Profiling glucosinolates, flavonoids, alkaloids, and other secondary metabolites in tissues of Azima tetracantha L. (Salvadoraceae)

Azima tetracantha L. (needle bush; bee sting bush; Salvadoraceae) is used as a food and for various herbal medicines in Africa, India, and Madagascar, but there is very little information on the secondary metabolites in this species. High concentrations of N-methoxy-3-indolylmethyl-glucosinolate, a common glucosinolate of Brassica crops such as Brussels sprouts and broccoli, were found in the roots and seeds of A. tetracantha. Lower concentrations were detected in the stems and young leaves. The roots also contained another indole glucosinolate that was provisionally identified, from MS data and comparison with indole glucosinolate standards, as N-hydroxy-3-indolymethyl-glucosinolate. The roots, stems, and leaves contained neoascorbigen (the condensation product of N-methoxy-indole-3-carbinol and ascorbic acid). The seeds of A. tetracantha contained a complex mixture of 26 flavonoids predominantly as glycosides and acyl-glycosides, with traces of aglycones. The core aglycones of these flavonoids were identified as quercetin, isorhamnetin (3'-O-methylquercetin), rhamnetin (7-O-methylquercetin), and rhamnazin (7, 3'-di-O-methylquercetin). No flavonoids or anthocyanins were detected in other tissues, and procyanidins were undetectable. The dimeric piperidine alkaloids azimine, azcarpine, and carpaine were found in all tissues of A. tetracantha.     

Laparoscopic-assisted castration of a monorchid male pseudohermaphrodite pony

A pseudohermaphrodite possesses gonads of one sex, but with external genitalia of the opposite sex. Although cases of male pseudohermaphroditism have been published, there is no report of a monorchid male pseudohermaphrodite. This Case Report documents a 12-year-old monorchid male pseudohermaphrodite displaying stallion-like behaviour, with an elevated serum oestrone sulphate concentration confirming the presence of functional testicular tissue. A complete resolution of all behavioural signs occurred after laparoscopic-assisted castration under general anaesthesia, along with a drop in serum oestrone sulphate concentrations to <0.01 ng/ml and no response in serum testosterone concentration following administration of hCG.     

Techniques for pollution monitoring in remote sites: III. Near real-time monitoring of cloud water conductivity and pH

A semi-portable flow-through cloud water monitoring system was developed for measurements of cloud water conductivity and pH in remote sites lacking AC line power. This system was tested from May to September on Camels Hump mountain, Vermont. High temporal resolution data from seven cloud events were collected during the 1991 growing season. Mean cloud water conductivity and pH for all events was 467 μmhos cm^?1 and 3.2, respectively. The highest conductivity was 997 (μmhos cm^?1 recorded on 19 September 1991 and the lowest pH of 2.9 was recorded during several events over the summer. Data from this system may be used to achieve a better understanding of the chemical environment in areas experiencing forest decline.     

Cosmogenic and primordial radionuclides in lunar samples by nondestructive gamma-ray spectrometry

The (7)Be, (22)Na, (26)Al, (44)Ti, (46)SC, (48)V (51)Cr, (54)Mn, (56)Co, (57)Co, (57)CO, (40)K, (238)U, and (232)Th were measured in lunar fines and portions of three rocks. Major production of cosmogenic radionuclides is due to solar protons, thus their concentrations are far different than those in meteorites. Surface exposures of the rocks and fines are long compared with the 0.74 million year half-life of (26)Al. Lunar fines show substantially higher concentrations of low energy reaction products. The ratios of thorium to uranium are extremely constant at 3.8, which indicates very little geochemical differentiation and are in good agreement with a common nucleosynthesis for lunar and earth materials.     

New ways to study developmental genes in spore-forming bacteria

The regulated activation of numerous sets of genes in multiple chromosomal locations is a hallmark of cellular differentiation in both eukaryotes and prokaryotes. Certain species of bacteria that experience complex developmental cycles are especially attractive as systems in which to study the mechanisms of this kind of gene regulation because they are highly amenable to both biochemical and genetic approaches. Bacillus subtilis, which undergoes extensive cellular differentiation when it sporulates, is one such system. Many new methods are now available in this Gram-positive species for identifying, manipulating, and studying the regulation of genes involved in spore formation, including the use of transposable genetic elements that create gene fusions in vivo as an automatic consequence of insertions into genes.     

The oxygen resources of the hypolimnion of ionically enriched onondaga lake,NY, U.S.A.

The depletion of hypolimnetic DO and the upper depth boundary of anoxia for four different years (1978, 1979, 1980 and 1981), and the accumulation of sulfide for a single year (1981), are documented for ionically enriched hypereutrophic Onondaga Lake, NY, USA. The depletion rate, represented as the areal hypolimnetic oxygen deficit (AHOD, g m^?2 day^?1), was extremely high (1.2–2.7 g m^?2 day^?1), The large differences in the rate within individual years and from year to year were largely a result of differences in attendant vertical mixing (parameterized as the hypolimnetic heating rate). The entire hypolimnion (depth interval from 11 to 20 m) was without O₂ by late June of all 4 yr; anoxia was observed above the hypolimnion on some occasions when secondary stratification occurred. Sulfide accumulated progressively in the hypolimnion in 1981 following the onset of anoxia to a volume weighted concentration of I1 mg L^?1. The ionic discharge from an adjoining alkali manufacturer exacerbated the problem of limited O₂ resources of the hypolimnion by: (1) decreasing vertical mixing, (2) prolonging the duration of stratification, (3) causing abbreviated turnovers, and (4) encouraging increased rates of phytoplankton settling.     

女人三国

4.0、3.0和2.0,她们之间本不应该有交点,但因为对网球的痴迷程度都是7.0,于是这三个在各自圈子里红得发烫的人,走到了一起。