The Evaluation System of Agent of Government Investment Project Based on AFD

According to analyses of the existing problems in the evaluation of agent of government investment project, combining with the characters of the substituted construction system of the government invested project and the project management, a performance evaluation index system of the agent of government invested project was constructed, fdlowing the basic principles of the index settlement. It introduces the AHP, the fuzzy evaluation mode, and the ADF comprehensive evaluation mode based on DEA, and gives some practical examples.     

2009年甘蔗实生苗试验家系评价初步结果

对30个甘蔗杂交组合开展家系评价研究的结果发现,各个组合之间的茎径差异极限著.而父本之间则在茎径、田间锤度上的差异分别达到显著或极限著水平.其中,CP94-1100作为父本.其相关杂交组合实生苗11月的平均田间锤度达18.2%、名列第1位,其次为粤糖91-976,而粤糖00-236,粤糖93-159和ROC26居最后;...     

七星瓢虫成虫对狭冠网蝽的捕食功能反应研究

在实验室自然温度条件下,初步研究了七星瓢虫(Coccinella septempunctata L.)对狭冠网蝽 (Stephanitis anagustata Bu.sp.nov)若虫的捕食功能反应。结果表明,七星瓢虫成虫对狭冠网蝽若虫密度的功能反应属于Ⅱ型,可用Holling圆盘方程拟合;随着狭冠网蝽若虫密度的增大,七星瓢虫成虫的捕食量也逐渐增加,其最大捕食量为370．4头;在供试的狭冠网蝽若虫、云杉大蚜和杉针黄叶甲3种猎物中,七星瓢虫成虫对狭冠网蝽若虫均表现出较强的选择性;当七星瓢虫与狭冠网蝽的数量同比例增加时,七星瓢虫个体间的相互干扰系数为0．076 9; 当狭冠网蝽数量不变而七星瓢虫数量增加时,七星瓢虫个体间的相互干扰系数为0．694 0。     

芦荟真空冷冻干燥特性的试验研究

为提高芦荟冷冻干燥速率和品质,在通过物理的方法获得芦荟的共晶点温度的基础上,单因素试验研究干燥室压力、加热搁板温度、预冻速度和物料厚度对芦荟的含水量和冷冻干燥时间的影响。利用芦荟质量的模糊综合评判法,获得芦荟的冷冻干燥的较优工艺参数范围:芦荟切片厚度5～6mm,干燥室压力100～108Pa,加热搁板温度36～38℃,预冻速率-0.40～-0.45℃/min。     

鸡新城疫Ⅰ系疫苗诱导鸡IL-2基因转录及cDNA克隆的研究

应用RT-PCR方法从鸡新城疫 系疫苗接种鸡胚的脾淋巴细胞中扩增出IL-2基因cDNA。结果表明,以鸡新城疫 系疫苗稀释50倍或100倍接种10日龄鸡胚,培养48h的脾脏提取mRNA的扩增效果最好。测序结果显示,所扩增片段的核苷酸序列及其所编码的氨基酸序列,与已发表的IL-2基因的同源性分别为97.7%～99.8%和95.1%～99.3%。其中第8位、68位和130位氨基酸(分别是L,V和S)的变异是其他品种鸡所没有的。     

某羊场和田羊与策勒黑羊无浆体季节性感染PCR检测

为了解和田羊和策勒黑羊无浆体季节动态感染情况,分别于2015年冬至、2016年春分、夏至和秋分,在策勒县某牧业合作社采集和田羊和策勒黑羊血液样本共120份,应用PCR对无浆体的MSP4基因和16SrRNA基因进行扩增。结果发现,检测绵羊的无浆体总阳性率为99.2%(119/120),以绵羊无浆体(Anaplasma ovis)和嗜吞噬无浆体(A.phagocytophilum)混合感染为主,占阳性样本比例为61.3%(73/119),A.ovis和A.phagocytophilum占阳性样本比例分别为31.1%(37/119)和7.6%(9/119)。在不同季节,和田羊和策勒黑羊无浆体均普遍感染,差异不显著(P>0.05)。结果显示,新疆策勒县某羊场绵羊的无浆体病全年均可流行,应引起人们重视。     

Effect of CHOP expression knock-down on apoptosis of renal tubular epithelial cells

AIM:To study the effect of C/EBP homologous protein (CHOP) on the apoptosis of renal tubular epithelial HK2 cells. METHODS:The serum mRNA levels of CHOP in the patients with acute kidney injury and healthy controls were detected by qPCR. In vitro, renal tubular epithelial HK2 cells were divided into control group, negative group (transfected with negative control siRNA), si-CHOP group (transfected with CHOP siRNA), and induced by transforming growth factor-β1 (TGF-β1). The viability of the cells was measured by MTT assay, and the apoptotic rate was analyzed by flow cytometry. The protein levels of nuclear antigen Ki-67, proliferating cell nuclear antigen (PCNA), caspase-3 and cleaved caspase-3 were determined by Western blot. RESULTS:Compared with the healthy controls, the serum mRNA levels of CHOP in the patients with acute kidney injury were increased significantly (P<0.05). Transfection with CHOP siRNA significantly decreased the expression of CHOP in the renal tubular epithelial HK2 cells (P<0.05). Knock-down of CHOP expression by siRNA significantly increased the viability of renal tubular epithelial HK2 cells (P<0.05), decreased the apoptotic rate (P<0.05), increased the expression of Ki-67 and PCNA (P<0.05), and down-regulated the protein level of cleaved caspase-3 (P<0.05). CONCLUSION:The serum mRNA levels of CHOP were increased in the patients with acute kidney injury. Knock-down of CHOP expression inhibits the apoptosis of renal tubular epithelial cells by regulating the expression of proliferation-and apoptosis-related proteins.     

FLUENT软件及其在植保机械方面的应用

FLUENT软件是流体力学中通用性较强的商业软件之一，由于FLUENT软件的应用，节省了大量的人力和物力。对实际应用有重大的指导作用。为此，通过简述FLUENT在植保机械方面的应用实例，提出了FLUENT在植保机械方面的发展前景。     

鸡BPI氨基端的基因克隆和鉴定

应用RT-PCR技术,参照GenBank报道的预测序列,从鸡多形核白细胞(PMN)mRNA中扩增出杀菌/通透性增加蛋白(BPI) 氨基端95个氨基酸的基因片段,并与pMD18-T连接,构建BPI氨基端的基因重组体,进行酶切鉴定和序列测定.结果表明,获得了BPI N端长度为284 bp的基因片段,序列分析证实该片段中有3个点突变,但均不在活性中心.     

J亚群禽白血病病毒分离株HLJ09SH01株感染性克隆的构建及其致病性研究

为构建J亚群禽白血病病毒(ALV-J)分离株的感染性克隆并鉴定其对蛋鸡和肉鸡致病力的差异,本研究从黑龙江地区分离鉴定一株ALV-J分离株HLJ09SH01,以其核酸为模板,采用PCR方法分3段扩增其前病毒cDNA,PCR产物经克隆酶切后依次连接,获得一个含有完整ALV-J前病毒cDNA的重组质粒,命名为pBlue-HLJ09SH01。将其转染DF-1细胞,进行病毒拯救。通过间接免疫荧光、禽白血病抗原试剂盒检测及反转录酶活性试剂盒检验,表明拯救的病毒为ALV-J,命名为rHLJ09SH01。将其分别人工接种11日龄蛋鸡和肉鸡鸡胚,孵育出壳后隔离饲养32周。致瘤性试验结果表明,rHLJ09SH01可以导致57.9%的蛋鸡发生肿瘤,83.3%的肉鸡发生肿瘤。表明该感染性克隆具有亲本病毒的致病性。