Variation of seed characters in selectedVicia species

Summary The variability of 19 seed characters has been examined for 52 species of the genusVicia. Seed circumference, relative hilum length, hilum shape and lens position could be confirmed as the most relevant characters for grouping. By cluster analyses based upon them the material has been classified into 11 groups. Discriminant analyses gave 25 % misclassifications when all 19 variables were subjected to the analysis for the determination of individual seeds. A key for determination of the species has been proposed mainly by means of quantitative seed characters; dendrograms illustrate the result of the cluster analyses and seed diagrams show schematically important seed characters.

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Die Variabilität von Samenmerkmalen bei ausgewähltenVicia-Arten

Zusammenfassung Es wurde die Variabilität von 19 Samenmerkmalen bei 52 Arten der GattungVicia geprüft. Für eine Gruppenbildung bestätigten sich dabei der Samenumfang, die relative Nabellänge, die Nabelform und die Lage der Chalaza als besonders geeignet. Bei einer auf diesen Merkmalen aufbauenden Cluster-Analyse konnten innerhalb des Untersuchungsmaterials 11 Artengruppen ausgeschieden werden. Diskriminanz-Analysen unter Verwendung von allen 19 Variablen ergaben eine Fehlklassifikation beim Versuch der Zuordnung einzelner Samen in 25 % der Fälle. Ein Schlüssel zum Bestimmen der Arten vorzugsweise an Hand von quantitativen Samenmerkmalen wurde vorgeschlagen. Dendrogramme illustrieren die Ergebnisse der Cluster-Analysen und schematische Samendiagramme die Ausprägung der relevanten Samenmerkmale.

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Vicia

19 52 Vicia. : , , . , , 11 . , 19 25 % . , . , — .

     

A rapid liquid chromatography electrospray ionization mass spectrometry(n) method for evaluation of synephrine in Citrus aurantium L. samples

Immature bitter orange fruit and its extracts have been introduced into the market as an alternative to Ephedra in weight loss products. However, the safety of the immature bitter orange fruit and its extracts is a debated argument due to the presence of synephrine, a constituent known as a sympathomimetic agent. In this paper, we describe the development of a new, rapid, and simple liquid chromatography-electrospray ionization-tandem mass spectrometry method devoted to the quantitative determination of synephrine in bitter orange samples, containing a high quantity of synephrine, and sweet orange samples, known to contain a low level of synephrine but at the same time being one of the main synephrine sources in a normal human diet. Two bitter orange dry extracts containing 5 and 6% sSynephrine and 10 sweet orange samples have been analyzed. Between the sweet orange samples, six were fresh oranges and four were fresh-squeezed juices; in these samples, the synephrine levels ranged from 0.00128 to 0.00349%.     

New data on the aberrant spermatogenesis of Collembola

Aberrant spermatogenesis in the two species Stenognathellus denisi (Katiannidae) and Sminthurides aquaticus (Sminthurididae) is described. An ultrastructural study of spermatogenesis in these species has allowed us to reveal the presence of two types of secondary spermatocytes; one with a normal appearance, the other one is characterized by a small size, a reduced cytoplasm (showing only two centrioles), and a nucleus with very condensed chromatin. These latter aberrant cells are not able to perform the second meiotic division and will degenerate. This finding allows us to extend the identification of an aberrant spermatogenesis and the consequent post-zygotic sex determination to two more families of Symphypleona.     

Contribution to the taxonomy ofVicia species belonging to the sectionFaba

Summary Vicia faba belongs to the sectionFaba. While the other species of the sectionV. narbonensis, V. johannis andV. bithynica cross enough well each other,V. faba seems to be strongly isolated from them. In addition the chromosome morphology ofV. faba differs from the other species of the section which among themselves are enough homogenous in this respect.To contribute to a wider knowledge on the taxonomical position ofV. faba in its section, electrophoretical and karyological analysis of 500 individuals belonging to 50 populations of different origin were carried out.The analyzed species were:V. faba, V. narbonensis, V. serratifolia, V. bithynica, V. galilaea andV. johannis.

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Beitrag zur Taxonomie derVicia-Arten der SektionFaba

Zusammenfassung Vicia faba gehört zur sect.Faba. Während andere Arten der Sektion (V. narbonensis, V. johannis undV. bithynica) untereinander relativ gut kreuzbar sind, istV. faba von ihnen deutlich isoliert. Darüberhinaus unterscheidet sichV. faba in der Morphologie seiner Chromosomen von den anderen Arten der Sektion, die in dieser Hinsicht eine relativ einheitliche Merkmalsausprägung zeigen. Um zu einer besseren Kenntnis der systematischen Stellung vonV. faba innerhalb der Sektion beizutragen, wurden chromatographische und karyologische Analysen von 500 Individuen aus 50 Populationen verschiedener Herkunft durchgeführt. Analysiert wurdenV. faba, V. narbonensis, V. serratifolia, V. bithynica, V. galilaea undV. johannis.

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Vicia, Faba

Vicia faba Faba. (V. narbonensis, V. johannis, V. bithynica) ,V. faba ë . ,V. faba , . V. faba , 500 50 . :V. faba, V. narbonensis, V. serratifolia, V. bithynica, V. galilaea, V. johannis.

     

Potential of acrylamide formation,sugars, and free asparagine in potatoes: a comparison of cultivars and farming systems

Glucose, fructose, sucrose, free asparagine, and free glutamine were analyzed in 74 potato samples from 17 potato cultivars grown in 2002 at various locations in Switzerland and different farming systems. The potential of these potatoes for acrylamide formation was measured with a standardized heat treatment. These potentials correlated well with the product of the concentrations of reducing sugars and asparagine. Glucose and fructose were found to determine acrylamide formation. The cultivars showed large differences in their potential of acrylamide formation which was primarily related to their sugar contents. Agricultural practice neither influenced sugars and free asparagine nor the potential of acrylamide formation. It is concluded that acrylamide contents in potato products can be substantially reduced primarily by selecting cultivars with low concentrations of reducing sugars.     

Anti-Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers

This study evaluated the association between plasma anti-Mullerian hormone (AMH) concentration and fertility in Nelore (Bos indicus) heifers submitted to timed artificial insemination (TAI). At the onset of the synchronization protocol, heifers (n = 289) received a subcutaneous P4 ear implant (3 mg) and 2 mg of oestradiol benzoate. Eight days later, the P4 implant was removed and 0.5 mg of oestradiol cypionate, prostaglandin (0.265 mg, i.m.) and equine chorionic gonadotropin (300 UI, i.m.) was administered, and TAI was performed 48 hr after ear implant removal. Ovarian ultrasound evaluations were performed to measure number of ovarian follicles, dominant follicle size and ovulation response. Pregnancy diagnosis was performed by ultrasound 30 days after AI. Heifers with greater circulating AMH had more antral follicles, a smaller dominant follicle near timed ovulation and lower ovulation response to the timed AI protocol compared to heifers with lower circulating AMH. Although AMH and pregnancy outcome had a quadratic-shaped pattern, AMH was not significantly associated with fertility. In conclusion, heifers with lower AMH had larger follicles towards the end of the synchronization protocol and greater ovulation responses, whereas greater circulating AMH was unrelated to conception success.     

A check-list of the cultivated plants of the Ghāt oases

Summary The Ght oases are rich in plant genetic resources. During a mission in 1983 cultivated plants of this area have been studied and altogether 57 accessions of land-races collected. Compiling the results from the collections, from observations all over the Fezzan and from literature studies a check list of the cultivated plants of the Ght oases is presented. Close relationships can be demonstrated especially to land-races from Ethiopia but also from other parts of Africa. The plant genetic resources of the Ght oases may be useful in breeding programmes.

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Eine Liste der Kulturpflanzen der Ght-Oasen

Zusammenfassung Die Ght-Oasen sind reich an pflanzlichen genetischen Ressourcen. Während einer Reise im Jahre 1983 wurden die Kulturpflanzen dieses Gebietes studiert und insgesamt 57 Muster gesammelt. Aus den Ergebnissen der Sammlungen, der Beobachtungen aus dem gesamten Fezzan-Gebiet und der Literaturstudien wird eine Liste der Kulturpflanzen der Ght-Oasen zusammengestellt. Enge Beziehungen können besonders zu den Landsorten Äthiopiens aber auch anderer Teile Afrikas postuliert werden. Die pflanzlichen genetischen Ressourcen der Ght-Oasen stellen ein wertvolles Ausgangsmaterial für die Pflanzenzüchtung dar.

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Characterization of Some Italian Common Bean (<Emphasis Type="Italic">Phaseolus Vulgaris</Emphasis> L.) Landraces by RAPD,Semi-random and ISSR Molecular Markers

Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and a semi-random PCR system were used to analyze the genetic diversity of 16 Italian common bean landraces and their relationship to four commercial cultivars. Of the primers tested, 8 ISSR, 6 RAPD and 7 semi-random primers produced polymorphic and reproducible DNA fragments. A higher proportion of polymorphic bands were observed using ISSR (85%) and semi-random (90%) primers than RAPD (69%) method. The combination of any two semi-random markers allowed the identification of all 20 bean genotypes. In contrast ISSR (except for primer (CAC)₃GC) and RAPD markers appeared to be less informative as more than two markers were necessary to achieve the same diagnostic level. Moreover, 7 ISSR, 2 RAPD and 8 semi-random exclusive bands were identified as putative population-specific markers. Semi-random and ISSR derived dendrograms showed similar tendencies in terms of genetic relatedness, whereas clustering of genotypes within groups was not similar when compared with the RAPD technique. Despite the different ability to resolve genetic variation among the investigated landraces, two major clusters with less than 60% (ISSR) and 40% (RAPD and semi-random) genetic similarity were formed with all three marker systems. The two groups were correlated with the phaseolin patterns and seed size of the landraces. The analysis showed that the cultivar ȁ8Lingua di Fuocoȁ9 and most of the landraces (13 out of 16) collected in Italy belong to the Andean gene pool, whereas only the three populations from Pratomagno belong to the Middle American gene pool.