and CDK1 was detected by coimmuno-precipitation and the formation of heterodimers was observed, which was decreased after treated with DADS for 4 h.CONCLUSION: DADS inhibits the proliferation of HL-60 cells and induces its G₂/M phase arrest. The decreased expression of PCNA is related to inhibiting the proliferation of leukemic cells. Knockdown of Mcl-1 gene enhances the effect of DADS-induced G₂/M arrest.     

利用模糊数学方法预测秋白菜适宜收获期

依据秋白菜受冻的日最低气温指标,利用本溪市1957～2008年逐日最低气温资料,应用模糊数学隶属度函数预测日最低≤-7℃的初日趋势,建立多元回归模型预测日最低气温≤-7℃初日,为指导秋白菜适时收获、合理安排上期提供科学的气象依据。     

联合运输机设计忽视的因素

联合运输机组的滚筒出河机在设计，试验研究过程中都忽视了原木重心与原木形心位置不一致的因素。它促使较在径级原木在滚筒出河机上打横，造成停机。     

宜兴市引种美国栎属种源和杂交松试验初报

于 1999年 3月在宜兴市林场建立美国栎属 (包括柳叶栎 5个种源、红栎 2个种源 ) ,杂交松(杂种F1代 ,父本、母本 )以及对照树种青冈、麻栎、小叶栎和马尾松等共 14个处理的引种试验林。通过 1a观测 ,其生长和保存率存在显著变异。 (1)美国栎属生长变异极显著 ,树种间比较是柳叶栎 >红栎 >青冈。在柳叶栎中以 9818号最大 ,982 2号最小。红栎种源间无差异。造林成活率由于是采用容器苗造林 ,达 95 0 0 %～ 10 0 0 0 % ,种间和种源间变异不显著。但保存率变异显著 ,以柳叶栎 9816号和青冈最高 ,为 98 0 0 %～ 90 0 0 % ,红栎 982 1号和柳叶栎 982 3号最低 ,为 6 5 70 %～6 5 80 % ,比造林成活率有很大降低。 (2 )杂交松F1代最大 ,父本次之 ,母本最小。造林成活率和保存率差异均不显著 ,一直保持在平均 99 0 0 %     

新疆农村土地市场建设的目标模式

农村土地市场是土地市场不可缺少和不可分割的部分，加快农村土地市场的培育已成为一项十分紧迫的任务，本文阐述了构建新疆农村土地市场的指导思想，基本原则和与目标、步骤，提出了农用地使用权市场目标模式在农用地市场目标模式。     

冷温血停搏液心肌保护效应血清CK—MB,CTn—T的变化研究

为探讨血停搏液不同灌注对心肌保护效应，２８只低温体外循环犬，按停搏液灌注方式随机分为四组：Ａ组为冷晶组，Ｂ组为冷血组，Ｃ组为温血组，Ｄ组为冷温血组。     

柴达木盆地弃耕地盐渍状况的主分量分析

采用主分量分析（PCA）方法对柴达木盆地弃耕地盐渍化状况进行了研究，结果表明，该地属于中重度氯化物盐渍地、耕作层含盐量在0．5％－4．0％之间，盐分表聚强烈，基本无自然淋溶过程。耕作层第一、二主分量显著，累计贡献率高达95．0％左右，反映了该区土壤含盐量及盐离子分布规律，认为全盐量、Cl^-,Mg^2＋是该弃耕地盐渍环境的特征因子。