富硒和γ-氨基丁酸萌芽糙薏米培养液组分的工艺优化

为提高糙薏米中γ-氨基丁酸的富集量,采用响应面试验法对糙薏米的培养液组分进行工艺优化。结果表明:培养液中Na2SeO3浓度以10mg/L为宜,其发芽率最高,达(90.33±1.73)%;在Na2SeO3浓度为10mg/L的培养液中辅以11mmol/L CaCl2、87.7μmol/L GA3和31.5mmol/L MSG进行萌芽处理时,萌芽糙薏米中总硒、有机硒和GABA含量分别达0.42mg/100g、0.37mg/100g和116.69mg/100g,是一种具高值化产品开发潜力的保健食品基料。     

不同样品对黄羽种鸡禽白血病病毒净化检测效果的影响

【目的】评估黄羽种鸡禽白血病的净化工作,比较源于同一鸡群不同样品对禽白血病病毒(ALV)检测结果的影响.【方法】采用ALV抗原ELISA方法对广东一黄羽种鸡场采集的2 691枚种蛋进行检测,根据检测结果采集其中70份不同S/P区间所对应的母鸡抗凝血,分离血浆后分别同时接种于CEF和DF-1细胞,用病毒分离方法进行检测,并用PCR方法进行亚群鉴定.【结果和结论】从送检种蛋蛋清共检出ALV p27阳性样品243份,阳性率为9.03%(243/2 691);蛋清不同ELISA S/P区间所对应的病毒分离情况分别为:蛋清ELISA S/P≥2.0的鸡只其血样CEF和DF-1细胞病毒分离率均为100%;1.5≤S/P2.0的鸡只病毒分离率均为88.9%;1.0≤S/P1.5的鸡只病毒分离率均为85.7%;0.2≤S/P1.0的鸡只病毒分离率分别为60%~75%(CEF)和40%~50%(DF-1);0.1≤S/P0.2的鸡只病毒分离率为62.5%(CEF)和50%(DF-1);S/P0.1的鸡只分别为27.2%(CEF)和9.1%(DF-1)的病毒分离率.PCR结果显示,所有7份CEF+DF-1-的CEF培养物中有6份为内源性ALV-E.研究表明,在蛋清ELISA检测时,S/P越高的阳性蛋清样品其对应母鸡越可能是外源性ALV病毒血症阳性;有一定比例S/P较低的阳性蛋清样品是由对应母鸡体内的内源性ALV排毒所致;而净化初期少数蛋清S/P为阴性的蛋清,其对应母鸡仍可能检出外源性ALV,说明在黄羽种鸡外源性ALV净化方案中单独使用1次蛋清ELISA检测可能会给禽白血病的净化检测造成一定的"误诊"和"漏检".     

长丰鲢各部位主要营养成分分析及比较

长丰鲢是人工培育出的鲢鱼新品种。为充分了解长丰鲢的营养特性,更好地利用长丰鲢资源,对北方地区养殖的长丰鲢各部位主要营养成分进行分析,用气相色谱法(GC)测定其不同部位的脂肪酸种类和含量,并将其肌肉营养成分和脂肪酸组成与普通白鲢进行对比。结果表明:长丰鲢各部位间的营养成分存在差异,其中肌肉中的粗脂肪和粗蛋白质量分数较高,分别为3.00%和16.21%;鳍中灰分的质量分数为23.64%,是肌肉中的20倍;内脏中水分和脂肪的质量分数最高,分别为75.68%和7.41%。长丰鲢各部位的不饱和脂肪酸含量较高,鱼肉不饱和脂肪酸中C18∶1n9的质量分数最高,为21.20%。肌肉中ΣEPA+DHA的质量分数为8.48%,鱼头中为7.49%,高于其他部位。与普通白鲢相比,长丰鲢肌肉中的α-亚麻酸的含量高于白鲢,但ΣEPA+DHA低于白鲢,主要营养成分差别不大。     

中国猪肉产业链市场价格传导机制

为了解猪肉产业链各环节价格的传导机制,考察各价格传导方向及传导路径是否畅通,采用中国牧业信息网及商务部2004年1月—2014年3月的月度数据,运用协整检验和向量误差修正模型对猪肉产业链各环节的价格传导机制进行实证分析。中国猪肉产业链各环节市场价格存在长期的均衡关系;误差修正模型中的误差修正的强度为2.3%,对促使市场价格系统均衡影响较小,因此不能完全依靠市场供求系统的自发力量调节猪肉产业链中各种价格的系统均衡;猪肉产业链各环节间价格传导不是完全通畅,有些环节存在1期或2期的传导时滞;猪肉产业链的价格传导表现为下游向上游传导、下游向中游传导以及中游向上游传导,是需求拉动型。应密切关注猪肉市场零售价格,以零售价格为信号,预测产业链各环节价格的近期走势,以利于养殖户和批发商准确把握市场信息,避免价格波动带来的收入损失。     

Study on Chemical Components of Alkaline Nitrobenzene Oxidation from Plantation Woods

This paper deals with the chemical components and the chemical structure of lignin of Chinese fir and Poplar I-214 from plantationforest. The results revealed that the contents ofchemical component in heart wood and sapwood were almost the same except ethanol-benzene extract both in Chinese fir and in Poplar I-214, The contents of ash, holocellulose, α -cellulose, pentosan, ethanol-benzene extract and cold-water extract in Poplar I-214 were higher than that in Chinesefir, The content of lignin in Poplar I-214 was lower than that in Chinese flr. Vanillin, vanillic acid and p-hydroxybenzaldehyde were found in Chinesefir while vanillin and vanillic acid, p-hydroxybenzoic acid, syringaldehyde and syringic acid werefound in Poplar I-214 in investigation of the chemical structure of lignin by using nitrobenzene oxidization method.     

Virtual Sound Intensity Analyzer

The Virtual Sound Intensity Analyzer is a new instrument which has more advantages than the traditional Sound Analyzer. This Analyzer is based on fast Fourie transformation and virtual instrument technique.As an application, combining software and hardware, all kinds of weight noise sound level measurement and real-time noise octave analyzes realized synchronously. The design method has been introduced from three ways, which includes hardware, software and panel. An applied example and error analyze of Virtual Sound Intensity Analyzer are given.This Analyzer is low cost, small volume. and it is also easy to operate. It can be widely used in actual applications.     

Inflammation and coagulation activation are associated with gallstone formation in golden hamsters

AIM: To elucidate the relationship of inflammation, coagulation and cholesterol gallstone formation. METHODS: Hamsters were divided into four groups: control group (feeding normal diet), lithogenic diet (LD) 2 weeks group, LD 6 weeks group and LD+aspirin 6 weeks group. Gallstone incidence, antithrombin antigen (AT-Ⅲ:Ag), antithrombin activity (AT-Ⅲ:Ac), thrombin (F-Ⅱa:Ac), plasminogen activator inhibitor activity (PAI:Ac), plasmin activity (Plm:Ac), D-dimer:Ag and C-reactive protein (CRP) in gallbladder bile were observed as read-out parameters. RESULTS: The incidence of gallstones in control group, LD 2 weeks group, LD 6 weeks group and LD+aspirin group were 0%, 20%, 73% and 25%, respectively. AT-Ⅲ:Ag, F-Ⅱa:Ac, D-dimer and CRP in LD 2 weeks group and LD 6 weeks group were significantly higher than those in the control group (P<0.01). AT-Ⅲ:Ag, PAI:Ac, Plm:Ac, D-dimer and CRP in LD 6 weeks group were significantly higher than those in LD 2 weeks group (P<0.01). AT-Ⅲ:Ag, F-Ⅱa:Ac, PAI:Ac, D-dimer and CRP were strikingly decreased in LD+aspirin group compared with those in LD 6 weeks group (P<0.01). The level of CRP was closely correlated with the concentration of D-dimer (γ=0.752, P<0.01). CONCLUSIONS: Coagulation cascade is activated and crosslinked fibrin forms in gallbladder bile before gallstone formation. “Lithogenic bile” might play a central role in the inflammation of gallbladder mucosa, which activates extrinsic coagulation pathway and leads cholesterol gallstone formation.     

等离子体处理苎麻织物性能研究

本文介绍了等离子体处理苎麻的实验，测试了苎麻织物的纤维在等离子体处理前后的形貌特性和X射线衍射分析；用四被质谱计在线检测了射频放电产生的氧等离子体处理苎麻过程中的主要残气质谱，在氧等离子体处理时真空室的残气中有大量的氢、碳、氧及其化合物产生。测试表明等离子体处理苎麻织物时等离子中的荷能粒子和活性分子对苎麻纤维有较强改性作用，能有效改善苎麻织物性能。     

12月龄生长母水牛绝食代谢的研究

用传统开路式牛用呼吸面具对12月龄母水牛绝食产热(FHP)进行研究。结果表明:①12月龄母水牛FHP为334.03KJ/KgW0.75·d;每天排出内源尿氮(EUN)为31.06g;代谢体重每天排出EUN为0.53g;蛋白分解产热占总产热量为17.99%;EUN与FHP比值为1.60mg/KJ。②12月龄母水牛维持净能需要:NEm=400.84KJ/W0.75d。