格点天气预报数据与机理模型耦合的冬小麦干旱预警方法

为了开展大范围的冬小麦干旱预警,以中国北方冬小麦区为实例,构建了土壤水分动态预报模型,结合未来10 d高精度天气要素预报、土壤自动水分观测和冬小麦发育期观测数据,建立了北方冬小麦区干旱预警系统。利用该系统对2018年4—5月进行逐日的冬小麦干旱预警,对干旱预警产品的分析表明:系统对未来10 d土壤相对湿度预报的决定系数在0. 63～0. 91之间,均方根误差在5. 6%～18. 2%之间,预报时效越近,准确率越高。从不同的干旱等级预测准确率看,对于干旱等级较高的重旱和特旱预报准确率较高,轻旱和中旱的预报准确率略低。该系统基本满足冬小麦干旱预警需求,对国家级农业气象部门大范围农业干旱监测和预警业务是有益的补充。     

Effects of carvedilol on murine viral myocarditis

AIM: To observe the effects of carvedilol on murine viral myocarditis model. METHODS: A total of 188 inbred male BALB/c mice of 4-6 weeks were divided into 4 groups: myocarditis group (group C, n=60), metoprolol treatment group (group M, n=60), carvedilol treatment group (group K, n=60), control group (group B, n=8). Myocardial histopathololgic changes were observed. The concentrations of cardiac troponin I (cTn-I) were detected by chemiluminescence immunoassay (CLIA). Western blotting was performed to analyze the contents of phosphorylated p38MAPK in myocardium. RESULTS: Metropolol and carvedilol lightened myocardial histopathololgic changes at acute stage, decreased cTn-I concentrations and myocardial phosphorylated p38MAPK value compared with myocarditis group. Treatment with carvedilol was more effective than treated with metropolol on those indexes. CONCLUSION: Carvedilol protects against viral myocarditis by inhibition of p38MAPK signal transduction pathway through blockade of β1 and β2 adrenergic receptors.     

山茱萸压条繁殖技木试验

山茱萸压条繁殖技术中,对母株主干基部约10 cm处实行双环切,在剪除萌条时留桩(0.5～1 cm),可分别提高萌条数100%和89%;新萌发的枝条埋条前对萌条生根部位环切2圈,可提高压条苗生根数57%.埋条后埋土厚度15～20 cm比埋土厚度10 cm的提高生根数2.75倍;埋条时1 a生枝条压埋生根容易,根数比2 a生枝条多32%;在母株定植时,采取深穴浅栽比大穴浅栽和普通栽植时成苗率都高,可以达93%,成苗平均生根数可以达到31条.     

水库下游居民风险承受能力模糊综合评价

构建了水库下游居民风险承受能力指标体系,采用熵权系数法对风险承受能力进行模糊综合评价.结合陕西省西安市石砭峪水库下游居民调查实例,通过熵权系数法对各评价指标赋值,利用加权平均模糊合成算子得出评价结论,调查地区水库下游居民风险承受能力等级为"中",针对评价结果给出了提高水库下游居民风险承受能力的具体建议.     

岩土工程勘察作业的重要性与方法

重视岩土工程勘察作业,把握技术分析中的每一环节,强调事先指导、过程控制、事后总结。对于提高岩土工程师的专业技术水平,保证岩土工程勘察质量,促进建设工程的顺利开展,能起到事半功倍的效果。基于岩土工程勘察工作的重要性,从实际的工作经验出发,就勘察前期、中期和后期如何做好岩土工程勘察的外业工作进行了深入探讨,并提出了具体的建议。     

2株鲤鱼源乳酸菌体外抗逆性研究

对2株鲤鱼源乳酸菌作为益生菌候选菌株进行体外抗逆性试验。通过耐受胆盐、耐受pH 值、耐受蛋白酶和耐高温等试验, 对这2株乳酸菌进行研究。其中在胆盐耐受性试验中, YL- 1和YL- 7在胆盐浓度0. 4%、培养4 h时仍有30%以上的存活率; 在pH 值耐受性试验时, 2株乳酸菌均可在一定的酸性和碱性条件下存活; 胰蛋白酶对2株菌的存活率没有影响; 在70e 的高温中, 2株乳酸菌均有一定的耐受性。     

Expression of phenylalanine ammonia-lyase cDNA from rice in E. coli BL21DE3

AIM: To study the expression and its kinetics of rice phenylalanine ammonia-lyase gene encoding into E. coli as the basis of treatment for phenylketouria. METHODS: The phenylalanine ammonia-lyase-1-cDNA(rPAL-1-cDNA) from rice was recombined into E. coli high expression vector pET-28c and transformed into E. coli host strain BL21DE3. Engineering bacteria was then inducted by isopropyl-β-D-thiogalactoside (IPTG) for 1, 3, 5, 7 hours, in order to obtain high level expression. RESULTS: After induction, the expression level of fusion protein was 21.40%, 30.60%, 35.40%, 35.43% respectively. The fusion protein exhibited a band of 78.6 kD on SDS-PAGE analysis, but was not found in controls.The target protein was mainly existed in the form of inclusion body. CONCLUSION:Rice PAL gene expressing E. coli was established by gentic engineering technique.     

Curcumin attenuates rat lung ischemia/reperfusion injury by interfering with autophagy

AIM To investigate the role of curcumin （CUR） in lung ischemia/reperfusion （I/R） injury （LIRI） and its relationship with autophagy. METHODS 40 SD rats were randomly divided into sham operation （sham） group, I/R group, solvent （DMSO） group, CUR group and CUR+rapamycin （CUR-Rap） group. The rats were intraperitoneally injected with normal saline, DMSO, CUR or CUR+Rap before operation. After the rat LIRI model was established, the lung tissues were taken to measure W/D, TLW, IAR, and the contents of SOD and MDA were also measured to indicate the oxidative stress level. Light and electron microscopes were used to observed the morphology and ultrastrucure of lung tissues. The expression levels of autophagy-related proteins were determined by Western blot to evaluate autophagy levels. RESULTS Compared with sham group, wet weight/dry weight （W/D）, total lung water （TLW）, injured alveoli rate（IAR） and malondialdehyde （MDA） content in all other groups were increased, superoxide dismutase （SOD） activity was decreased, the levels of autophagy were increased （P<0.05）, and lung tissue injury and cell ultrastructural damage were aggravated in CUR group. Compared with DMSO group, W/D, TLW and IAR and MDA content were decreased, SOD activity was decreased, autophagy levels were also decreased （P<0.05）, and lung tissue and cell ultrastructural damage were attenuated. Compared with CUR group, W/D, TLW, IAR and MDA content were increased, SOD activity declined, the autophagy levels were increased （P<0.05）, and damage of lung tissues and cells were more serious in CUR-Rap group. CONCLUSION Curcumin attenuates the lung I/R injury in rats, and its mechanism may be related to the reduction of oxidative stress and the inhibition of autophagy.     

苏粳815机插高产形成特点及其配套精确定量栽培技术

通过综合高产试验和机插行株距规格与氮肥组合试验,分析了苏粳815高产形成的特点,明确了其高产产量结构及其形成的主要生育指标,提出了目标产量650~700 kg/667 m~2相配套的精确定量栽培技术。     

中药蟾酥的研究概况

蟾酥作为我国传统中药,其所含化学成分较复杂,具有强心、抗肿瘤、抗炎、麻醉止痛等多种作用,临床应用广泛且疗效显著。本文从蟾酥的化学成分、药理作用及其作用机制、临床应用和剂型等方面进行综述,为其深入的研究提供参考。