全文获取类型
收费全文 | 132329篇 |
免费 | 7393篇 |
国内免费 | 92篇 |
专业分类
林业 | 5381篇 |
农学 | 4269篇 |
基础科学 | 831篇 |
17035篇 | |
综合类 | 21476篇 |
农作物 | 5393篇 |
水产渔业 | 6688篇 |
畜牧兽医 | 67959篇 |
园艺 | 1800篇 |
植物保护 | 8982篇 |
出版年
2020年 | 1177篇 |
2019年 | 1375篇 |
2018年 | 2031篇 |
2017年 | 2330篇 |
2016年 | 2095篇 |
2015年 | 1742篇 |
2014年 | 2224篇 |
2013年 | 5290篇 |
2012年 | 4114篇 |
2011年 | 5093篇 |
2010年 | 3245篇 |
2009年 | 3389篇 |
2008年 | 4971篇 |
2007年 | 4666篇 |
2006年 | 4353篇 |
2005年 | 4088篇 |
2004年 | 3850篇 |
2003年 | 3973篇 |
2002年 | 3542篇 |
2001年 | 4150篇 |
2000年 | 4252篇 |
1999年 | 3372篇 |
1998年 | 1474篇 |
1997年 | 1387篇 |
1996年 | 1288篇 |
1995年 | 1529篇 |
1994年 | 1358篇 |
1993年 | 1231篇 |
1992年 | 2584篇 |
1991年 | 2700篇 |
1990年 | 2591篇 |
1989年 | 2611篇 |
1988年 | 2368篇 |
1987年 | 2348篇 |
1986年 | 2427篇 |
1985年 | 2340篇 |
1984年 | 1881篇 |
1983年 | 1677篇 |
1982年 | 1149篇 |
1979年 | 1748篇 |
1978年 | 1397篇 |
1977年 | 1151篇 |
1976年 | 1212篇 |
1975年 | 1292篇 |
1974年 | 1500篇 |
1973年 | 1514篇 |
1972年 | 1405篇 |
1971年 | 1304篇 |
1970年 | 1251篇 |
1969年 | 1283篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
211.
Serum amyloid A protein concentration measured by radial immunodiffusion in Abyssinian and non-Abyssinian cats 总被引:1,自引:0,他引:1
S P DiBartola J A Reiter J B Cornacoff G J Kociba M D Benson 《American journal of veterinary research》1989,50(8):1414-1417
Serum amyloid A (SAA) protein concentration was determined by use of radial immunodiffusion (RID) in 4 groups of cats: Abyssinian cats with amyloidosis, healthy Abyssinian cats without clinical evidence of amyloidosis, hospitalized non-Abyssinian cats, and clinically normal non-Abyssinian cats. Mean SAA concentration in Abyssinian cats with amyloidosis was significantly (P = 0.05) higher than mean SAA concentration in healthy Abyssinian cats without clinical evidence of amyloidosis and in hospitalized non-Abyssinian cats. Mean SAA concentration in clinically normal non-Abyssinian cats was significantly (P = 0.05) lower than mean SAA concentration in healthy Abyssinian cats without clinical evidence of amyloidosis and in hospitalized non-Abyssinian cats. Affected and healthy Abyssinian cats, however, could not reliably be distinguished on the basis of SAA concentration, because of the wide range of SAA values in these 2 groups of cats. 相似文献
212.
Effects of epidural xylazine on EEG responses to surgical stimulation during isoflurane anaesthesia in dogs 总被引:1,自引:0,他引:1
K. A. Otto S. Piepenbrock B. Rischke C. Bötel† 《Veterinary anaesthesia and analgesia》1997,24(1):33-38
The effects of epidural administration of 250 μg/kg xylazine on EEG responses to surgical stimulation of 5 different intensities were evaluated during isoflurane anaesthesia for an experimental orthopaedic procedure in dogs. The dogs were assigned randomly to one of 2 treatment groups receiving either xylazine (n = 4) or equal volumes of sterile water (n = 4) (control group) epidurally. Intense surgical stimulation during removal of a bone graft from the dorsoiliac spine of the ileum was associated with a significantly (P = 0.0339) higher increase in EEG alpha/delta ratio after epidural administration of sterile water than after epidural injection of 250 μg/kg of xylazine. In addition, the preincision baseline values for 80% spectral edge frequency were significantly (P = 0.0339) lower in the xylazine group compared to control dogs. Our results suggest that epidural administration of 250 μg/kg of xylazine during orthopaedic procedures in dogs exerts antinociceptive effects which may be in part mediated by a supraspinal effect of xylazine. 相似文献
213.
214.
T Ishida A Taniguchi S Matsumura T Washizu I Tomoda 《Veterinary immunology and immunopathology》1992,35(1-2):15-22
Eleven feline immunodeficiency virus (FIV) infected asymptomatic carrier (AC) cats were observed for 2 years for development of acquired immunodeficiency syndrome (AIDS). Four of the 11 (36.4%) showed progression of the clinical stage. Persistent generalized lymphadenopathy was noted in three cats as the first sign of illness after the AC phase, while the other showed lymphadenopathy with signs of AIDS-related complex. In all four cats the AIDS-related complex stage lasted for 10 months or longer, and two showed progression of the disease into AIDS. The two cats showing AIDS illnesses died within approximately 1 year after they had developed persistent generalized lymphadenopathy. Pathology confirmed the diagnosis of AIDS characterized by the presence of depletion lesions in the lymphoid organs, and of severe infections of an opportunistic nature. The overall mortality of FIV infected AC cats during a 2 year period was two out of 11 (18.2%). These cats showed decreased concanavalin A mitogen response of peripheral blood mononuclear cells as the disease progressed. 相似文献
215.
216.
Sera from 74,502 cattle from 3087 farms in England and Wales were tested for the presence of antibodies against Hypoderma bovis in the spring of 1988. Twenty-nine positive sera were identified on 18 premises and these animals were treated; an examination of 6030 sera taken from 108 neighbouring herds identified another 17 seropositive animals on 10 farms in Devon, Cornwall, Lancashire, Shropshire and Powys, indicating that these counties still harbour populations of warble fly. 相似文献
217.
A W Mills 《Veterinary Clinics of North America: Small Animal Practice》1992,22(6):1297-1307
The impact and relevance of dentistry in a general feline practice is detailed. Hospital policy and protocol are described for client education, preanesthetic evaluation, dental procedures, and recall programs. A discussion of commonly observed feline dental problems is included. 相似文献
218.
J P Dubey A N Hamir C A Hanlon C E Rupprecht 《Journal of the American Veterinary Medical Association》1992,200(4):534-536
Serum samples from 427 raccoons (93 from Pennsylvania, 45 from New Jersey, 72 from South Carolina, 68 from Virginia, 30 from Iowa, and 119 from Ohio) were evaluated for Toxoplasma gondii antibodies in dilutions of 1:25, 1:50, and 1:500. The distribution of T gondii antibody titers was less than 1:25 for 212 raccoons (49.6%), 1:25 for 34 raccoons (7.9%), 1:50 for 117 raccoons (27.4%), and greater than or equal to 1:500 for 64 raccoons (14.9%). Tissue cysts were seen in the liver, and tachyzoites were in the brain of a raccoon with abnormal neurologic signs and concurrent infection with canine distemper virus. Organisms in the liver were stained with anti-T gondii serum, and the raccoon had a T gondii titer of 1:160 in the agglutination test. 相似文献
219.
Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies was reactive in the immunofluorescent detection of BFD virus-infected cells. These antibodies immunoprecipitated intact virions and specifically recognized the major capsid protein, VP1, of the dissociated virion. The MAbs were found to preferentially recognize native BFD virus capsid protein when compared with denatured virus protein. These MAbs were capable of detecting BFD virus protein in chicken embryonated cell-culture lysates by dot-blot analysis. 相似文献
220.
A de la Concha-Bermejillo C E Schore C A Dangler C C de Mattos C A de Mattos B I Osburn 《American journal of veterinary research》1992,53(12):2245-2250
A slot blot hybridization technique was applied for detection of bluetongue virus (BTV) in blood mononuclear cells (BMNC) obtained from cattle with experimentally induced infection. This technique lacked sensitivity to detect the viral nucleic acid directly in clinical specimens. When aliquots of mononuclear cells from these cattle were cultivated in vitro for 10 days to amplify virus titer, only 33.3% of the samples collected during viremia gave a positive signal in the slot blot hybridization format. By contrast, results for 34.3% of noncultured and 63.3% of cultured mononuclear cell samples collected during viremia were positive by immunofluorescence. The average number of infected cells, as detected by immunofluorescence in the noncultured mononuclear cell samples, was 1 to 5/300,000, and was usually > 10/300,000 in the cultured cell samples. Virus was isolated from all postinoculation blood samples obtained from 4 heifers that were seronegative at the time of inoculation, but was not isolated from any of the preinoculation samples, or from any of the postinoculation samples obtained from 2 heifers that were seropositive at the time of inoculation. When virus isolation was attempted from separated mononuclear cells in 2 heifers, 43.7% of the noncultured and 87.5% of the cultured samples had positive results. 相似文献