A survey on Tetracapsuloides bryosalmonae infections in Slovene fresh waters

Slovenia has no history of health problems related to proliferative kidney disease (PKD) either in farmed or in wild fish. However, due to the past molecular evidence for the presence of Tetracapsuloides bryosalmonae DNA in tissues of some fish from open waters, a survey was conducted on wild salmonids that were primarily sampled for other purposes. In winter 2010–2011, specimens from a total of 244 rainbow trout, Oncorhynchus mykiss (Walbaum), and brown trout, Salmo trutta L., from 30 bodies of fresh water were examined for T. bryosalmonae using a PCR method. The sampled fish showed no clinical signs or gross pathological lesions characteristic of PKD. Nineteen (7.8%) fish from seven (23.3%) fresh waters were positive for T. bryosalmonae. The identity of PCR amplicons was confirmed by sequencing. With one exception, all the positive fish were found in waters from the regions where the average yearly temperatures and the environmental pollution are higher. This preliminary countrywide survey provided the first insight into the situation regarding T. bryosalmonae infection of wild salmonids in Slovenia.     

Assessing the performance of woody plants in uptake and utilisation of carbon, water and nutrients: Implications for designing agricultural mimic systems

This article introduces a series of innovative experimental approaches for monitoring current and past performances of woody plant species in utilisation of key resources within ecosystems. We demonstrate how such information might be useful for monitoring the progress of any novel agricultural system which attempts to mimic such natural systems. The experimental data presented principally involve the use of stable isotope techniques coupled to collection and analysis of substances transported in the sap of xylem and phloem. Study species examined are sugar maple (Acer saccharum), tagasaste (Chamaecytisus proliferus), blue gum (Eucalyptus globulus), and acorn banksia (Banksia prionotes). Topics covered include investigations of (a) seasonal changes in water source utilisation using hydrogen stable isotope analyses, (b) water loss from tree roots to the surrounding soil (termed hydraulic lift) using hydrogen stable isotope analyses, (c) relationships between carbon isotope discrimination of plant dry matter and whole-plant water-use efficiency, (d) phloem sap analysis as a means for assessing aspects of a plant's water and nutrient status, (e) carbon isotope ratios of foliage and annual growth rings as they relate to seasonal changes in carbon isotope ratios of phloem translocate, and (f) the composition of simultaneously collected samples of xylem and phloem sap as a means of monitoring current plant performance in uptake and mobilisation of key mineral resources. The chapter concludes with discussion of prospective uses of the above- mentioned techniques in study of managed ecosystems in which woody species play pivotal roles in maintenance of functional stability. This revised version was published online in June 2006 with corrections to the Cover Date.     

Closing of the nucleotide pocket of kinesin-family motors upon binding to microtubules

We have used adenosine diphosphate analogs containing electron paramagnetic resonance (EPR) spin moieties and EPR spectroscopy to show that the nucleotide-binding site of kinesin-family motors closes when the motor.diphosphate complex binds to microtubules. Structural analyses demonstrate that a domain movement in the switch 1 region at the nucleotide site, homologous to domain movements in the switch 1 region in the G proteins [heterotrimeric guanine nucleotide-binding proteins], explains the EPR data. The switch movement primes the motor both for the free energy-yielding nucleotide hydrolysis reaction and for subsequent conformational changes that are crucial for the generation of force and directed motion along the microtubule.     

Relationship of fertility to ovarian follicular waves before breeding in dairy cows

Cows with two waves of follicular growth during the estrous cycle yield follicles that are older and larger at ovulation compared with cows having three waves. The objectives of the current research were 1) to compare fertility in cows with two or three follicular waves and 2) to examine associations between luteal function, follicular development, and fertility after breeding. Follicular waves were monitored by ultrasonography during the estrous cycle before insemination in 106 dairy cows. Fewer cows had three follicular waves before next estrus and ovulation than two waves (P < 0.01; 30% vs 68%, respectively), but pregnancy rate was higher (P = 0.058; 81 vs 63%, respectively). Cows with two waves had shorter estrous cycles (P < 0.01), with the ovulatory follicle being both larger (P < 0.05) and older (P < 0.01). In cows with three waves, luteal function was extended (P < 0.05) and the peak in plasma progesterone occurred later (P < 0.05) in the estrous cycle compared to two wave cows. Considering cows that became pregnant, luteal phase length was shorter (P < 0.05) during the estrous cycle preceding insemination than for nonpregnant cows. In conclusion, fertility was greater in lactating cows inseminated after ovulation of the third-wave follicle that had developed for fewer days of the estrous cycle as compared with two-wave cows.     

Cryptococcosis in seven horses

The clinical, radiographic and post-mortem findings in 6 horses with cryptococcal pneumonia and one horse with an abdominal cryptococcal granuloma are described. In pulmonary cryptococcosis, the lesions were either diffuse and multiple, with bilateral lung involvement, or localised mainly to the dorsocaudal region of one lung. The cases of diffuse multiple cryptococcosis were thought to be associated with haematogenous spread of the fungus after gastrointestinal infection and dissemination from regional lymph nodes. The localised form of the disease was thought to have been associated with inhalation of cryptococci. In all cases of pulmonary cryptococcosis, encapsulated yeast-like organisms were demonstrated in Wright's-stained sediment of tracheal washes. In the horse with the abdominal granuloma, cryptococci were present in a fine needle aspirate sample. Isolates of Cryptococcus neoformans var gattii were recovered from 2 of the 5 horses in which cultures were attempted. In addition to a history of previous illness that may have predisposed to infection, most horses in this report had been in areas in which Eucalyptus camaldulensis, or the closely related E rudis, were growing. In humans, an epidemiological relationship between E camaldulensis and infection with C neoformans var gattii has been suggested. Cases of equine cryptococcosis carry a poor prognosis and treatment was not attempted in any of these cases.     

Effect of calf weaning age and cow supplementation on cow productivity

Five years of data were collected on 124 Brangus-type cows to evaluate weaning calves at 8.5 or 10.5 mo of age and winter supplementation of the brood cow with 2.25 kg of molasses daily. Cows with calves weaned at 10.5 mg of age gained 14 kg less weight (P less than .01) during the late nursing period than did cows from which calves were earlier weaned. Age of calf at weaning had no effect on cow reproduction, but calves weaned at 10.5 mo were 2 to 3 d younger (P less than .06) and 5.3 kg lighter (P less than .05) at 8.5 mo of age. During the last 2 mo of nursing, calves weaned at 10.5 mo of age gained 37.2 kg and had a 31.9-kg heavier weaning weight than calves weaned at 8.5 mo of age. Molasses supplementation resulted in differences (P less than .01) in cow weight changes. Cows fed molasses had calving percentages 5 to 7 units higher (P less than .30) than cows not fed molasses. Feeding cows molasses for 145 d throughout the calving and breeding season increased calf weaning weight 7.7 (P less than .02) and 11.2 (P less than .03) kg, respectively, at 8.5 and 10.5 mo of age over the nonsupplemented controls.