Testicular ischaemic necrosis as a cause of equine cryptorchidism

Cryptorchidism is a fairly common pathology presented to equine surgical facilities with the cryptorchid testicle most commonly located in the abdomen or ipsilateral inguinal canal. The causes of cryptorchidism are not known, but testicular abnormalities have been suggested. Monorchidism as a cause of maldescent of one or both testicles is rare and is hypothesised to be the result of a vascular insult, similar to testicular regression in man. This case report details laparoscopic abdominal exploration of a cryptorchid horse and identification of an abnormal testicular remnant affected by ischaemic necrosis.     

Retention of native woody vegetation on farms in Australia: management considerations,planning guidelines and information gaps

Australia's forests and woodlands have been extensively cleared since European settlement. Cropping regions have the least retained native vegetation, with the greatest depletion of forests and woodlands being observed in Western Australia and South Australia. Salinisation, erosion and landslides have resulted from excessive clearing and inappropriate tree removal from particular units within the landscape. Major problems in cropping regions are the dramatic reduction in total woody cover and the fragmentation of retained vegetation into isolated remnant patches which tend to be very small (<10–15 ha). Those states with little remnant vegetation have legislation which restricts further clearing and the main emphasis is on managing remnant patches for conservation. For those states with a large proportion of woody vegetation uncleared, the main priority is to ensure that past mistakes are not repeated and that any vegetation clearance is based upon sound ecological principles.Throughout Australia, the condition of the remaining vegetation is of great concern and management guidelines for both conservation and production are being sought. Clearing may lead directly to soil erosion, but often the cause of erosion is inappropriate post-clearing management. Overgrazing is a major cause of vegetation and soil degradation, particularly in semi-arid regions.An understanding of the complex inter-relationship between woody vegetation, grazing and fire is necessary if 1) flora and fauna are to be conserved; 2) hydrological balance is to be maintained; and 3) rural industries are to remain viable. Planning at the catchment level is necessary to implement strategies to meet conservation and hydrological goals. Within that framework, individual property planning must include consideration of the area of native woody vegetation to be retained and its configuration. In many cases, the purpose of retention will influence the decisions of where and how native vegetation should be retained.     

The use of pestivirus antigen ELISA currently available for the detection of hairy shaker disease/border disease virus in sheep

AIMS: To estimate the number of cases of scrapie that would occur in sheep of different prion protein (PrP) genotypes if scrapie was to become established in New Zealand, and to compare the performance of two commercially available, rapid ELISA kits using ovine retro-pharyngeal lymph nodes (RLN) from non-infected and infected sheep of different PrP genotypes.

METHODS: Using published data on the distribution of PrP genotypes within the New Zealand sheep flock and the prevalence of cases of scrapie in these genotypes in the United Kingdom, the annual expected number of cases of scrapie per genotype was estimated, should scrapie become established in New Zealand, assuming a total population of 28 million sheep. A non-infected panel of RLN was collected from 737 sheep from New Zealand that had been culled, found in extremis or died. Brain stem samples were also collected from 131 of these sheep. A second panel of infected samples comprised 218 and 117 RLN from confirmed scrapie cases that had originated in Europe and the United States of America, respectively. All samples were screened using two commercial, rapid, transmissible spongiform encephalopathy ELISA kits: Bio-Rad TeSeE ELISA (ELISA-BR), and IDEXX HerdChek BSE-Scrapie AG Test (ELISA-ID).

RESULTS: If scrapie became established in New Zealand, an estimated 596 cases would occur per year; of these 234 (39%) and 271 (46%) would be in sheep carrying ARQ/ARQ and ARQ/VRQ PrP genotypes, respectively. For the non-infected samples from New Zealand the diagnostic specificity of both ELISA kits was 100%. When considering all infected samples, the diagnostic sensitivity was 70.4 (95% CI=65.3–75.3)% for ELISA-BR and 91.6 (95% CI=88.2–94.4)% for ELISA-ID. For the ARQ/ARQ genotype (n=195), sensitivity was 66.2% for ELISA-BR and 90.8% for ELISA-ID, and for the ARQ/VRQ genotype (n=107), sensitivity was 81.3% for ELISA-BR and 98.1% for ELISA-ID.

CONCLUSIONS: In this study, the ELISA-ID kit demonstrated a higher diagnostic sensitivity for detecting scrapie in samples of RLN from sheep carrying scrapie-susceptible PrP genotypes than the ELISA-BR kit at comparable diagnostic specificity.

CLINICAL RELEVANCE: The diagnostic performance of the ELISA-ID kit using ovine RLN merits the consideration of including this assay in the national scrapie surveillance programme in New Zealand.