赛里木湖饵料生物及渔业现状的研究

赛里木湖为高山冷水型封闭湖,面积453km^2,年平均水温7度,1997年,透明度13m,离子总量2967mg/l,水化学型为SⅡ^Mg,浮游植物平均0．436mg/l,以硅藻和蓝藻为主;浮游动物平均为0．3225mg/l,以西藏拟蚤和镖水蚤为主;初级生产力为2．65Kg鱼／亩．年,自1968年引入新疆土著鱼类以来,至今未形成商业产量,1996年试引虹鳟,生长速度较佳,为开发该湖丰富的冷水资源,建议移植冷水性浮游生物食性的鱼类,改变引种结构,达到商业开发目的。     

The diving behaviour of little penguins in Western Australia predisposes them to risk of injury by watercraft

1. The most western little penguin colony globally, and the most northern in Western Australia (WA) is found on Penguin Island, WA. The penguins use coastal bays that are also used extensively by recreational watercraft. These penguins have been found to either dive predominantly to shallow depths of 1–5 m or to depths >8 m. It is thus hypothesized that (a) both the shallow and deeper diving penguins can potentially be disturbed or injured by these watercraft but that the risk will differ between the two diving strategies, and (b) that risk of injury for both is greater during the summer and autumn, when people are more likely to use watercraft.
2. This was tested by attaching data loggers to little penguins during chick rearing and by investigating necropsy records. Diving activity was studied for the very shallow and relatively deeper diving penguins separately, and we considered the penguins were vulnerable to interactions with watercraft when they were within the top 2 m of the water column or at the surface.
3. Shallow‐diving penguins executed >1,200 dives per day, 64% of dives occurred within the top 2 m, and they were vulnerable for approximately two‐thirds of their time at sea. The deeper diving penguins executed fewer dives. Almost half of dives were to ≥10 m, yet they were vulnerable for almost one‐third of their time at sea. Their post‐dive recovery was also longer. Thus, the risk of interaction from watercraft differs depending on the diving behaviour.
4. This study highlights the potential impact to little penguins throughout Australia and New Zealand.

     

农民专业合作社财务管理工作存在的问题及对策

农民专业合作社作为提高农民组织化程度、维护农民利益、增加农民收入的一种新的组织形式,要想发展壮大,必须重视财务管理。在阐述农民专业合作社财务管理工作重要性的基础上,详细分析当前合作社财务管理工作中存在的主要问题及可能造成的不良后果,并针对这些问题提出切实可行的对策。     

不同浓度NAA/6BA配比对不同品种甘薯茎尖培养特性的影响

为明确不同品种甘薯茎尖培养的最佳NAA/6BA配比,设置0.1 mg/L/2.5 mg/L、0.2 mg/L/2.5 mg/L、0.2 mg/L/1.0 mg/L 3组NAA/6BA浓度配比处理,观测不同浓度激素处理对‘北京553’、‘红香蕉’、‘苏薯8号’、‘烟薯25’、‘安吉芋’、‘济徐23’、‘渝紫7号’、‘商薯19’茎尖培养成苗率、愈伤组织直径、不定根数目、叶片数和植株高度的影响,同时调查不同品种试管苗的移栽成活率。结果表明,‘红香蕉’、‘济徐23’、‘渝紫7号’、‘商薯19’在0.1 mg/L/2.5 mg/L的处理下培养效率最高;‘安吉芋’在0.2 mg/L/2.5 mg/L的处理下培养效率最高;‘苏薯8号’、‘北京553’、‘烟薯25’在0.2 mg/L/1.0 mg/L的处理下培养效率最高。因此,在甘薯茎尖培养过程中,不同甘薯品种适宜的NAA/6BA配比存在显著差异,在实际生产中应根据品种特性来进行调整激素的用量和比例。     

重组球孢白僵菌表达目标产物类枯草杆菌蛋白酶的发酵特性研究

采用单因子筛选和正交试验,研究了碳源、氮源、微量元素及无机盐等营养成分对重组球孢白僵菌(Beauveria bassiana)菌株Bb0062-15-4-CDEP-1产生目标产物类枯草杆菌蛋白酶CDEP-1的影响,并通过摇瓶发酵和30 L发酵罐发酵对其发酵特性进行了研究.结果表明,其优化发酵培养基为2.0%玉米粉,1.0%麦麸,0.25%豆粕粉,0.4%NaNO3,0.1%MgSO4·7H2O.摇瓶发酵结果表明,在对数生长期,随着菌体生物量的增加,CDEP-1产量上升,二者呈一定的平行关系;当菌体生长到稳定期,CDEP-1产量继续上升,并达到产酶高峰;此后,随着菌体生长的衰退,CDEP-1产量出现快速下降趋势.扩大培养时,30 L发酵罐中60 h产酶量最高,比摇瓶培养(168 h)缩短了108 h,单位体积的产酶量比摇瓶培养提高80%.结果对研究和利用该球孢白僵菌重组菌株的代谢产物,提高真菌杀虫剂的杀虫效果奠定了基础.     

Effect of extracellular HSP70/HSP70-PCs on epithelial-mesenchymal transition of human hepatocellular carcinoma HepG2 cells

AIM：To investigate the effect of extracellular heat-shock protein 70 (HSP70)/HSP70-peptide complexes (HSP70-PCs) on epithelial-mesenchymal transition (EMT) of human hepatocellular carcinoma HepG2 cells and its probable mechanism. METHODS：HepG2 cells were divided into 3 groups: control group, HSP70/HSP70-PCs (2 mg/L) group and LY294002+HSP70/HSP70-PCs group. The mRNA and protein expression of epithelial cell surface marker E-cadherin, mesenchymal cell surface marker α-smooth muscle actin (α-SMA), phosphatidylinositol 3-kinase (PI3K) and hypoxia-inducible factor 1α (HIF-1α) was examined by real-time RT-PCR and Western blotting. RESULTS：Extracellular HSP70/HSP70-PCs promoted the initiation of EMT of HepG2 cells. The expression of HIF-1α and PI3K significantly increased in the process of EMT of HepG2 cells. After PI3K was blocked by LY294002, EMT did not occur and HIF-1α was not up-regulated in HepG2 cells. CONCLUSION：Extracellular HSP70/HSP70-PCs may promote EMT of hepatocellular carcinoma cells via PI3K/HIF-1α signaling pathway.     

社会主义初级阶段和阶段性特征是我们谋改革、求发展的立足点

改革开放近30年来,我国取得了举世瞩目的成就,但是人口多、基础差、人民群众尚不富裕、发展不平衡的基本国情没有根本改变,这就要求我们必须清醒地认识我国仍将处于并将长期处于社会主义初级阶段这一基本国情;同时,也要清醒认识我国发展的阶段性特征。深刻认识国情和所处的历史阶段,是正确提出和贯彻党的理论和路线、方针、政策的基本依据,也是推进各项事业发展的重要前提。当前我们贯彻落实科学发展观、深化改革开放的基本立足点就是社会主义初级阶段和阶段性特征。     

5种不同品系相思木材的化学性质 Ⅱ.pH值和酸、碱缓冲容量

对5种相思树人工林木材的pH值和酸、碱缓冲容量的研究结果表明：5种相思树人工林木材之间pH值变异较小，其pH值在4．8～5．9之间；厚荚相思木材心材、边材之间pH值变化较大；木材的酸缓冲容量在2．7～8．35mL范围，碱缓冲容量在21．05～42．00mL范围内变化；5种相思树人工林木材的酸、碱缓冲容量的变化曲线变化趋势大体相同。     

甘蔗间套种/轮种玉米模式调查与分析

甘蔗是我国重要的糖料作物,对我国食糖安全起重要作用。但由于人工成本、地租等生产成本不断升高,导致甘蔗效益逐年减少。因此,如何提高单位面积甘蔗效益是当前甘蔗产业面临的重大问题。采用文献数据、面上调查、数据收集、实地调查和试验结果相结合的调查方法,进行比较分析我国甘蔗间套种玉米历史和现阶段主要模式进行差异分析。宿根蔗间套种玉米主要有每行间种和隔行间种二行两种模式,且宿根蔗间套种模式当前只能靠人工操作,规模化发展难度大;新植蔗间套种玉米的主要方式有等行距每行种植、等行距隔行种植、宽窄行间套种(宽行2.4~3.2米)等模式,但是等行距间套种无法机械化种植难以推广,而宽窄行间套种模式适合机械种植但严重影响单位面积甘蔗苗数;甘蔗轮作玉米能实现机械化种植和管理,但是只能在甘蔗良种繁育基地推广应用。因此,选择适合的间套种品种和合理种植模式还值得进一步研究。     

石蒜C带的研究

对石蒜C带的初步研究表明,其染色体2n=33,由T型染色体组成。带纹不丰富,仅第3号和第5号染色体出现带纹。第3号染色体出现的是着丝点带,为线状;第5号染色体出现的是中间带,带纹出现在长臂上,为斑点状。结果表明,石蒜为同源三倍体。