泵的虚拟绿色制造技术(VGMT)研究

介绍了虚拟绿色制造技术的概念以及该技术在泵制造业中的具体特点；提出了泵的虚拟绿色制造技术的研究内容；论述了泵的虚拟绿色制造技术研究的主要环节，即过流部件的设计(CAD)、分析(CFD)和制造(CAM)一体化的研究。     

黔西煤矿区周边土壤重金属形态特征、污染评价及富集植物筛选

[目的]研究黔西某煤矿区周边土壤重金属污染情况、重金属形态潜在风险及其周边重金属富集植物,为当地的重金属污染防治提供科学依据。[方法]采用潜在生态风险评价及模糊数学法的两种评价方法(单因素决定模型和加权平均模型)对煤矿区及非煤矿区土壤进行重金属生态风险评价,对影响土壤肥力的土壤理化指标进行检测,利用风险评估编码法对重金属形态进行分析,并采用生物富集系数法对煤矿区周边富集重金属植物进行筛选。[结果]煤矿区Hg,Cd,As,Zn,Cr及Ni平均值含量分别是背景值的2.47,3.65,2.00,1.23,1.74,1.69倍。煤矿区潜在生态危害趋势为:CdHgAsNiCrPbZn。模糊数学法单因素决定模型评价显示,非煤矿区污染大于煤矿区,加权平均模型则反之。煤矿区Cd,Cr,Cu,Mn,Ni,Pb及Zn潜在风险指数分别为69.17%,7.97%,8.24%,40.10%,45.29%,53.70%及29.90%。蜈蚣草对As富集系数大于1.00,火棘、构树、盐肤木、马桑、凤尾蕨及金丝梅等对Cd富集系数大于1.00,马桑及白蒿对Pb富集系数大于1.00。[结论]煤矿区存在重金属污染,以Cd,As,Hg较为严重。煤矿区周边土壤中重金属对环境构成的潜在风险顺序为:CdPbNiMnZnCuCr。对当地而言,蜈蚣草可作为煤矿区周边修复As污染的先行植物,凤尾蕨可作为修复Cd污染的先行植物,马桑可作为修复Pb污染的先行植物。     

miR-221 changes resistance of lung cancer cells to gefitinib by regulating PTEN

AIM: To explore the effect of microRNA-221 (miR-221) on resistance of lung cancer cells to gefitinib, and to investigate its related mechanism. METHODS: RT-qPCR was used to detect the levels of miR-221 expression between gefitinib-sensitive cell line PC9 and gefitinib-resistant cell line PC9/GR. The PC9/GR cells were transfected with miR-221 inhibitor by Lipofectamine 2000. The drug sensitivity of these cells to gefitinib was determined by CCK-8 assay. The protein expression level of phosphatase and tensin homologue deleted on chromosome ten (PTEN) was determined by Western blot. The 3'-UTR of PTEN was cloned into luciferase reporter vector and its luciferase activity was detected to verify whether miR-221 targets PTEN. RESULTS: The expression level of miR-221 in the PC9/GR cells was significantly higher than that in the PC9 cells (P<0.05). The protein expression level of PTEN in the PC9/GR cells was lower than that in the PC9 cells (P<0.05). The IC₅₀ of gefitinib was significantly reduced in the PC9/GR cells after transfection with miR-221 inhibitor (P<0.05). The protein expression level of PTEN in the cells transfected with miR-221 inhibitor was increased as compared with control group and blank group (P<0.05). Inhibition of miR-221 expression enhanced the enzymatic activity of luciferase reporter vector of PTEN. CONCLUSION: miR-221 enhances the resistance of lung cancer cells to gefitinib by down-regulating the protein expression of PTEN.     

江西省森林资源现状及新世纪森林经营对策

以第五次全国森林资源连续清查数据为依据，对江西省森林资源现状进行简要分析。通过现状分析，从提高森林质量及林地生产力、调整商品林内部结构、保护和合理开发森林旅游资源、加强森林资源的价值的价值算研究等方面提出森林经营对策。     

美国山核桃在云南的引种研究进展及发展前景

总结了云南53a来对美国山核桃引种、选种、生物学特性、生态适应性、苗木繁殖、丰产栽培、虫害防治的研究进展和示范推广情况。认为美国山核桃市场潜力大，在云南栽培效益高、适栽区域广、符合该省经济林树种结构调整需要，将来定会得到大力推广发展。     

甬优1540工厂化基质育秧高产栽培技术规程

工厂化基质育秧技术是一项具有保护环境、资源再利用特点的轻简、高产、高效稻作技术。本研究采用大田试验示范、田间定点观察与室内测定相结合的方法,探明了甬优1540在太仓市工厂化基质育秧条件下的生长特性,并制定了相应的高产栽培技术规程。     

城镇水资源环境研究——以水府庙示范区为例

本文针对水府庙水库水环境现状污染的具体情况,研究并提出相应的防治对策,对水府庙水资源的利用、开发和保护,有着重要的现实意义.     

The toxicological effect of dietary excess of saccharicterpenin,the extract of camellia seed meal,in piglets

Recently, saccharicterpenin extracted from Camellia oleifera seed meal has become a widely used feed supplement in animal husbandry. In order to assess its safety, this study was designed to investigate the toxicity and histopathological effects of saccharicterpenin on piglets. One hundred-fifty weaned pigs((Yorkshire×Landrace)×Duroc), 75 males and 75 females with body weight(BW) of(7.35±0.29) kg, were randomly allotted to groups receiving diets supplemented with 0, 500, 1 000, 2 500 or 5 000 mg kg~(–1) saccharicterpenin for 70 d. The diet with 500 mg kg~(–1) saccharicterpenin supplementation improved liver glutathione peroxidase(GSH-Px) activity, and the diet with 1 000 mg kg~(–1) saccharicterpenin supplementation improved liver glutathione S-transferase(GSH-S) activity in piglets on d 70(P0.05). At 2 500 mg kg~(–1), saccharicterpenin in the diets reduced average daily feed intake(ADFI) and average daily gain(ADG) of piglets from d 1 to 35, damaged the cardiac tissue and liver on d 35, and decreased white blood cell counts(WBC), activities of catalase(CAT) and GSH-Px, and concentrations of glucose(GLU) and urea nitrogen(BUN) in the blood of piglets on d 70(P0.05). In addition, diets with 5 000 mg kg~(–1) saccharicterpenin supplementation reduced ADFI, ADG and increased diarrhea rates of piglets from d 36 to 70, and decreased hemoglobin(HGB) concentration and activity of CAT in the blood of piglets on d 70(P0.05). Moreover, at a rate of 5 000 mg kg~(–1), saccharicterpenin supplementation increased pancreas index on d 35 and hepatic index on d 70, and damaged cardiac tissue, liver and spleen during the whole experimental period(P0.05). These results suggested that dietary 500 mg kg~(–1) saccharicterpenin supplementation had beneficial effects on piglets, but excessive supplementation(2 500 or 5 000 mg kg~(–1)) of saccharicterpenin in the diets could lead to growth retardation, hematological abnormalities and organ injuries.     

云南松幼林生长对间伐和修枝的响应

为了解间伐和修枝抚育经营对云南松幼林生长的影响,以昆明市宜良县禄丰林场的尖山林区12 a生1 m×2 m×5 m的非均匀密度控制造林的云南松人工林为研究对象,采用L4(23)正交试验设计开展试验研究,并于抚育前和抚育后1a和2a时进行逐株对应测定和分析.结果表明,抚育后2a时,处理组合的平均胸径、树高和材积增长率分别为...     

配施黄腐酸钾对甘蔗生长和产量的影响

[目的]了解配施黄腐酸钾对甘蔗生产的提质增效作用,筛选适宜蔗区的黄腐酸钾田间用量。[方法]分别在耿马县、双江县和景谷县等3个云南蔗区主产县进行柳城05-136的大田试验。以当地习惯施肥为对照,比较了4种黄腐酸钾配施用量对柳城05-136生长、产量及糖分的影响。[结果]配施黄腐酸钾能有效提高甘蔗株高、有效茎和产量,对提高甘蔗糖分有一定的促进作用。双江县试验点采用当地习惯施肥配施600 kg·hm^-2黄腐酸钾处理(CF₁)较当地习惯施肥增产29.72%;耿马县和景谷县试验点采用87.5%当地习惯施肥配施600 kg·hm^-2黄腐酸钾处理(CF₃)较当地习惯施肥分别增产15.66%和24.61%。[结论]配施黄腐酸钾对甘蔗产量有明显的促进作用,3个试验点均以配施600 kg·hm^-2黄腐酸钾效果最佳。建议在云南蔗区进行黄腐酸钾配施。