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81.
Catherine R. Back Henry L. Stennett Sam E. Williams Luoyi Wang Jorge Ojeda Gomez Omar M. Abdulle Thomas Duffy Christopher Neal Judith Mantell Mark A. Jepson Katharine R. Hendry David Powell James E. M. Stach Angela E. Essex-Lopresti Christine L. Willis Paul Curnow Paul R. Race 《Marine drugs》2021,19(2)
To tackle the growing problem of antibiotic resistance, it is essential to identify new bioactive compounds that are effective against resistant microbes and safe to use. Natural products and their derivatives are, and will continue to be, an important source of these molecules. Sea sponges harbour a diverse microbiome that co-exists with the sponge, and these bacterial communities produce a rich array of bioactive metabolites for protection and resource competition. For these reasons, the sponge microbiota constitutes a potential source of clinically relevant natural products. To date, efforts in bioprospecting for these compounds have focused predominantly on sponge specimens isolated from shallow water, with much still to be learned about samples from the deep sea. Here we report the isolation of a new Micromonospora strain, designated 28ISP2-46T, recovered from the microbiome of a mid-Atlantic deep-sea sponge. Whole-genome sequencing reveals the capacity of this bacterium to produce a diverse array of natural products, including kosinostatin and isoquinocycline B, which exhibit both antibiotic and antitumour properties. Both compounds were isolated from 28ISP2-46T fermentation broths and were found to be effective against a plethora of multidrug-resistant clinical isolates. This study suggests that the marine production of isoquinocyclines may be more widespread than previously supposed and demonstrates the value of targeting the deep-sea sponge microbiome as a source of novel microbial life with exploitable biosynthetic potential. 相似文献
82.
83.
Summary The chloroplast DNA of 27 potato cultivars and 2 wild species has been examined by Southern blotting using probes representing
the entire chloroplast genome of barley. 16% of the probe × enzyme combinations detected polymorphism between the potato cultivars.
Cvs Maris Piper, Croft, Shelagh, Stormont Enterprise and Estima and the wild species could be distinguished from the other
cultivars by their chloroplast DNA restriction patterns. These results are discussed in relation to cytoplasmic diversity
and potato improvement. 相似文献
84.
Precision Agriculture - Soil acidification is a growing problem in semi-arid agroecosystems. In the state of Montana, USA, soil pH levels below 5.5 have been documented in nearly half of the... 相似文献
85.
D G Powell 《Veterinary Clinics of North America: Equine Practice》2000,16(3):389-403, vii
Examples of equine disease surveillance at the local, regional, national and international level are discussed in this article. Reporting systems at each level are also considered, and the increasing importance of laboratory confirmed diagnoses is emphasized. The need to develop national disease reporting systems is addressed, particularly with respect to conforming to international trading standards. 相似文献
86.
Populations of the green peach aphid,Myzus persicae (Sulzer), and the potato aphid,Macrosiphum euphorbiae (Thomas), were observed on 27 named varieties of Irish potatoes in the Yakima Valley of Washington intermittently from 1947 through 1968. The Epicure, Great Scot, and B5141-6 varieties produced relatively fewer green peach aphids than other varieties tested; Bliss Triumph and Red Pontiac produced relatively fewer potato aphids. Except for Doebay Red, the red-skinned varieties, Bliss Triumph, Red McLure, Norland, and Earnly Rose, were extremely to moderately productive of the green peach aphid. No correlation was apparent between abundance of aphids and the specific gravity or yield of tubers. 相似文献
87.
88.
D. R. Morris B. Glaz G. Powell C. W. Deren G. H. Snyder R. Perdomo 《Journal of plant nutrition》2013,36(9):1511-1523
ABSTRACT Most of the sugarcane (interspecific hybrids of Saccharum sp.) production in Florida is on organic soils. Supplemental phosphorus (P) fertilizer is often applied for optimum yields, but producers are required to reduce P levels in farm drainage waters. The objectives of this study were to relate optimum leaf P tissue concentration with yield in organic soil, and to determine optimum leaf sampling dates during the summer. Eight genotypes were planted at two locations, eight additional genotypes were planted at a third location, and eight more genotypes were planted at a fourth location. Crops were grown for three years. Measurements of leaf P concentration were repeated during growth seasons and over crop years for a total of six sampling dates at each location. Three fertilizer P treatments (0, 24, and 48 kg ha?1 yr?1) were applied to all genotypes at each location. Leaf samples were partitioned into early-, mid-, and late-summer dates. Early-leaf samples had the widest range in leaf P concentrations compared with mid- and late-season leaf samples. Correlation analyses of yield vs. leaf P concentration across all treatments in early- and mid-summer were statistically significant (P < 0.05), but coefficients were low (r = 0.14 and 0.26, respectively). No consistent relationship across locations described the effect of leaf P tissue concentration on yield. Leaf P concentrations could not provide accurate P fertilization rates that will give maximum sugarcane yields and prevent over-fertilization of P. The highest potential for relating leaf P concentrations with yield appears to be from early leaf samples. 相似文献
89.
The distribution of postganglionic autonomic nerve fibers in the lacrimal gland and gland of the third eyelid of dogs was studied by use of histochemical techniques. Adrenergic nerve distribution was identified by use of the sucrose-potassium phosphate-glyoxylic acid technique. A loose network of adrenergic nerves was found throughout the interstitium around acini and blood vessels and in vessel walls. Acetylcholinesterase staining was used to identify cholinergic nerve fibers. A cholinergic distribution pattern was found, although the cholinergic innervation appeared more dense than the adrenergic. In the gland of the third eyelid, mucus-secreting lobules and lipid-secreting lobules appeared to be equally innervated by parasympathetic fibers. These lobules could not be differentiated when the sucrose-potassium phosphate-glyoxylic acid technique was used. The techniques used in this study could not demonstrate whether direct contact was made by either cholinergic or adrenergic nerve fibers with secretory or myoepithelial cells. The presence of both nerve fiber types around acini suggests an interrelationship between the sympathetic and parasympathetic nervous system in lacrimal gland secretion in dogs. 相似文献
90.
Comparative survival and growth performance of European lobster larvae,Homarus gammarus,reared on dry feed and conspecifics 下载免费PDF全文
Adam Powell James Hinchcliffe Kristina Sundell Nils‐Gunnar Carlsson Susanne P. Eriksson 《Aquaculture Research》2017,48(10):5300-5310
A bottleneck of crustacean larval culture concerns nutrition and associated cannibalism in communal rearing systems, which impact on larval survival, development and growth. For early‐stage European lobster, Homarus gammarus larvae, feeding ecology and body composition are largely unknown. We initiated four progressive feeding experiments (novel feed types, feeding regime and feed size and cannibalism effects) on growth and survival, to inform and update husbandry protocols. Performance of larvae offered a dry commercial feed was not significantly different compared with a conventional wet plankton feed of the same ration and size grade (both within 600–1,000 μm). Further experiments found that the same ration of dry feed offered six times daily improved development and growth, over the conventional regime of three times daily. Small‐grade dry feed (particles: 250–360 μm) improved larval performance compared with a larger feed (360–650 μm). Larvae were also fed different proportions of dry feed and/or conspecifics in both communal and individual rearing systems (the latter preventing cannibalism via segregation). Individually reared larvae, fed only dead conspecifics, displayed the greatest survival (80%) to postlarvae. This underlines the impact of cannibalism on survival and nutrition in H. gammarus larviculture. A final experiment analysed H. gammarus zoea 1 composition, identifying deficiencies in ash and carbohydrate in lobster feeds. This suggests a need for a species‐specific, formulated dry feed for H. gammarus larviculture. Our research represents the first investigation of H. gammarus larval composition and dietary requirements and highlights decreased growth potential associated with providing nutrition solely from generic commercial feed. 相似文献