Effects of platelet-rich plasma on chondrocyte apoptosis and NLRP3/IL-1β signaling pathway in rabbits with osteoarthritis

AIM To explore the effect of platelet-rich plasma (PRP) on rabbit osteoarthritis and its possible mechanism. METHODS The rabbits with knee osteoarthritis were prepared and then divided into model group, sodium hyaluronate (SH) group and PRP group, and another sham operation group was set up, with 6 rabbits in each group. The gross morphological changes of rabbit cartilage were observed. HE staining was used to evaluate the pathomorphological changes of the cartilage. TUNEL staining was used to detect the apoptosis of chondrocytes. The expression of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)/interleukin-1β （IL-1β） signaling pathway-related molecules was observed by immunohistochemical staining, and the protein levels of caspase-3, Bcl-2 and Bax were determined by Western blot. Chondrocytes were isolated and processed according to grouping, and the NLRP3 and IL-1β levels of the cells were measured by ELISA. RESULTS Compared with sham operation group, Pelletier score, Mankin score, chondrocyte apoptotic rate, the positive protein expression rates of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax in model group were increased significantly (P<0.05), while the protein expression of Bcl-2 was decreased significantly (P<0.05). Compared with model group, Pelletier score, Mankin score, the apoptotic rate of chondrocytes, the positive protein expression rates of NLRP3, ASC, caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax in SH group and PRP group were decreased significantly (P<0.05), while the protein expression of Bcl-2 was increased significantly (P<0.05). In PRP group, Pelletier score, Mankin score, the apoptotic rate of chondrocytes, the positive protein expression rates of NLRP3, ASC, caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax were lower than those in SH group, while the protein expression of Bcl-2 was higher than that in SH group (P<0.05). Compared with control group, the expression of NL?RP3 and IL-1β in MCC950 (NLRP3 ihibitor) group were significantly reduced (P<0.05), the expression of NLRP3 in eucalyptol (IL-1β inhibitor) group was not significantly changed (P>0.05), and the expression of IL-1β was significantly reduced (P<0.05). CONCLUSION Platelet-rich plasma promotes the repair of cartilage in osteoarthritis rabbits, which has better effect than SH. The mechanism may be related to the inhibition of NLRP3/IL-1β pathway and the reduction of chondrocyte apoptosis.     

甜瓜嫩果皮颜色遗传规律研究

为探究甜瓜嫩果皮颜色的遗传规律,以甜瓜嫩果皮颜色为深绿色的S26和青色的S28为亲本材料,通过构建BC1群体,利用卡方测验,分析回交群体BC1的深绿色嫩果皮和青色嫩果皮的分离比例,开展甜瓜嫩果皮的遗传规律分析,以对嫩果皮颜色基因进行初步定位。结果表明：BC1群体表型表现为深绿色和青色2种,且群体比例为1∶1,从而确定颜色性状为单基因控制且深绿色为显性性状。通过分离群体分组分析法（BSA）和混池测序结果可以看出,控制甜瓜嫩果皮颜色的基因位于chr04的端部位置,这为后续基因的精细定位以及克隆提供了研究基础。     

小径木梯形板锯刨机结构设计与研究

针对目前市面上加工小径木的机器加工精度低、效率不高、出材率低等问题,分析提高小径木梯形板出材率、加工效率及加工精度的设计方法,通过新的设计方法制定了新的加工工艺。结合工艺分析设计了小径木梯形板锯刨机的总体结构,可以对小径木梯形板进行锯切、粗刨、精刨加工。在对其核心组件锯切组件与刨削组件的加工原理进行分析的基础上,设计了锯切组件结构与刨削组件结构。通过导入锯切主轴SolidWorks的三维模型,利用ANSYS对其锯切主轴进行静力学分析,得到锯切主轴的应力、应变和变形云图,其中最大应力值为7.435 MPa、最大应变值为0.042 316 mm/m、最大变形量为0.002 178 8 mm,其强度、刚度及变形量均在许用范围内,仿真验证了锯切主轴的设计的合理性。     

高淀粉甘薯品种栽培模式及影响效应初探

为了探索高淀粉甘薯[Ipomoea batatas (Linn.) Lamarck]品种的高产栽培模式,选取肥料种类、甘薯品种、种植密度、覆膜类型4个因素,采用随机区组正交试验设计,比较不同因素对茎叶产量、鲜薯产量、干物质率、T/R(蔓、薯鲜重比值)的影响。结果表明,组合A_3B_1C_3D_2,即施用复合肥、甘薯品种选用商薯19、覆盖黑膜、种植密度为59 895株/hm~2时的鲜薯产量最高。肥料种类以复合肥的茎叶产量、干物率及鲜薯产量最高;甘薯品种对茎叶产量及T/R影响效应最显著;覆膜能提高茎叶产量、干物率和鲜薯产量;种植密度对干物率影响最明显。     

基质深度及基质袋摆放方式对春季袋培番茄产量、品质和养分吸收的影响

为探究不同栽培深度基质和基质袋摆放对春季袋培番茄产量、品质、养分吸收和基质养分利用率的影响,以‘巴宝丽’番茄为试材,设置不同基质深度(7.5、10.5和13.5cm)及基质袋不同摆放方式(地面摆放和地面下沉20cm),共6个处理,测定了番茄的生长发育、产量、品质和养分吸收等指标。结果表明:栽培基质深度对产量、果实品质均有显著影响;基质袋摆放方式对产量影响不显著,但显著影响果实可溶性蛋白、番茄红素、硝酸盐和有机酸含量;基质深度和基质袋摆放方式对果实硝酸盐含量的影响有显著的交互作用。随着栽培基质深度的增加,产量显著提高,品质明显改善;基质深度13.5cm时,番茄单株产量最高,达3.83kg/株。基质袋摆放方式和基质深度对番茄开花期、初果期及盛果期P和K的累积吸收影响显著,基质深度对番茄开花期、初果期和盛果期N的吸收也有显著影响,基质深度和基质袋摆放方式对开花期和初果期番茄植株N、P和K的累积吸收存在显著的交互作用。基质内N、P和K养分利用率均是基质深度为13.5cm的处理最高,且不同基质袋摆放方式对基质养分利用率无显著影响。综上,为实现省工省力且高产优质载培,在实际生产中推荐将春季栽培番茄的基质深度设置为13.5cm(即基质供应量为9L/株)且地面摆放。     

积造农家肥改良盐碱地是实现粮食增产的根本途径

吉林省双辽市柳条乡吉兴村多年积造农家肥改良盐碱地收到较好的效果,玉米产量由过去3 700kg/hm2,增加到7 500 kg/hm2;人均收入由过去的800多元,增加到3000元。