Knockdown of growth hormone receptor prevents growth hormone induced inflammatory cytokine production in 3T3-L1 adipocytes

AIM: To investigate the effect of growth hormone receptor (GHR) knockdown on nuclear factor-κB (NF-κB) activity and inflammatory cytokine production stimulated by growth hormone (GH) in 3T3-L1 adipocytes. METHODS: The specific siRNA for GHR was transfected into 3T3-L1 adipocytes to silence GHR expressions. The effects of GH on NF-κB activation and inflammatory cytokine production in 3T3-L1 adipocytes transfected with siRNA-GHR or siRNA-control were measured by dual-luciferase system analysis, real-time RT-PCR and ELISA. RESULTS: The protein expression of GHR was diminished after transfection with GHR specific siRNA. Dual-luciferase reporter system analysis revealed that GHR knockdown resulted in attenuation of GH-stimulated NF-κB activation in the 3T3-L1 adipocytes. GHR knockdown ameliorated the GH-induced production of inflammatory cytokines TNF-α, IL-1β, IL-6, MCP-1 and MIP-1α in the 3T3-L1 adipocytes. CONCLUSION: Knockdown of GHR might be efficacious to prevent GH-induced inflammatory responses in the 3T3-L1 adipocytes.     

试析毛泽东在《讲话》中关于文艺知识分子的思想

1942年毛泽东主持召开的延安文艺座谈会,是在抗战后大批文艺知识分子来到延安,给延安文艺界既带来了创作的生力军,同时也产生了一些比较严重的问题的情况下召开的,因此也就有了《在延安文艺座谈会上的讲话》这篇珍贵的讲话稿。在会上,毛泽东关于改造文艺知识分子的一系列观点,抓住了问题的关键,剖析入理,很好地解决了当时延安的文艺知识分子在思想和行为上存在的问题。     

Epidemiological Investigation of Multi-pathogens Co-infection with Post-weaning Multi-systemic Wasting Syndrome

To investigate the epidemiology of multi-pathogens related with post-weaning multi-systemic wasting syndrome (PMWS) in Jiangsu province and surrounding areas,125 healthy samples and 261 diseased samples which obtained in Jiangsu,Anhui and Zhejiang provinces from the January 2014 to May 2015 were screened by PCR for the presence of PCV2,PRRSV,PPV,TTV and P1.These results revealed the following infection rates of PCV2,PRRSV,PPV,TTV1,TTV2 and P1 were 39.38%,21.76%,3.11%,15.80%,16.32% and 10.10%,respectively.In comparison,the co-infection of several viruses occurred in the diseased pigs,and the main co-infections were PCV2+TTV2 (15.32%) and PCV2+PRRSV (11.87%).These observations indicated that the phenomenon of co-infection with multi-pathogens had been widely existing in swine,which increased the difficulty of prevention and controlling of the PMWS.     

实施良好农业规范(GAP)蔬菜生产的病虫害关键点控制

根据中国良好农业规范(China GAP)标准相关控制点的要求,并结合我国蔬菜生产管理的实际情况,对GAP生产管理体系内蔬菜病虫害的关键点及控制技术进行了阐述。     

大学校园流行语与高校校园文化建设

大学校园流行语是一种特定的语言现象。透过大学校园流行语这一语言现象，可从一个侧面了解当代大学生们的思想动态。应重视大学校园流行语，加强教育和引导，营造良好的校园文化氛围，让校园流行语成为校园文化建设的重要组成部分。     

狂犬病病毒免疫组织化学定位方法的建立

应用抗狂犬病病毒的特异性抗体及免疫组织化学方法检测接种了狂犬病病毒SRV-9毒株感染的小鼠脑组织,并对狂犬病病毒抗原进行了组织定位分析。结果表明:在小鼠的小脑分子层蒲肯野氏细胞胞浆出现明显的局灶型阳性颗粒。说明免疫组织化学方法检测狂犬病病毒敏感度高、直观,可作为诊断狂犬病病毒的辅助性方法,对研究狂犬病病毒在脑组织神经细胞及其他组织器官内的分布具有一定的意义。     

Inhibitory effect of geinistein on apoptosis in hUVECs induced by MCP-1

AIM: To study the inhibitory effect of genistein on apoptosis in human umbilical vein endothelial cells (hUVECs) induced by monocyte chemotactic protein-1 (MCP-1). METHODS: The hUVECs were cultured in vitro and identified. Growth-arrested hUVECs were stimulated with genistein at different concentrations (0.1 μmol, 1.0 μmol, 10 μmol, 100 μmol) and co-treated with MCP-1 (10 μg/L). The survival rates of hUVECs were detected by MTT assay. The cell cycle and DNA content were detected by flow cytometry. To explore the possible mechanism of the genistein interventions, the expressions of Bcl-2, Fas and Bax proteins were detected by flow cytometry and Western blotting.RESULTS: Genistein increased the survival rate and the level of Bcl-2, inhibited Fas and Bax, decreased the ratios of apoptosis compared with MCP-1-induced hUVECs apoptotic group in a dose-dependent-manner. CONCLUSION: Genistein inhibits the apoptosis induced by MCP-1 and the inhibitory effect was relative to the dose of genistein. Its mechanism might be involved in the down-regulation of Fas and Bax expressions and the up-regulation of Bcl-2.     

基于价值链理论的精准农业技术构成

提出了运用价值链理论分析农业生产增值环节的基本思路。在分析精准农业技术应用过程中遇到的诸如设备昂贵、技术缺乏、地块偏小、风险偏大和基础设施不健全等矛盾的基础上,构建了以信息网络技术为依托的囊括产前、产中、产后等环节的精准农业价值链模型。在遵循市场需求和实用性原则的前提下,提出了以高级技术引导、特色技术示范和初级技术为推广重点,辅以完善的基础设施技术和先进管理技术的精准农业技术构成体系,以促进农民增收和农业增效为目的,加快我国精准农业技术"本地化"的步伐,使传统农业逐步向现代农业转变。