Effects of glycine on intracellular of free calcium and tumor necrosis factor-α of cardiomyocytes during hypoxia/reoxygenation

AIM: To observe the influence of glycine on intracellular free calcium, the concentration of tumor necrosis factor-α and the survival rate of myocardial cells during hypoxia/reoxygenation (H/R). METHODS: The simulated model of myocardial ischemia-reperfusion with the primary cultured cardiomyocytes of neonatal rats was established, and the cultured cardiomyocytes were divided into seven groups, control group, hypoxia/reoxygenation group, glycine (0.5 mmol/L) plus hypoxia/reoxygenation group, glycine (1.0 mmol/L) plus hypoxia/reoxygenation group, glycine (2.0 mmol/L) plus hypoxia/reoxygenation group, glycine (4.0 mmol/L) plus hypoxia/reoxygenation group, 4.0 mmol/L glycine group. RESULTS: Within certain concentration (0.5-2.0 mmol/L), the glycine could inhibit the calcium overload resulting from hypoxia/reoxygenation injury in cells in a dose-dependent manner with the optimal inhibitory effect at 2.0 mmol/L. Glycine inhibited the secretion of tumor necrosis factor-α from myocardial cells and increased the survival rate of myocardial cells. CONCLUSION: Glycine has a protective effect on hypoxia/reoxygenation myocardial cells, which may be related to inhibiting calcium overload and decreasing the production of tumor necrosis factor-α.     

基于CVM的牧户对减畜政策的受偿意愿分析

研究牧民对草原生态保护补助奖励机制的受偿意愿,对完善新一轮(2016-2020)的草原生态补奖机制具有重要意义。选取甘肃省玛曲县为研究区域,通过条件价值评估法(CVM)调查研究区牧民对当前实施的减畜政策的态度和受偿意愿,初步的统计分析发现,被访牧户中有59.81%的牧户愿意减畜,而40.19%的不愿意减畜的牧户认为当前实施的补偿标准太低,无法弥补因减畜造成的家庭经济损失。通过计量模型分析发现:牧户对减畜的支持态度主要与家庭劳动力负担系数、草场承包规模、联户情况、草场健康状况以及牲畜规模呈正相关,与草场租赁情况呈负相关;而牧户对减畜的受偿意愿额度主要由牧户受教育年限、家庭牧业收入、草场承包规模、牲畜规模以及联户情况等决定;根据CVM法估算了被访牧户的受偿意愿的期望值为E(WTA)=12.61元/亩,而通过Tobit模型计算了被访牧户户均理论受偿意愿值为WTA=12.16元/亩,但在政策实施中玛曲县草畜平衡奖励标准为2.18元/亩,进一步说明现行补偿标准明显偏低。     

益母草对绿壳蛋鸡血液生化指标及激素的影响

将280只50周龄健康、生产性能相近的黑羽绿壳蛋鸡随机均分为4组,每组设5个重复,每个重复14只。对照组饲喂基础日粮,试验组在基础日粮中分别添加0.5%、1.0%、1.5%益母草粉,观察其对黑羽绿壳蛋鸡血清生化指标、血液激素及卵巢的影响。结果表明,与对照组相比,在绿壳蛋鸡日粮中添加1.0%和1.5%益母草粉可显著降低血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL)含量,提高高密度脂蛋白(HDL)、雌二醇(E₂)和促卵泡激素(FSH)含量,增加卵巢相对重量。益母草可以影响脂肪代谢,具有降低血脂的作用,考虑到生产成本问题,最适添加量为1.0%。     

DL-羟基蛋氨酸钙相对于DL-蛋氨酸的生物学效价

试验采用肉仔鸡生产性能和胴体品质指标结合回归模型比较了DL-羟基蛋氨酸钙(DL-MHA-Ca)相对于DL-蛋氨酸(DLM)的生物学效价。选取792只体重相近且健康的AA肉仔鸡,随机分为11个处理,每个处理6个重复。DLM或DL-MHA-Ca按照等摩尔水平(0.03%、0.06%、0.10%、0.15%和0.21%)添加到仅缺乏蛋氨酸的基础日粮中。试验期35 d,分为7~21 d和22~42 d 2个阶段。结果表明:7~21 d和7~42 d,添加蛋氨酸源显著改善了肉仔鸡的体增重、饲料/增重、屠体重、胸肉重、胸肉率、腿肉重和全净膛重(P0.05);在等摩尔基础上,DL-MHA-Ca相对于DLM的生物学效价,7~21 d分别是71%(增重)和66%(饲料/增重);7~42 d则分别为63%(增重)、58%(饲料/增重)、74%(屠体重)、60%(胸肉重)、49%(胸肉率)、71%(腿肉重)和72%(全净膛重)。     

Genetic Analysis and Mapping of TWH Gene in Rice Twisted Hull Mutant

A mutant with twisted hulls was found in a breeding population of rice (Oryza sativa L.). The mutant shows less grain weight and inferior grain quality in addition to twisted hulls. Genetic analysis indicated that the phenotype of mutant was controlled by a single recessive gene (temporarily designated as TWH). To map the TWH gene, an F2 population was generated by crossing the twh mutant to R725, an indica rice variety with normal hulls. For bulked segregant analysis, the bulk of mutant plants was prepared by mixing equal amount of plant tissue from 10 twisted-hull plants and the bulk of normal plants was obtained by pooling equal amount tissue of 10 normal-hull plants. Two hundred and seven pairs of simple sequence repeat (SSR) primers, which are distributed on 12 rice chromosomes, were used for polymorphism analysis of the parents and the two bulks. The TWH locus was initially mapped close to the SSR marker RM526 on chromosome 2. Therefore, further mapping was performed using 50 pairs of SSR primers around the marker RM526. The TWH was delimited between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provide the foundation for further fine mapping, cloning and functional analysis of the TWH gene.     

昆嵛山国家森林公园植物胶植物资源调查研究

通过上山实地采集调查以及实验室标本鉴定,对昆嵛山植物胶植物的种类及形态特征进行统计。结果表明:昆嵛山植物胶植物共计39种,隶属15个科;在此基础上,总结概括了植物胶植物在工业、食品、生物制药等方面的应用并提出了资源开发利用的前景与建议。     

藏绵羊KLF7基因表达特征分析及其过表达对前脂肪细胞增殖分化的影响

【目的】分析藏绵羊Krüppel样因子7(Krüppel-like factor 7,KLF7)基因表达特征,研究过表达该基因对前脂肪细胞增殖及分化的影响。【方法】从藏绵羊脂肪组织中分离前脂肪细胞进行培养及成脂诱导,应用实时荧光定量PCR技术检测KLF7基因在藏绵羊7个组织(大脑、皮下脂肪、肾脏、背最长肌、瘤胃、睾丸和回肠)和前脂肪细胞不同分化阶段(第0、2、4和8天)的mRNA相对表达水平;应用RT-PCR方法从藏绵羊脂肪组织中扩增KLF7基因CDS区序列,并将其连接到pcDNA3.1(+)真核表达载体获得pcDNA3.1-KLF7过表达质粒,转染前脂肪细胞;应用实时荧光定量PCR方法检测脂肪细胞增殖及分化标志基因mRNA表达水平;采用EdU和CCK-8方法分别检测过表达KLF7基因对EdU阳性细胞数和细胞活力的影响;采用油红O染色检测过表达KLF7基因后脂肪细胞脂滴生成量。【结果】KLF7基因在藏绵羊7个组织中均有表达,其中在大脑中的表达量最高,其次为皮下脂肪和肾脏,均显著高于其他组织(P<0.05);诱导分化第2、4和8天脂肪细胞mRNA表达量均显著高于分化前(P＜0.05),且分化第2天表达量最高;pcDNA3.1-KLF7过表达质粒转染前脂肪细胞2 d后显著或极显著抑制增殖标志基因CDK4、CyclinB1和CyclinD1的表达水平(P＜0.05;P＜0.01),极显著降低细胞活力及EdU阳性细胞数量(P＜0.01);pcDNA3.1-KLF7过表达质粒转染前脂肪细胞,诱导分化8 d后,脂肪细胞分化标志基因PPARγ、Glut4和ELOVL6的mRNA相对表达水平显著或极显著下调(P＜0.05;P＜0.01),且脂质沉积极显著减少(P＜0.01),表明过表达KLF7基因可抑制藏绵羊前脂肪细胞增殖及分化。【结论】KLF7基因在藏绵羊多个组织中广泛表达,且大脑、皮下脂肪、肾脏中表达量较高;诱导分化后脂肪细胞表达量显著高于分化前,且分化第2天表达量最高;过表达KLF7基因可抑制藏绵羊前脂肪细胞的增殖及分化。试验结果为阐明藏绵羊脂肪沉积的分子调控机制提供了基础数据。     

广东果树上17种拟茎点霉的RAPD分析

 自320个随机引物中筛选出适于拟茎点霉属真菌种间亲缘关系分析的15个随机引物,并优化了RAPD分析的扩增体系,在此基础上,对广东果树上17种拟茎点霉进行了RAPD分析。各菌株间的Nei相似系数UPGMA法聚类结果表明:来源于不同地区的2个Phomopsis mangiferae Ahmad菌株和2个P.macadami Z.D.Jiang et P.K.Chi菌株都分别以0.636和0.589的相似系数两两首先聚在一起,而不同的种则只在小于0.54的相似系数范围内聚类,体现了种间及种内的亲缘关系差异程度;聚类群与寄主植物不具相关性,同种植物上的不同拟茎点霉,即使是分自相同寄生部位也不能聚在一类;支持形态学上将生于柑桔枝和黄皮茎、沙梨叶和果、杨梅叶和枝以及同是生于龙眼叶的共8个拟茎点霉分别鉴定为不同的种,而不支持将P. cytosporella Penz.et Sacc.与P. mangiferae合并为一个种的观点;RAPD技术可作为拟茎点霉属真菌种间的亲缘关系分析的重要手段。     

Irrigation scheduling based on yield and quality in Bromus inermis北大核心CSCD

A suitably calculated irrigation regime can improve the productivity of artificial grassland,improve the quality of pasture and enhance water use efficiency in drier areas. In this research we compared the effects of two irrigation regimes（regulated deficit irrigation at different growth stages,I1;regulated deficit irrigation over the whole growth period ,I2）on yield and quality of Bromus inermis in the Hexi Corridor. Irrigation scheduling was optimized by considering the results of field trials and simulations of irrigation scheduling in typical dry years to provide a theoretical basis for local production practices. The results showed that：The yield of B. inermis in I1 initially increased and then decreased with successive increases in the amount of water applied,while in I2 B. inermis yield showed an increasing trend across the range of irrigation rates tested. In both irrigation regimes,the crude protein content of B. inermis gradually decreased with increasing water supply,while the acid detergent fiber and neutral detergent fiber contents increased somewhat. Compared with I2,B. inermis yield was increased by 23.11% on average and crude protein content by 6. 09% on average in I1;therefore,the I1 irrigation regime was superior. Based on the field trial combined with the simulation of irrigation scheduling in a typical dry year,it was found that a treatment designated ［greening stage （75%-85% θFC）,jointing stage （65%-85% θFC）,and tasseling stage （65%-85% θFC）,I1DF1］,irrigation frequency of 12 times,and a total irrigation amount of 521. 76 mm provided water-saving benefits and stable yield in B. inermis in the Hexi Corridor. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved.     

猪捷申病毒Swine/CH/IMH/03分离株细胞适应性和致病性研究

为确定猪捷申病毒(PTV)Swine/CH/IMH/03株的易感细胞系及其致病性,本实验将该病毒接种不同细胞,通过细胞病变、电子显微镜观察和PCR技术进行鉴定。并进一步将该病毒回归本动物鉴定其致病性。试验结果表明,PTV Swine/CH/IMH/03对IB-RS-2和PK-15细胞系敏感,能够在IB-RS-2和PK-15细胞系中增殖。致病性试验结果显示,Swine/CH/IMH/03分离株能够导致实验猪出现神经症状,剖检可见脑、肾、脾、肠道发生出血,病理组织学检查可见脑实质内胶质细胞浸润、肾脏间质小血管淤血、脾淋巴细胞减少、小肠伴有出血及淋巴细胞浸润等病理变化,对仔猪具有强致病性。